PMID- 31392690
OWN - NLM
STAT- MEDLINE
DCOM- 20200401
LR  - 20200401
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2034
DP  - 2019
TI  - Labeling Microglia with Genetically Encoded Calcium Indicators.
PG  - 243-265
LID - 10.1007/978-1-4939-9658-2_18 [doi]
AB  - Genetically encoded calcium indicators (GECIs) have become widely used for Ca(2+) 
      imaging in cultured cells as well as in living organisms. Transduction of 
      microglia with viral vectors encoding GECIs provides a convenient means to label 
      microglia for in vivo Ca(2+) imaging. We describe a method using 
      microglia-specific microRNA-9-regulated viral vector, to label microglial cells 
      with a ratiometric GECI (Twitch-2B). This method enables longitudinal recording 
      of both transient and sustained elevations of Ca(2+) in microglia in live 
      animals.
FAU - Liang, Yajie
AU  - Liang Y
AD  - Department of Neurophysiology, Institute of Physiology, Eberhard Karls University 
      of Tubingen, Tubingen, Germany.
FAU - Garaschuk, Olga
AU  - Garaschuk O
AD  - Department of Neurophysiology, Institute of Physiology, Eberhard Karls University 
      of Tubingen, Tubingen, Germany. olga.garaschuk@uni-tuebingen.de.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Luminescent Proteins)
RN  - 0 (MIRN9 microRNA, mouse)
RN  - 0 (MicroRNAs)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/*metabolism
MH  - *Calcium Signaling
MH  - *Genetic Vectors
MH  - HEK293 Cells
MH  - Humans
MH  - *Lentivirus
MH  - *Luminescent Proteins/biosynthesis/genetics
MH  - Mice
MH  - MicroRNAs/biosynthesis/genetics
MH  - *Microglia/cytology/metabolism
MH  - *Staining and Labeling
MH  - *Transduction, Genetic
MH  - *Transgenes
OTO - NOTNLM
OT  - Basal Ca2+ level
OT  - Calcium imaging
OT  - Genetically encoded calcium indicator
OT  - MicroRNA
OT  - Microglia
OT  - Transduction
EDAT- 2019/08/09 06:00
MHDA- 2020/04/02 06:00
CRDT- 2019/08/09 06:00
PHST- 2019/08/09 06:00 [entrez]
PHST- 2019/08/09 06:00 [pubmed]
PHST- 2020/04/02 06:00 [medline]
AID - 10.1007/978-1-4939-9658-2_18 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2019;2034:243-265. doi: 10.1007/978-1-4939-9658-2_18.