PMID- 31394648
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20190809
IS  - 1790-6245 (Electronic)
IS  - 1109-6535 (Linking)
VI  - 2
IP  - 6
DP  - 2005 Nov-Dec
TI  - Genomic Instability, DNA Alterations and Tumor Eosinophilic Expression in Head 
      and Neck Squamous Cell Carcinoma.
PG  - 307-316
AB  - The progression of normal cells to invasive tumor cells has been attributed to 
      the acquisition of numerous mutations in the genome; genomic instability (GI) 
      facilitates the accumulation of these mutations. To study the GI in head and neck 
      squamous cell carcinoma (HNSCC), the genomic instability index (GII) was 
      examined; chromosomal gains and losses were evaluated by array comparative 
      genomic hybridization (aCGH), which were confirmed by fluorescence in situ 
      hybridization (FISH). Histopathological eosinophilic infiltrate (Eos Infil), as 
      an adjunct measure of tumor invasiveness, was also considered and compared to GI. 
      MATERIALS AND METHODS: Inter-simple sequence repeat PCR (ISSR-PCR) was utilized 
      to determine the GII (a quantitative estimate of the relative overall genomic 
      damage). GII was measured in 26 pairs of tumor and normal HNSCC samples. Array 
      CGH was conducted using bacterial artificial chromosome (BAC) clones to evaluate 
      amplifications and deletions (n=20 tumor), and confirmation of the specific 
      changes was made by FISH analysis. Histopathological evaluation of Eos Infil for 
      all samples was performed to calculate the eosinophilic index (EI). This was 
      accomplished by observing Eos Infil in neoplastic tissue and at the tumor/normal 
      tissue interface. RESULTS: GI was evident in 25 of the 26 tumors. The GIIs ranged 
      from 0 to 5.3% with a mean of 2.8% (similar to the results reported for 
      colorectal and thyroid carcinomas). GIIs were higher in tumors of the oral cavity 
      (OC) than in tumors from other subsites of HNSCC (p=0.05). Chromosomal 
      alterations were identified by array CGH on chromosomes 8, 11 and 17, but 
      consistent amplifications were observed on (8q21.3-23.5) in 80% of the tumors. 
      These changes were confirmed using FISH analysis. There was an association 
      between increased GI and rising EI, but this did not achieve statistical 
      significance (p>0.05). CONCLUSION: HNSCC exhibits a similar degree of GI to that 
      previously reported in colorectal and thyroid malignancies. The higher GI 
      identified in OC tumors could be explained by a greater degree of the damage from 
      environmental carcinogens (smoke, diet and alcohol) to the first station of the 
      areodigestive tract. Consistent amplification at the specific loci of 8q might be 
      attributed to mutations in various genes, such as SHAX3 (Snf7 homolog, associated 
      with Alix 3, involved in apoptosis and endocytosis) and E2F5 (transcription 
      factor 5 that interacts with tumor suppressor proteins). EI was not associated 
      with age, sex, site or stage of tumor in established cases. Further work up will 
      be necessary to explain these results.
CI  - Copyright(c) 2005 International Institute of Anticaner Research (Dr. John G. 
      Delinassios), All rights reserved.
FAU - Alrawi, Sadir J
AU  - Alrawi SJ
AD  - Department of Head and Neck/Surgical Oncology, Roswell Park Cancer Institute, 
      Buffalo, New York Sadir.Alrawi@RoswellPark.org.
FAU - Stoler, Daniel
AU  - Stoler D
AD  - Department of Cancer Genetics, Roswell Park Cancer Institute, Buffalo, New York.
FAU - Tan, Dongfeng
AU  - Tan D
AD  - Department of Pathology, Roswell Park Cancer Institute, Buffalo, New York.
FAU - Dayton, Merril
AU  - Dayton M
AD  - Department of Surgery, University of Buffalo, SUNY, New York, U.S.A.
FAU - Loree, Thom
AU  - Loree T
AD  - Department of Head and Neck/Surgical Oncology, Roswell Park Cancer Institute, 
      Buffalo, New York.
FAU - Gibbs, John F
AU  - Gibbs JF
AD  - Department of Head and Neck/Surgical Oncology, Roswell Park Cancer Institute, 
      Buffalo, New York.
FAU - Rigual, Nestor
AU  - Rigual N
AD  - Department of Head and Neck/Surgical Oncology, Roswell Park Cancer Institute, 
      Buffalo, New York.
FAU - Sait, Sheila
AU  - Sait S
AD  - Department of Pathology, Roswell Park Cancer Institute, Buffalo, New York.
FAU - Khoury, Thaer
AU  - Khoury T
AD  - Department of Pathology, Roswell Park Cancer Institute, Buffalo, New York.
FAU - Hicks, Wesley Jr
AU  - Hicks W Jr
AD  - Department of Head and Neck/Surgical Oncology, Roswell Park Cancer Institute, 
      Buffalo, New York.
FAU - Anderson, Garth
AU  - Anderson G
AD  - Department of Cancer Genetics, Roswell Park Cancer Institute, Buffalo, New York.
LA  - eng
PT  - Journal Article
DEP - 20051101
PL  - Greece
TA  - Cancer Genomics Proteomics
JT  - Cancer genomics & proteomics
JID - 101188791
SB  - IM
OTO - NOTNLM
OT  - Head and neck squamous cell carcinoma
OT  - comparative genomic hybridization
OT  - eosinophilic index
OT  - eosinophilic infiltrate
OT  - fluorescence in situ hybridization
OT  - genomic instability
OT  - genomic instability index
OT  - oral cavity
EDAT- 2005/11/01 00:00
MHDA- 2005/11/01 00:01
CRDT- 2019/08/10 06:00
PHST- 2005/07/08 00:00 [received]
PHST- 2005/10/03 00:00 [revised]
PHST- 2005/10/10 00:00 [accepted]
PHST- 2019/08/10 06:00 [entrez]
PHST- 2005/11/01 00:00 [pubmed]
PHST- 2005/11/01 00:01 [medline]
AID - 2/6/307 [pii]
PST - ppublish
SO  - Cancer Genomics Proteomics. 2005 Nov-Dec;2(6):307-316. Epub 2005 Nov 1.