PMID- 31395377 OWN - NLM STAT- MEDLINE DCOM- 20200504 LR - 20200505 IS - 0091-679X (Print) IS - 0091-679X (Linking) VI - 153 DP - 2019 TI - Studying Na(+) and K(+) channels in aldosterone-sensitive distal nephrons. PG - 151-168 LID - S0091-679X(19)30060-3 [pii] LID - 10.1016/bs.mcb.2019.04.009 [doi] AB - Aldosterone-sensitive distal nephron (ASDN) including the distal convoluted tubule (DCT), connecting tubule (CNT) and collecting duct (CD) plays an important role in the regulation of hormone-dependent Na(+) reabsorption and dietary K(+)-intake dependent K(+) excretion. The major Na(+) transporters in the ASDN are thiazide-sensitive Na-Cl cotransporter (NCC), epithelial Na(+) channel (ENaC), pendrin/Na(+)-dependent Cl(-)-bicarbonate exchanger (NDCBE). Whereas major K(+) channels in the ASDN are Kir4.1 and Kir5.1 in the basolateral membrane; and Kir1.1 (ROMK) and Ca(2+) activated big conductance K(+) channel (BK) in the apical membrane. Although a variety of in vitro cell lines of the ASDN is available and these cell models have been employed for studying Na(+) and K(+) channels, the biophysical properties and the regulation of Na(+) and K(+) channels in vitro cell models may not be able to recapitulate those in vivo conditions. Thus, the studies performed in the native ASDN are essential for providing highly physiological relevant information and for understanding the Na(+) and K(+) transport in the ASDN. Here we provide a detailed methodology describing how to perform the electrophysiological measurement in the native DCT, CNT and cortical collecting duct (CCD). CI - (c) 2019 Elsevier Inc. All rights reserved. FAU - Teulon, Jacques AU - Teulon J AD - Sorbnne Universite, Centre de recherches des Cordeliers UMR_S 1138, equipe 3, Paris, France. Electronic address: jacques.teulon@crc.jussieu.fr. FAU - Wang, Wen-Hui AU - Wang WH AD - Department of Pharmacology, New York Medical College, Valhalla, NY, United States. Electronic address: wenhui_wang@nymc.edu. LA - eng PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't DEP - 20190522 PL - United States TA - Methods Cell Biol JT - Methods in cell biology JID - 0373334 RN - 0 (Cations, Monovalent) RN - 0 (Ion Channels) RN - 4964P6T9RB (Aldosterone) RN - 9NEZ333N27 (Sodium) RN - RWP5GA015D (Potassium) SB - IM MH - Aldosterone/metabolism MH - Animals MH - Cations, Monovalent/metabolism MH - Ion Channels/*metabolism MH - Kidney Tubules, Distal/*metabolism MH - Mice MH - Microdissection/instrumentation/methods MH - Patch-Clamp Techniques/instrumentation/*methods MH - Potassium/*metabolism MH - Renal Elimination/physiology MH - Renal Reabsorption/physiology MH - Sodium/*metabolism OTO - NOTNLM OT - CCD OT - CNT OT - ClC-kb OT - DCT OT - ENaC OT - Kir4.1 OT - Kir5.1 OT - ROMK EDAT- 2019/08/10 06:00 MHDA- 2020/05/06 06:00 CRDT- 2019/08/10 06:00 PHST- 2019/08/10 06:00 [entrez] PHST- 2019/08/10 06:00 [pubmed] PHST- 2020/05/06 06:00 [medline] AID - S0091-679X(19)30060-3 [pii] AID - 10.1016/bs.mcb.2019.04.009 [doi] PST - ppublish SO - Methods Cell Biol. 2019;153:151-168. doi: 10.1016/bs.mcb.2019.04.009. Epub 2019 May 22.