PMID- 31395919
OWN - NLM
STAT- MEDLINE
DCOM- 20201113
LR  - 20210110
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 9
IP  - 1
DP  - 2019 Aug 8
TI  - Biogeochemical Changes During Bio-cementation Mediated by Stimulated and 
      Augmented Ureolytic Microorganisms.
PG  - 11517
LID - 10.1038/s41598-019-47973-0 [doi]
LID - 11517
AB  - Microbially Induced Calcite Precipitation (MICP) is a bio-mediated cementation 
      process that can improve the engineering properties of granular soils through the 
      precipitation of calcite. The process is made possible by soil microorganisms 
      containing urease enzymes, which hydrolyze urea and enable carbonate ions to 
      become available for precipitation. While most researchers have injected 
      non-native ureolytic bacteria to complete bio-cementation, enrichment of native 
      ureolytic microorganisms may enable reductions in process treatment costs and 
      environmental impacts. In this study, a large-scale bio-cementation experiment 
      involving two 1.7-meter diameter tanks and a complementary soil column experiment 
      were performed to investigate biogeochemical differences between bio-cementation 
      mediated by either native or augmented (Sporosarcina pasteurii) ureolytic 
      microorganisms. Although post-treatment distributions of calcite and engineering 
      properties were similar between approaches, the results of this study suggest 
      that significant differences in ureolysis rates and related precipitation rates 
      between native and augmented microbial communities may influence the temporal 
      progression and spatial distribution of bio-cementation, solution biogeochemical 
      changes, and precipitate microstructure. The role of urea hydrolysis in enabling 
      calcite precipitation through sustained super-saturation following treatment 
      injections is explored.
FAU - Gomez, Michael G
AU  - Gomez MG
AUID- ORCID: 0000-0002-4464-5447
AD  - Department of Civil and Environmental Engineering, University of Washington, 
      Seattle, WA, 98195, USA. mggomez@uw.edu.
FAU - Graddy, Charles M R
AU  - Graddy CMR
AUID- ORCID: 0000-0002-4432-9823
AD  - Department of Microbiology and Molecular Genetics, University of California, 
      Davis, CA, 95616, USA.
FAU - DeJong, Jason T
AU  - DeJong JT
AUID- ORCID: 0000-0002-9809-955X
AD  - Department of Civil and Environmental Engineering, University of California, 
      Davis, CA, 95616, USA.
FAU - Nelson, Douglas C
AU  - Nelson DC
AUID- ORCID: 0000-0001-5878-6868
AD  - Department of Microbiology and Molecular Genetics, University of California, 
      Davis, CA, 95616, USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20190808
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - EC 3.5.1.5 (Urease)
RN  - H0G9379FGK (Calcium Carbonate)
SB  - IM
MH  - Bacteria/enzymology/growth & development/*metabolism
MH  - Calcium Carbonate/*metabolism
MH  - Chemical Precipitation
MH  - Colony Count, Microbial
MH  - Hydrolysis
MH  - Microscopy, Electron, Scanning
MH  - Urease/*metabolism
PMC - PMC6687734
COIS- The authors declare no competing interests.
EDAT- 2019/08/10 06:00
MHDA- 2020/11/18 06:00
PMCR- 2019/08/08
CRDT- 2019/08/10 06:00
PHST- 2019/04/05 00:00 [received]
PHST- 2019/07/26 00:00 [accepted]
PHST- 2019/08/10 06:00 [entrez]
PHST- 2019/08/10 06:00 [pubmed]
PHST- 2020/11/18 06:00 [medline]
PHST- 2019/08/08 00:00 [pmc-release]
AID - 10.1038/s41598-019-47973-0 [pii]
AID - 47973 [pii]
AID - 10.1038/s41598-019-47973-0 [doi]
PST - epublish
SO  - Sci Rep. 2019 Aug 8;9(1):11517. doi: 10.1038/s41598-019-47973-0.