PMID- 31396849
OWN - NLM
STAT- MEDLINE
DCOM- 20200320
LR  - 20210110
IS  - 1573-7330 (Electronic)
IS  - 1058-0468 (Print)
IS  - 1058-0468 (Linking)
VI  - 36
IP  - 10
DP  - 2019 Oct
TI  - The use of fluorescence in situ hybridization analysis on sperm: indications to 
      perform and assisted reproduction technology outcomes.
PG  - 1975-1987
LID - 10.1007/s10815-019-01554-2 [doi]
AB  - PURPOSE: To determine the consequences of an altered sperm fluorescence in situ 
      hybridization (FISH) result for ART outcomes and the indications for a sperm FISH 
      analysis. METHODS: Data from 439 infertile men were collected. Bivariate analyses 
      were performed to determine the association of men's age, seminal alterations, 
      and sperm FISH indication, with the incidence of X, Y, 13, 18, and 21 sperm 
      chromosomal abnormalities. A multivariate logistic regression analysis was 
      performed to establish the most predictive variables for altered sperm FISH. 
      Results from the IVF/ICSI cycles were collected for 248 out of 439 patients. Two 
      distinct groups were established: 151 couples that used their own oocytes and 97 
      couples involved in egg donation programs. In both groups, ART outcomes were 
      compared between normal and altered sperm FISH. RESULTS: Teratozoospermia and 
      oligozoospermia were associated with sperm chromosome anomalies (p < 0.05). 
      Indications for sperm FISH analysis with the highest predictability were 
      teratozoospermia, male age, oligozoospermia, and implantation failure (AUC = 
      0.702). Embryo quality (p = 0.096), pregnancy rate (p = 0.054), and implantation 
      rate (p = 0.089) were higher in own-oocytes couples with normal sperm FISH than 
      in altered sperm FISH couples, although differences were not statistically 
      significant. In donor-oocytes couples, in which high-quality embryos were 
      transferred later than in own-oocytes couples (3.8 vs. 3.0 days), we did not 
      identify differences in the ART outcome between normal and altered sperm FISH 
      couples. In both groups, the possible interference of woman age was negligible. 
      CONCLUSIONS: Sperm FISH is indicated in middle-aged oligoteratozoospermic 
      patients with implantation failures in previous IVF/ICSI cycles. Sperm chromosome 
      anomalies have a moderate detrimental impact on embryo quality, implantation, and 
      pregnancy rates.
FAU - Sarrate, Zaida
AU  - Sarrate Z
AD  - Genetics of Male Fertility Group, Unitat de Biologia Cel.lular, Facultat de 
      Biociencies, Universitat Autonoma de Barcelona, 08193, Bellaterra (Cerdanyola del 
      Valles), Spain.
FAU - Blanco, Joan
AU  - Blanco J
AD  - Genetics of Male Fertility Group, Unitat de Biologia Cel.lular, Facultat de 
      Biociencies, Universitat Autonoma de Barcelona, 08193, Bellaterra (Cerdanyola del 
      Valles), Spain. joan.blanco@uab.cat.
FAU - Marina-Rugero, Fernando
AU  - Marina-Rugero F
AD  - Instituto CEFER, c/ Marquesa de Vilallonga 12, 08017, Barcelona, Spain.
FAU - Moreno-Garcia, Juan Manuel
AU  - Moreno-Garcia JM
AD  - UR Clinica Vistahermosa, Avda de Denia 103, 03015, Alicante, Spain.
FAU - Ruiz-Jorro, Miguel
AU  - Ruiz-Jorro M
AD  - Crea Medicina de la Reproduccion, c/ San Martin 4, 46003, Valencia, Spain.
FAU - Lafuente-Varea, Rafael
AU  - Lafuente-Varea R
AD  - CIRH, Plza Eguilaz 14, 08017, Barcelona, Spain.
FAU - Grana-Zanon, Fernando
AU  - Grana-Zanon F
AD  - CEFIVA, Plaza Ferrocarriles Economicos de Asturias 6-8, 33011, Oviedo, Spain.
FAU - Nunez-Calonge, Rocio
AU  - Nunez-Calonge R
AD  - Clinica Tambre, c/ Tambre 8, 28002, Madrid, Spain.
FAU - Ten, Jorge
AU  - Ten J
AD  - Embryology Unit, Instituto Bernabeu, Av. Albufereta 31, 03016, Alicante, Spain.
FAU - Rueda, Joaquin
AU  - Rueda J
AD  - Departamento de Histologia y Anatomia, Unidad de Genetica, Catedra de Biomedicina 
      Reproductiva Clinica Vistahermosa, Universidad Miguel Hernandez, 03110, Sant Joan 
      d'Alacant, Spain.
LA  - eng
PT  - Journal Article
DEP - 20190808
PL  - Netherlands
TA  - J Assist Reprod Genet
JT  - Journal of assisted reproduction and genetics
JID - 9206495
SB  - IM
MH  - Adult
MH  - Chromosome Aberrations
MH  - Embryo Implantation/genetics/physiology
MH  - Female
MH  - Fertilization in Vitro/methods
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Oligospermia/*diagnosis/genetics/pathology
MH  - Pregnancy
MH  - Pregnancy Rate
MH  - Reproductive Techniques, Assisted
MH  - Sperm Injections, Intracytoplasmic/methods
MH  - Spermatozoa/pathology/*ultrastructure
MH  - Teratozoospermia/*diagnosis/genetics/pathology
MH  - Tissue Donors
PMC - PMC6823339
OTO - NOTNLM
OT  - ART outcome
OT  - Blastocyst transfer
OT  - Male age
OT  - Seminal parameters
OT  - Sperm FISH indications
EDAT- 2019/08/10 06:00
MHDA- 2020/03/21 06:00
PMCR- 2020/10/01
CRDT- 2019/08/10 06:00
PHST- 2018/12/02 00:00 [received]
PHST- 2019/07/30 00:00 [accepted]
PHST- 2019/08/10 06:00 [pubmed]
PHST- 2020/03/21 06:00 [medline]
PHST- 2019/08/10 06:00 [entrez]
PHST- 2020/10/01 00:00 [pmc-release]
AID - 10.1007/s10815-019-01554-2 [pii]
AID - 1554 [pii]
AID - 10.1007/s10815-019-01554-2 [doi]
PST - ppublish
SO  - J Assist Reprod Genet. 2019 Oct;36(10):1975-1987. doi: 
      10.1007/s10815-019-01554-2. Epub 2019 Aug 8.