PMID- 31397222
OWN - NLM
STAT- MEDLINE
DCOM- 20200220
LR  - 20200220
IS  - 1743-1328 (Electronic)
IS  - 0161-6412 (Linking)
VI  - 41
IP  - 11
DP  - 2019 Nov
TI  - Relationships between microRNA-20a and microRNA-125b expression and apoptosis and 
      inflammation in experimental spinal cord injury.
PG  - 991-1000
LID - 10.1080/01616412.2019.1652014 [doi]
AB  - Objectives: The aim of the study was to determine the relationships between 
      microRNA-20a and microRNA-125b expression and apoptosis and inflammation in a rat 
      model of spinal cord injury (SCI) using microscopy, immunohistochemistry, and 
      molecular biology. Methods: Sixty-one rats were divided into three groups: a 
      control group that was not subjected to any operation; a sham-operated group; and 
      an experimental group that was subjected to spinal cord compression. The 
      experimental group was further subdivided into two subgroups: the experimental 
      control group, which did not receive any drug treatment; and the 
      methylprednisolone treatment group, which received 30 mg/kg methylprednisolone on 
      day 0 followed by 10 mg/kg/day methylprednisolone from days 1-14. Results: Tumor 
      necrosis factor-alpha (TNF-alpha) and interleukin (IL)-6 levels increased in the 
      experimental control group on days 1 and 3, and decreased in the experimental 
      control group and methylprednisolone treatment group on days 7 and 14. Caspase-3 
      levels increased in the experimental control group on day 1, and decreased in the 
      experimental control group and methylprednisolone treatment group on days 3, 7, 
      and 14. MicroRNA-20a expression was upregulated in the experimental control group 
      on days 1 and 3, and microRNA-125b expression was downregulated on days 3 and 7. 
      Conclusions: After SCI, upregulated microRNA-20a expression and increased 
      proinflammatory cytokines may lead to an increase in inflammation. MicroRNA-125b 
      may be associated with caspase-3, and microRNA-125b downregulation may inhibit 
      apoptosis. Although the results of this study suggest potential relationships 
      between microRNA-20a and microRNA-125b expression and apoptosis and inflammation 
      in SCI, further studies are needed to confirm microRNA-20a and microRNA-125b as 
      biomarkers in SCI and to develop new strategies for the treatment of SCI.
FAU - Saker, Dilek
AU  - Saker D
AD  - Department of Histology and Embryology, Faculty of Medicine, Cukurova University 
      , Adana , Turkey.
FAU - Sencar, Leman
AU  - Sencar L
AD  - Department of Histology and Embryology, Faculty of Medicine, Cukurova University 
      , Adana , Turkey.
FAU - Yilmaz, Dervis Mansuri
AU  - Yilmaz DM
AD  - Department of Neurosurgery, Faculty of Medicine, Cukurova University , Adana , 
      Turkey.
FAU - Polat, Sait
AU  - Polat S
AD  - Department of Histology and Embryology, Faculty of Medicine, Cukurova University 
      , Adana , Turkey.
LA  - eng
PT  - Journal Article
DEP - 20190809
PL  - England
TA  - Neurol Res
JT  - Neurological research
JID - 7905298
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-6)
RN  - 0 (MIRN125 microRNA, rat)
RN  - 0 (MIRN20 microRNA, rat)
RN  - 0 (MicroRNAs)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - X4W7ZR7023 (Methylprednisolone)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects/*genetics
MH  - Cytokines/metabolism
MH  - Down-Regulation/drug effects
MH  - Inflammation/metabolism
MH  - Interleukin-6/metabolism
MH  - Male
MH  - Methylprednisolone/therapeutic use
MH  - MicroRNAs/*genetics
MH  - Rats, Wistar
MH  - Signal Transduction/drug effects
MH  - Spinal Cord/metabolism
MH  - Spinal Cord Injuries/*drug therapy/genetics
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Up-Regulation/drug effects
OTO - NOTNLM
OT  - MicroRNA-20a
OT  - apoptosis
OT  - inflammation
OT  - microRNA-125b
OT  - spinal cord injury
EDAT- 2019/08/10 06:00
MHDA- 2020/02/23 06:00
CRDT- 2019/08/10 06:00
PHST- 2019/08/10 06:00 [pubmed]
PHST- 2020/02/23 06:00 [medline]
PHST- 2019/08/10 06:00 [entrez]
AID - 10.1080/01616412.2019.1652014 [doi]
PST - ppublish
SO  - Neurol Res. 2019 Nov;41(11):991-1000. doi: 10.1080/01616412.2019.1652014. Epub 
      2019 Aug 9.