PMID- 31397323
OWN - NLM
STAT- MEDLINE
DCOM- 20200103
LR  - 20231013
IS  - 2053-230X (Electronic)
IS  - 2053-230X (Linking)
VI  - 75
IP  - Pt 8
DP  - 2019 Aug 1
TI  - AMi: a GUI-based, open-source system for imaging samples in multi-well plates.
PG  - 531-536
LID - 10.1107/S2053230X19009853 [doi]
AB  - Described here are instructions for building and using an inexpensive automated 
      microscope (AMi) that has been specifically designed for viewing and imaging the 
      contents of multi-well plates. The X, Y, Z translation stage is controlled 
      through dedicated software (AMiGUI) that is being made freely available. 
      Movements are controlled by an Arduino-based board running grbl, and the 
      graphical user interface and image acquisition are controlled via a Raspberry Pi 
      microcomputer running Python. Images can be written to the Raspberry Pi or to a 
      remote disk. Plates with multiple sample wells at each row/column position are 
      supported, and a script file for automated z-stack depth-of-field enhancement is 
      written along with the images. The graphical user interface and real-time imaging 
      also make it easy to manually inspect and capture images of individual samples.
CI  - open access.
FAU - Bohm, Andrew
AU  - Bohm A
AD  - Department of Developmental, Molecular, and Chemical Biology, Tufts University, 
      136 Harrison Avenue, Boston, MA 02111, USA.
LA  - eng
GR  - R01 AI150499/AI/NIAID NIH HHS/United States
GR  - R01 GM126149/GM/NIGMS NIH HHS/United States
PT  - Journal Article
DEP - 20190806
PL  - United States
TA  - Acta Crystallogr F Struct Biol Commun
JT  - Acta crystallographica. Section F, Structural biology communications
JID - 101620319
SB  - IM
MH  - *Computer Graphics
MH  - Humans
MH  - Image Processing, Computer-Assisted/*methods
MH  - Microscopy/*instrumentation
MH  - Pattern Recognition, Automated/*methods
MH  - *Software
MH  - *User-Computer Interface
PMC - PMC6688666
OTO - NOTNLM
OT  - AMi
OT  - AMiGUI
OT  - automated microscopy
OT  - crystallography
OT  - multi-well plates
EDAT- 2019/08/10 06:00
MHDA- 2020/01/04 06:00
PMCR- 2019/08/06
CRDT- 2019/08/10 06:00
PHST- 2019/05/24 00:00 [received]
PHST- 2019/07/09 00:00 [accepted]
PHST- 2019/08/10 06:00 [entrez]
PHST- 2019/08/10 06:00 [pubmed]
PHST- 2020/01/04 06:00 [medline]
PHST- 2019/08/06 00:00 [pmc-release]
AID - S2053230X19009853 [pii]
AID - ft5102 [pii]
AID - 10.1107/S2053230X19009853 [doi]
PST - ppublish
SO  - Acta Crystallogr F Struct Biol Commun. 2019 Aug 1;75(Pt 8):531-536. doi: 
      10.1107/S2053230X19009853. Epub 2019 Aug 6.