PMID- 31398419
OWN - NLM
STAT- MEDLINE
DCOM- 20191028
LR  - 20191028
IS  - 1879-0631 (Electronic)
IS  - 0024-3205 (Linking)
VI  - 233
DP  - 2019 Sep 15
TI  - Lysosomal dysfunction is associated with persistent lung injury in dams caused by 
      pregnancy exposure to carbon black nanoparticles.
PG  - 116741
LID - S0024-3205(19)30668-X [pii]
LID - 10.1016/j.lfs.2019.116741 [doi]
AB  - AIMS: Carbon black nanoparticles (CBNPs) are widely used in industrial field. 
      Sensitive stages such as pregnancy are assumed to be more susceptible to 
      stimulus, however whether pregnancy exposure to CBNPs (PrE-to-CBNPs) would cause 
      long-term toxic effects in dams and the underlying mechanisms remain poorly 
      addressed. The present study is aimed to determine the long-term toxic effects of 
      PrE-to-CBNPs in dams. MATERIALS AND METHODS: The pregnant mice were randomly 
      divided into control group, low (21 mug/animal), medium (103 mug/animal) and high 
      (515 mug/animal) CBNPs-treated groups. From gestational day (GD) 9 to GD18, the 
      pregnant mice were intranasal exposed. At 49 days after parturition, lung tissues 
      and bronchoalveolar lavage fluid (BALF) were obtained. Weight change, lung 
      histopathology, lung ultrastructural pathology, cell count in BALF, oxidative 
      stress/inflammatory maker and autophagy/lysosome-related protein expression were 
      determined. KEY FINDINGS: PrE-to-CBNPs caused a dose-dependent persistent lung 
      injury in mice even 49 days after parturition, including the deteriorative lung 
      histopathological changes, elevation of oxidative stress marker Nrf-2, HO-1 and 
      CHOP, infiltration of macrophage and increased mRNA expression of inflammatory 
      cytokines in the lung tissues and elevation of cells in BALF. However, 
      PrE-to-CBNPs did not induce significant neutrophil infiltration and fibrosis. 
      Moreover, we found that CBNPs could deposit in the lysosomes and decrease 
      cathepsin D (an important hydrolase in lysosome), which might be associated with 
      the dysfunction of lysosome and autophagy. SIGNIFICANCE: Our study demonstrated 
      that PrE-to-CBNPs could result in long-term lung injury in dams, and lysosomal 
      dysfunction was probably linked to this process.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Liu, Xuemei
AU  - Liu X
AD  - Department of Occupational and Environmental Health, School of Public Health and 
      Management, Chongqing Medical University, Chongqing 400016, PR China.
FAU - Tu, Baijie
AU  - Tu B
AD  - Department of Occupational and Environmental Health, School of Public Health and 
      Management, Chongqing Medical University, Chongqing 400016, PR China.
FAU - Jiang, Xuejun
AU  - Jiang X
AD  - Center of Experimental Teaching for Public Health, Experimental Teaching and 
      Management Center, Chongqing Medical University, Chongqing 400016, PR China.
FAU - Xu, Ge
AU  - Xu G
AD  - Institute of Life Sciences, Chongqing Medical University, Chongqing 400016, PR 
      China.
FAU - Bai, Lulu
AU  - Bai L
AD  - Department of Occupational and Environmental Health, School of Public Health and 
      Management, Chongqing Medical University, Chongqing 400016, PR China.
FAU - Zhang, Longbin
AU  - Zhang L
AD  - Department of Occupational and Environmental Health, School of Public Health and 
      Management, Chongqing Medical University, Chongqing 400016, PR China.
FAU - Meng, Pan
AU  - Meng P
AD  - Department of Occupational and Environmental Health, School of Public Health and 
      Management, Chongqing Medical University, Chongqing 400016, PR China.
FAU - Qin, Xia
AU  - Qin X
AD  - Department of Pharmacy, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing 400016, PR China.
FAU - Chen, Chengzhi
AU  - Chen C
AD  - Department of Occupational and Environmental Health, School of Public Health and 
      Management, Chongqing Medical University, Chongqing 400016, PR China; Dongsheng 
      Lung-Brain Disease Joint Lab, Chongqing Medical University, Chongqing 400016, PR 
      China. Electronic address: chengzhichen@cqmu.edu.cn.
FAU - Zou, Zhen
AU  - Zou Z
AD  - Institute of Life Sciences, Chongqing Medical University, Chongqing 400016, PR 
      China; Dongsheng Lung-Brain Disease Joint Lab, Chongqing Medical University, 
      Chongqing 400016, PR China. Electronic address: zouzhen@cqmu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20190806
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Cytokines)
RN  - 0 (Soot)
SB  - IM
MH  - Animals
MH  - Autophagy
MH  - Cytokines/metabolism
MH  - Female
MH  - Inflammation/*complications
MH  - Lung Injury/*etiology/pathology
MH  - Lysosomes/*pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Nanoparticles/*adverse effects
MH  - *Oxidative Stress
MH  - Pregnancy
MH  - Prenatal Exposure Delayed Effects/*etiology/pathology
MH  - Soot/*adverse effects
OTO - NOTNLM
OT  - Carbon black nanoparticles
OT  - Lysosomal dysfunction
OT  - Persistent lung injury
OT  - Pregnancy exposure
EDAT- 2019/08/10 06:00
MHDA- 2019/10/29 06:00
CRDT- 2019/08/10 06:00
PHST- 2019/06/17 00:00 [received]
PHST- 2019/07/31 00:00 [revised]
PHST- 2019/08/06 00:00 [accepted]
PHST- 2019/08/10 06:00 [pubmed]
PHST- 2019/10/29 06:00 [medline]
PHST- 2019/08/10 06:00 [entrez]
AID - S0024-3205(19)30668-X [pii]
AID - 10.1016/j.lfs.2019.116741 [doi]
PST - ppublish
SO  - Life Sci. 2019 Sep 15;233:116741. doi: 10.1016/j.lfs.2019.116741. Epub 2019 Aug 
      6.