PMID- 31398529
OWN - NLM
STAT- MEDLINE
DCOM- 20200113
LR  - 20200113
IS  - 1096-1208 (Electronic)
IS  - 0882-4010 (Linking)
VI  - 135
DP  - 2019 Oct
TI  - Prevalence and genetic characteristics of porcine reproductive and respiratory 
      syndrome virus in central China during 2016-2017: NADC30-like PRRSVs are 
      predominant.
PG  - 103657
LID - S0882-4010(18)31198-7 [pii]
LID - 10.1016/j.micpath.2019.103657 [doi]
AB  - NADC30-like strains of porcine reproductive and respiratory syndrome virus 
      (PRRSV) were firstly reported in China in 2013. Since then, these strains have 
      been epidemic in more than 13 provinces/regions. During 2016-2017, a total of 18 
      PRRSV isolates were obtained from 52 clinical samples in Henan province. Based on 
      comparative and phylogenetic analyses of ORF5 and partial Nsp2 genes, 83.3% 
      (15/18) isolates belonged to NADC30-like strains, and the ORF5 shared 87.4%-95.5% 
      nucleotide identity with NADC30/JL580 and 84.2%-89.9% with JXA1/CH-1a, 
      respectively. The genetic variation analysis showed that extensive amino acid 
      substitutions happened in the significant regions of ORF5 including major linear 
      antigenic epitopes (27-30aa, 37-45aa, 52-61aa) and the potential N-glycosylation 
      sites (32-35aa). 16.7% (3/18) isolates were very close to HP-PRRSV derived 
      attenuated strains. Moreover, these three isolates shared common residues at the 
      positions 33D, 59 N, 164R, 196R in ORF5 and 303D, 399T, 575V, 598R, 604G in Nsp2, 
      which were thought to be unique to modified live vaccines (MLVs) or their 
      derivatives. Therefore, they were probably the revertants from MLVs. Our studies 
      showed that the HP-PRRSV strains seemed to be gradually disappearing and 
      NADC30-like strains had become the main causative agents of PRRS in central 
      China. Comparing with HP-PRRSVs, the ORF5 of NADC30-like PRRSV strains displayed 
      extensive amino acid mutations which may be related with immune evasion. 
      Furthermore, the circulation of MLV derivatives in the fields made the diagnosis 
      and control of PRRSV more complicated.
CI  - Copyright (c) 2019 The Authors. Published by Elsevier Ltd.. All rights reserved.
FAU - Guo, Zhenhua
AU  - Guo Z
AD  - Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan 
      Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural 
      Sciences, Zhengzhou, 450002, PR China.
FAU - Chen, Xin-Xin
AU  - Chen XX
AD  - Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan 
      Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural 
      Sciences, Zhengzhou, 450002, PR China.
FAU - Li, Xiang
AU  - Li X
AD  - Department of Veterinary Medicine, Yunnan Agricultural University, Kunming, 
      650100, PR China.
FAU - Qiao, Songlin
AU  - Qiao S
AD  - Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan 
      Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural 
      Sciences, Zhengzhou, 450002, PR China.
FAU - Deng, Ruiguang
AU  - Deng R
AD  - Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan 
      Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural 
      Sciences, Zhengzhou, 450002, PR China.
FAU - Zhang, Gaiping
AU  - Zhang G
AD  - Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan 
      Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural 
      Sciences, Zhengzhou, 450002, PR China; College of Animal Science and Veterinary 
      Medicine, Henan Agricultural University, Zhengzhou, 450002, PR China; Jiangsu 
      Co-innovation Center for Prevention and Control of Important Animal Infectious 
      Diseases and Zoonoses, Yangzhou, 225009, PR China. Electronic address: 
      zhanggaip@126.com.
LA  - eng
PT  - Journal Article
DEP - 20190806
PL  - England
TA  - Microb Pathog
JT  - Microbial pathogenesis
JID - 8606191
RN  - 0 (Epitopes)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
RN  - EC 3.4.22.- (Cysteine Endopeptidases)
RN  - EC 3.4.22.- (nsP2 proteinase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Amino Acid Substitution
MH  - Animals
MH  - China/epidemiology
MH  - Cloning, Molecular
MH  - Cysteine Endopeptidases/genetics
MH  - Epitopes
MH  - *Genetic Variation
MH  - Genome, Viral
MH  - Mutation
MH  - Phylogeny
MH  - Porcine Reproductive and Respiratory Syndrome/*epidemiology/*virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/classification/*genetics/*isolation & purification
MH  - Prevalence
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Swine
MH  - Viral Envelope Proteins/genetics
OTO - NOTNLM
OT  - Epidemiology
OT  - Genetic variation
OT  - NADC30-like strains
OT  - PRRSV
OT  - Revertant
EDAT- 2019/08/10 06:00
MHDA- 2020/01/14 06:00
CRDT- 2019/08/10 06:00
PHST- 2018/07/02 00:00 [received]
PHST- 2019/07/26 00:00 [revised]
PHST- 2019/08/05 00:00 [accepted]
PHST- 2019/08/10 06:00 [pubmed]
PHST- 2020/01/14 06:00 [medline]
PHST- 2019/08/10 06:00 [entrez]
AID - S0882-4010(18)31198-7 [pii]
AID - 10.1016/j.micpath.2019.103657 [doi]
PST - ppublish
SO  - Microb Pathog. 2019 Oct;135:103657. doi: 10.1016/j.micpath.2019.103657. Epub 2019 
      Aug 6.