PMID- 31400126
OWN - NLM
STAT- MEDLINE
DCOM- 20190924
LR  - 20190925
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 36
IP  - 8
DP  - 2019 Aug 10
TI  - [The value of detecting MLL gene rearrangement in children with acute monocytic 
      leukemia].
PG  - 777-780
LID - 10.3760/cma.j.issn.1003-9406.2019.08.006 [doi]
AB  - OBJECTIVE: To assess the value of detecting the rearrangement of mixed lineage 
      leukemia (MLL) gene in children with acute mononuclear leukemia (AML). METHODS: 
      Dual-color fluorescence in situ hybridization (FISH) probe was used to detect MLL 
      gene rearrangement in 68 children with AML by interphase FISH. The results were 
      compared with that of conventional G banding chromosomal analysis. RESULTS: Among 
      the 68 children, 28 were detected by FISH with positive hybridization signals, 
      with a detection rate for MLL gene rearrangement being 41.2%. Twelve (17.6%) 
      reciprocal translocations and interruption of 11q23 were detected by G banding 
      analysis. The difference in the detection rates between the two methods was 
      statistically significant (P< 0.05). CONCLUSION: The sensitivity of FISH assay 
      for MLL gene rearrangement was significantly higher than that of G banding 
      chromosomal karyotyping. Combined use of both methods for children with AML can 
      improve the detection rate of MLL gene rearrangements and provide crucial clues 
      for clinical diagnosis, treatment and prognosis.
FAU - Li, Rui
AU  - Li R
AD  - Genetics Laboratory, Children' s Hospital Affiliated to Zhengzhou University, 
      Henan Children' s Hospital, Zhengzhou Children' s Hospital, Zhengzhou, Henan 
      450018, China. zhaoding2004@163.com.
FAU - Zhao, Ding
AU  - Zhao D
FAU - Wang, Meiye
AU  - Wang M
FAU - Zhang, Zhenhua
AU  - Zhang Z
FAU - Li, Linfei
AU  - Li L
FAU - Wang, Chaojie
AU  - Wang C
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
RN  - 0 (KMT2A protein, human)
RN  - 149025-06-9 (Myeloid-Lymphoid Leukemia Protein)
RN  - EC 2.1.1.43 (Histone-Lysine N-Methyltransferase)
SB  - IM
MH  - Child
MH  - Chromosomes, Human, Pair 11
MH  - Gene Rearrangement
MH  - Histone-Lysine N-Methyltransferase/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Monocytic, Acute/*genetics
MH  - Myeloid-Lymphoid Leukemia Protein/*genetics
MH  - Translocation, Genetic
EDAT- 2019/08/11 06:00
MHDA- 2019/09/26 06:00
CRDT- 2019/08/11 06:00
PHST- 2019/08/11 06:00 [entrez]
PHST- 2019/08/11 06:00 [pubmed]
PHST- 2019/09/26 06:00 [medline]
AID - 940636162 [pii]
AID - 10.3760/cma.j.issn.1003-9406.2019.08.006 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2019 Aug 10;36(8):777-780. doi: 
      10.3760/cma.j.issn.1003-9406.2019.08.006.