PMID- 31400571
OWN - NLM
STAT- MEDLINE
DCOM- 20200625
LR  - 20231020
IS  - 1549-9642 (Electronic)
IS  - 1549-9634 (Print)
IS  - 1549-9634 (Linking)
VI  - 22
DP  - 2019 Nov
TI  - Tethering peptides onto biomimetic and injectable nanofiber microspheres to 
      direct cellular response.
PG  - 102081
LID - S1549-9634(19)30165-0 [pii]
LID - 10.1016/j.nano.2019.102081 [doi]
AB  - Biomimetic and injectable nanofiber microspheres (NMs) could be ideal candidate 
      for minimally invasive tissue repair. Herein, we report a facile approach to 
      fabricate peptide-tethered NMs by combining electrospinning, electrospraying, and 
      surface conjugation techniques. The composition and size of NMs can be tuned by 
      varying the processing parameters during the fabrication. Further, bone 
      morphogenic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) 
      mimicking peptides have been successfully tethered onto poly(epsilon-caprolactone) 
      (PCL):gelatin:(gelatin-methacryloyl) (GelMA)(1:0.5:0.5) NMs through 
      photocrosslinking of the methacrylic group in GelMA and octenyl alanine (OCTAL) 
      in the modified peptides. The BMP-2-OCTAL peptide-tethered NMs significantly 
      promote osteogenic differentiation of bone marrow-derived stem cells (BMSCs). 
      Moreover, human umbilical vein endothelial cells (HUVECs) seeded on VEGF 
      mimicking peptide QK-OCTAL-tethered NMs significantly up-regulated 
      vascular-specific proteins, leading to microvascularization. The strategy 
      developed in this work holds great potential in developing a biomimetic and 
      injectable carrier to efficiently direct cellular response (Osteogenesis and 
      Angiogenesis) for tissue repair.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - John, Johnson V
AU  - John JV
AD  - Department of Surgery-Transplant and Mary & Dick Holland Regenerative Medicine 
      Program, College of Medicine, University of Nebraska Medical Center, Omaha, NE, 
      USA.
FAU - Choksi, Meera
AU  - Choksi M
AD  - Department of Surgery-Transplant and Mary & Dick Holland Regenerative Medicine 
      Program, College of Medicine, University of Nebraska Medical Center, Omaha, NE, 
      USA.
FAU - Chen, Shixuan
AU  - Chen S
AD  - Department of Surgery-Transplant and Mary & Dick Holland Regenerative Medicine 
      Program, College of Medicine, University of Nebraska Medical Center, Omaha, NE, 
      USA.
FAU - Boda, Sunil Kumar
AU  - Boda SK
AD  - Department of Surgery-Transplant and Mary & Dick Holland Regenerative Medicine 
      Program, College of Medicine, University of Nebraska Medical Center, Omaha, NE, 
      USA.
FAU - Su, Yajuan
AU  - Su Y
AD  - Department of Surgery-Transplant and Mary & Dick Holland Regenerative Medicine 
      Program, College of Medicine, University of Nebraska Medical Center, Omaha, NE, 
      USA.
FAU - McCarthy, Alec
AU  - McCarthy A
AD  - Department of Surgery-Transplant and Mary & Dick Holland Regenerative Medicine 
      Program, College of Medicine, University of Nebraska Medical Center, Omaha, NE, 
      USA.
FAU - Teusink, Matthew J
AU  - Teusink MJ
AD  - Department of Orthopaedic Surgery and Rehabilitation, College of Medicine, 
      University of Nebraska Medical Center, Omaha, NE, USA.
FAU - Reinhardt, Richard A
AU  - Reinhardt RA
AD  - Department of Surgical Specialties, College of Dentistry, University of Nebraska 
      Medical Center, Omaha, NE, USA.
FAU - Xie, Jingwei
AU  - Xie J
AD  - Department of Surgery-Transplant and Mary & Dick Holland Regenerative Medicine 
      Program, College of Medicine, University of Nebraska Medical Center, Omaha, NE, 
      USA; Department of Mechanical and Materials Engineering, College of Engineering, 
      University of Nebraska Lincoln, Lincoln, NE, USA. Electronic address: 
      jingwei.xie@unmc.edu.
LA  - eng
GR  - P20 GM103480/GM/NIGMS NIH HHS/United States
GR  - P30 GM127200/GM/NIGMS NIH HHS/United States
GR  - R21 DE027516/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20190807
PL  - United States
TA  - Nanomedicine
JT  - Nanomedicine : nanotechnology, biology, and medicine
JID - 101233142
RN  - 0 (Bone Morphogenetic Protein 2)
RN  - 0 (Peptides)
RN  - 0 (Polyesters)
RN  - 0 (RNA, Messenger)
RN  - 106441-73-0 (Osteopontin)
RN  - 24980-41-4 (polycaprolactone)
RN  - 9000-70-8 (Gelatin)
SB  - IM
MH  - Animals
MH  - Biomimetic Materials/*pharmacology
MH  - Bone Morphogenetic Protein 2/pharmacology
MH  - Cell Differentiation/drug effects/genetics
MH  - Gelatin/pharmacology
MH  - Human Umbilical Vein Endothelial Cells/drug effects
MH  - Humans
MH  - *Injections
MH  - Kinetics
MH  - Light
MH  - Mesenchymal Stem Cells/*cytology/drug effects
MH  - *Microspheres
MH  - Microvessels/drug effects/metabolism
MH  - Nanofibers/*chemistry/ultrastructure
MH  - Neovascularization, Physiologic/drug effects
MH  - Osteogenesis/drug effects/genetics
MH  - Osteopontin/metabolism
MH  - Peptides/*pharmacology
MH  - Polyesters/pharmacology
MH  - RNA, Messenger/genetics/metabolism
MH  - Tissue Engineering
PMC - PMC6904511
MID - NIHMS1536827
OTO - NOTNLM
OT  - Cellular response
OT  - Microvascularization
OT  - Nanofiber microspheres
OT  - Osteogenic differentiation
OT  - Peptides conjugation
COIS- Conflict of Interest: None.
EDAT- 2019/08/11 06:00
MHDA- 2020/06/26 06:00
PMCR- 2020/11/01
CRDT- 2019/08/11 06:00
PHST- 2019/05/15 00:00 [received]
PHST- 2019/07/30 00:00 [revised]
PHST- 2019/07/31 00:00 [accepted]
PHST- 2019/08/11 06:00 [pubmed]
PHST- 2020/06/26 06:00 [medline]
PHST- 2019/08/11 06:00 [entrez]
PHST- 2020/11/01 00:00 [pmc-release]
AID - S1549-9634(19)30165-0 [pii]
AID - 10.1016/j.nano.2019.102081 [doi]
PST - ppublish
SO  - Nanomedicine. 2019 Nov;22:102081. doi: 10.1016/j.nano.2019.102081. Epub 2019 Aug 
      7.