PMID- 31401234
OWN - NLM
STAT- MEDLINE
DCOM- 20200610
LR  - 20200610
IS  - 1532-8392 (Electronic)
IS  - 0046-8177 (Linking)
VI  - 92
DP  - 2019 Oct
TI  - The expression of gastrointestinal differentiation markers in extrahepatic 
      cholangiocarcinoma: clinicopathological significance based on tumor location.
PG  - 91-100
LID - S0046-8177(19)30135-2 [pii]
LID - 10.1016/j.humpath.2019.08.002 [doi]
AB  - The expression of gastrointestinal differentiation markers is associated with the 
      tumorigenesis and prognosis of digestive cancers. However, little is known about 
      the significance of gastrointestinal differentiation marker profiles in patients 
      with extrahepatic cholangiocarcinoma (CCA), which is classified as perihilar and 
      distal CCA. The purpose of this study was to clarify the role of gastrointestinal 
      differentiation marker expression in extrahepatic CCA based on tumor location. We 
      examined the expression of gastrointestinal differentiation markers in resected 
      perihilar (n = 30) and distal (n = 54) CCAs based on the immunohistochemical 
      expression of the following markers: MUC2, MUC5AC, MUC6, CD10, CDX-2, and 
      cytokeratin 20. Expression scores were determined semiquantitatively based on the 
      rate of positively stained cells. Furthermore, we performed hierarchical 
      clustering of the CCAs based on the immunohistochemical expression scores to 
      evaluate differences in the expression patterns of the 6 gastrointestinal 
      differentiation markers. Consequently, perihilar and distal CCAs were stratified 
      into 2 subgroups each. Among the perihilar CCAs, subgroup 1 was characterized by 
      lower expression of MUC5AC and MUC6, a larger median tumor size, and a 
      significantly worse prognosis compared with subgroup 2. Furthermore, the 
      immunohistological subgroup (subgroup 1 versus 2) and TNM stage (stage III versus 
      II) were independent predictors of patient survival. Among the distal CCAs, 
      subgroup 1 was characterized by lower expression of MUC5AC compared with subgroup 
      2. We suggest that gastrointestinal differentiation marker profiles are useful 
      for stratifying perihilar and distal CCAs. In addition, gastrointestinal 
      differentiation markers play a crucial role in tumor development, particularly in 
      perihilar CCA.
CI  - Copyright (c) 2019 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Ishida, Kazuyuki
AU  - Ishida K
AD  - Department of Molecular Diagnostic Pathology, Iwate Medical University, Morioka, 
      Iwate 020-8505, Japan.
FAU - Osakabe, Mitsumasa
AU  - Osakabe M
AD  - Department of Molecular Diagnostic Pathology, Iwate Medical University, Morioka, 
      Iwate 020-8505, Japan.
FAU - Eizuka, Makoto
AU  - Eizuka M
AD  - Department of Molecular Diagnostic Pathology, Iwate Medical University, Morioka, 
      Iwate 020-8505, Japan.
FAU - Tai, Seigo
AU  - Tai S
AD  - Department of Molecular Diagnostic Pathology, Iwate Medical University, Morioka, 
      Iwate 020-8505, Japan.
FAU - Sugimoto, Ryo
AU  - Sugimoto R
AD  - Department of Molecular Diagnostic Pathology, Iwate Medical University, Morioka, 
      Iwate 020-8505, Japan.
FAU - Fujita, Yasuko
AU  - Fujita Y
AD  - Department of Molecular Diagnostic Pathology, Iwate Medical University, Morioka, 
      Iwate 020-8505, Japan.
FAU - Katagiri, Hirokatsu
AU  - Katagiri H
AD  - Department of Surgery, Iwate Medical University, Morioka, Iwate 020-8505, Japan.
FAU - Takahara, Takeshi
AU  - Takahara T
AD  - Department of Surgery, Iwate Medical University, Morioka, Iwate 020-8505, Japan.
FAU - Uesugi, Noriyuki
AU  - Uesugi N
AD  - Department of Molecular Diagnostic Pathology, Iwate Medical University, Morioka, 
      Iwate 020-8505, Japan.
FAU - Nitta, Hiroyuki
AU  - Nitta H
AD  - Department of Surgery, Iwate Medical University, Morioka, Iwate 020-8505, Japan.
FAU - Sasaki, Akira
AU  - Sasaki A
AD  - Department of Surgery, Iwate Medical University, Morioka, Iwate 020-8505, Japan.
FAU - Sugai, Tamotsu
AU  - Sugai T
AD  - Department of Molecular Diagnostic Pathology, Iwate Medical University, Morioka, 
      Iwate 020-8505, Japan. Electronic address: tsugai@iwate-med.ac.jp.
LA  - eng
PT  - Journal Article
DEP - 20190808
PL  - United States
TA  - Hum Pathol
JT  - Human pathology
JID - 9421547
RN  - 0 (CDX2 Transcription Factor)
RN  - 0 (CDX2 protein, human)
RN  - 0 (Keratin-20)
RN  - 0 (Mucins)
RN  - EC 3.4.24.11 (Neprilysin)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Bile Duct Neoplasms/*metabolism/pathology
MH  - CDX2 Transcription Factor/*metabolism
MH  - Cholangiocarcinoma/*metabolism/pathology
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Keratin-20/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Mucins/*metabolism
MH  - Neprilysin/*metabolism
OTO - NOTNLM
OT  - Distal cholangiocarcinoma
OT  - Gastrointestinal differentiation marker
OT  - Immunohistochemistry
OT  - Patient survival
OT  - Perihilar cholangiocarcinoma
EDAT- 2019/08/12 06:00
MHDA- 2020/06/11 06:00
CRDT- 2019/08/12 06:00
PHST- 2019/04/15 00:00 [received]
PHST- 2019/07/28 00:00 [revised]
PHST- 2019/08/01 00:00 [accepted]
PHST- 2019/08/12 06:00 [pubmed]
PHST- 2020/06/11 06:00 [medline]
PHST- 2019/08/12 06:00 [entrez]
AID - S0046-8177(19)30135-2 [pii]
AID - 10.1016/j.humpath.2019.08.002 [doi]
PST - ppublish
SO  - Hum Pathol. 2019 Oct;92:91-100. doi: 10.1016/j.humpath.2019.08.002. Epub 2019 Aug 
      8.