PMID- 31401453
OWN - NLM
STAT- MEDLINE
DCOM- 20191230
LR  - 20191230
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 1126-1127
DP  - 2019 Sep 15
TI  - Development a simple and rapid HPLC-ESI-Q-TOF/MS method for determination of 
      short- and medium- chain chlorinated paraffins in human serum.
PG  - 121722
LID - S1570-0232(19)30522-7 [pii]
LID - 10.1016/j.jchromb.2019.121722 [doi]
AB  - This study established a simple and rapid method for simultaneous determination 
      of short-chain chlorinated paraffins (SCCPs) and medium-chain chlorinated 
      paraffins (MCCPs) in human serum by high performance liquid chromatography 
      coupled with electron spray ionization quadrupole time-of-flight mass 
      spectrometry (HPLC-ESI-Q-TOF/MS). A simple pretreatment procedure of protein 
      precipitation by acetonitrile coupled with liquid-liquid extraction by n-hexane 
      was employed for extraction and purification. The purified samples were separated 
      by a PFP chromatographic column. This method could effectively eliminate the 
      matrix effect by lipids and other matrix substances in serum samples with less 
      time and solvent consuming. 76 congener groups of SCCPs and MCCPs with 
      C(10)-C(17) and Cl(5)-Cl(13) were quantified by their [M-H](-) ions. Method 
      detection limit (MDL) were 1.0-8.0 ng mL(-1) for  summation operatorSCCPs and  summation operatorMCCPs. Recoveries 
      were 98.4 +/- 4.42%, 98.8 +/- 3.96% and 90.7 +/- 2.79% for SCCPs and 93.1 +/- 6.67%, 
      108 +/- 1.21% and 90.8 +/- 3.78% for MCCPs at spiking concentrations of 20, 50 and 
      200 ng.mL(-1), respectively. The relative standard deviations (RSDs) of intra- 
      and inter-day variations were 1.41% and 9.84% for SCCPs, and 4.23% and 6.26% for 
      MCCPs. The suitability of the developed method was demonstrated through the 
      application to analysis of SCCPs and MCCPs in human serum samples.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Zhou, Xi
AU  - Zhou X
AD  - Guangdong Provincial Key Laboratory of Emergency Test for Dangerous Chemicals, 
      Guangdong Provincial Public Laboratory of Analysis and Testing Technology, 
      Guangdong Institute of Analysis, Guangzhou 510070, Guangdong, China.
FAU - Wu, Huiqin
AU  - Wu H
AD  - Guangdong Provincial Key Laboratory of Emergency Test for Dangerous Chemicals, 
      Guangdong Provincial Public Laboratory of Analysis and Testing Technology, 
      Guangdong Institute of Analysis, Guangzhou 510070, Guangdong, China. Electronic 
      address: 13802959756@139.com.
FAU - Huang, Xiaolan
AU  - Huang X
AD  - Guangdong Provincial Key Laboratory of Emergency Test for Dangerous Chemicals, 
      Guangdong Provincial Public Laboratory of Analysis and Testing Technology, 
      Guangdong Institute of Analysis, Guangzhou 510070, Guangdong, China.
FAU - Hang, Fang
AU  - Hang F
AD  - Guangdong Provincial Key Laboratory of Emergency Test for Dangerous Chemicals, 
      Guangdong Provincial Public Laboratory of Analysis and Testing Technology, 
      Guangdong Institute of Analysis, Guangzhou 510070, Guangdong, China.
FAU - Luo, Huitai
AU  - Luo H
AD  - Guangdong Provincial Key Laboratory of Emergency Test for Dangerous Chemicals, 
      Guangdong Provincial Public Laboratory of Analysis and Testing Technology, 
      Guangdong Institute of Analysis, Guangzhou 510070, Guangdong, China.
LA  - eng
PT  - Journal Article
DEP - 20190718
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Hydrocarbons, Chlorinated)
RN  - 8002-74-2 (Paraffin)
SB  - IM
MH  - Adult
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Humans
MH  - *Hydrocarbons, Chlorinated/blood/chemistry
MH  - Limit of Detection
MH  - Linear Models
MH  - Middle Aged
MH  - *Paraffin/analogs & derivatives/analysis/chemistry
MH  - Reproducibility of Results
MH  - Young Adult
OTO - NOTNLM
OT  - High performance liquid chromatography coupled with electron spray ionization 
      quadrupole time-of-flight mass spectrometry
OT  - Human serum
OT  - Medium-chain chlorinated paraffins
OT  - Short-chain chlorinated paraffins
EDAT- 2019/08/12 06:00
MHDA- 2019/12/31 06:00
CRDT- 2019/08/12 06:00
PHST- 2019/03/28 00:00 [received]
PHST- 2019/07/04 00:00 [revised]
PHST- 2019/07/17 00:00 [accepted]
PHST- 2019/08/12 06:00 [pubmed]
PHST- 2019/12/31 06:00 [medline]
PHST- 2019/08/12 06:00 [entrez]
AID - S1570-0232(19)30522-7 [pii]
AID - 10.1016/j.jchromb.2019.121722 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2019 Sep 15;1126-1127:121722. doi: 
      10.1016/j.jchromb.2019.121722. Epub 2019 Jul 18.