PMID- 31401655
OWN - NLM
STAT- MEDLINE
DCOM- 20200409
LR  - 20200409
IS  - 1615-7605 (Electronic)
IS  - 1615-7591 (Linking)
VI  - 42
IP  - 12
DP  - 2019 Dec
TI  - Modulation of fungal biofilm physiology and secondary product formation based on 
      physico-chemical surface properties.
PG  - 1935-1946
LID - 10.1007/s00449-019-02187-6 [doi]
AB  - Relative to the amount of knowledge concerning bacterial biofilms, little is 
      known about the impact of physico-chemical properties of support material on 
      fungal biofilm adhesion and physiology. In the field of industrial fermentation, 
      large-scale production of low-cost fungal secondary product is a challenging area 
      of research. In the present work, the effect of physico-chemical surface 
      properties of five different materials (Teflon, glass, Viton rubber, silicon 
      rubber, and stainless steel) on the production of class II hydrophobins (HFBI and 
      HFBII) from Trichoderma reesei (HFB2a-2) and Trichoderma harzianum) was 
      evaluated. Two culture systems (shake flask and drip flow reactor (DFR)) were 
      used in this study to promote biomass growth and the production of hydrophobins. 
      Furthermore, the effect of physico-chemical surface properties (hydrophobicity, 
      surface energy) and surface texture (roughness) of support material on the 
      initial colonization and attachment of the fungal biofilm was evaluated. Maximum 
      biofilm productivity was obtained using Viton rubber for T. reesei and Viton 
      rubber and stainless steel as support materials for T. harzianum. Scanning 
      electron microscope (SEM) revealed that fungal biofilm adhesion was higher on the 
      rough hydrophobic Viton rubber surface as compared to the smooth hydrophobic 
      Teflon surface. Initial colonization initiated because of surface irregularities 
      and holes in the material as hyphal filaments. Moreover, compared to traditional 
      submerged fermentation, a significant increase in biofilm productivity for both 
      strains (T. reesei, T. harzianum) in all five materials was obtained.
FAU - Bajoul Kakahi, F
AU  - Bajoul Kakahi F
AD  - Department of Microbial and Molecular Systems (M2S), KULeuven, 3001, Heverlee, 
      Belgium.
FAU - Ly, S
AU  - Ly S
AUID- ORCID: 0000-0002-9895-3007
AD  - Gembloux Agro-Bio tech, TERRA Research and Teaching Center, Microbial Processes 
      and Interactions, University of Liege, Avenue de la Faculte 2B, 5030, Gembloux, 
      Belgium.
AD  - Faculty of Chemical and Food Engineering, Institute of Technology of Cambodia, 
      Phnom Penh, 12156, Cambodia.
FAU - Tarayre, C
AU  - Tarayre C
AD  - Gembloux Agro-Bio tech, TERRA Research and Teaching Center, Microbial Processes 
      and Interactions, University of Liege, Avenue de la Faculte 2B, 5030, Gembloux, 
      Belgium.
FAU - Deschaume, O
AU  - Deschaume O
AD  - Soft-Matter Physics and Biophysics Section, Department of Physics and Astronomy, 
      KU Leuven, Celestijnenlaan 200 D, Box 2416, 3001, Heverlee, Belgium.
FAU - Bartic, C
AU  - Bartic C
AD  - Soft-Matter Physics and Biophysics Section, Department of Physics and Astronomy, 
      KU Leuven, Celestijnenlaan 200 D, Box 2416, 3001, Heverlee, Belgium.
FAU - Wagner, P
AU  - Wagner P
AD  - Soft-Matter Physics and Biophysics Section, Department of Physics and Astronomy, 
      KU Leuven, Celestijnenlaan 200 D, Box 2416, 3001, Heverlee, Belgium.
FAU - Compere, P
AU  - Compere P
AD  - Department of Biology, Ecology, Evolution, Freshwater and Oceanic Science Unit of 
      Research FOCUS & Centre of Aid for Research and Teaching in Microscopy (CAREM), 
      University of Liege, Campus du Sart-Tilman, Allee de six Aout 15, 4000, Liege 1, 
      Belgium.
FAU - Derdelinckx, G
AU  - Derdelinckx G
AD  - Department of Microbial and Molecular Systems (M2S), KULeuven, 3001, Heverlee, 
      Belgium.
FAU - Blecker, C
AU  - Blecker C
AD  - Department of Food Technology, Gembloux Agro-Bio Tech University of Liege, Avenue 
      de la Faculte 2B, 5030, Gembloux, Belgium.
FAU - Delvigne, F
AU  - Delvigne F
AD  - Gembloux Agro-Bio tech, TERRA Research and Teaching Center, Microbial Processes 
      and Interactions, University of Liege, Avenue de la Faculte 2B, 5030, Gembloux, 
      Belgium. f.delvigne@uliege.be.
LA  - eng
PT  - Journal Article
DEP - 20190810
PL  - Germany
TA  - Bioprocess Biosyst Eng
JT  - Bioprocess and biosystems engineering
JID - 101088505
RN  - 0 (Fungal Proteins)
RN  - 9002-84-0 (Polytetrafluoroethylene)
SB  - IM
MH  - *Biofilms
MH  - Biomass
MH  - Bioreactors
MH  - Fermentation
MH  - Fungal Proteins/chemistry
MH  - Fungi/*physiology
MH  - Hydrophobic and Hydrophilic Interactions
MH  - Microscopy, Atomic Force
MH  - Microscopy, Electron, Scanning
MH  - Polytetrafluoroethylene
MH  - Surface Properties
MH  - Trichoderma/*physiology
OTO - NOTNLM
OT  - Biofilm reactor
OT  - Hydrophobin
OT  - Solid-state physiology
OT  - Surface colonization
OT  - Trichoderma
EDAT- 2019/08/12 06:00
MHDA- 2020/04/10 06:00
CRDT- 2019/08/12 06:00
PHST- 2019/06/24 00:00 [received]
PHST- 2019/08/01 00:00 [accepted]
PHST- 2019/07/24 00:00 [revised]
PHST- 2019/08/12 06:00 [pubmed]
PHST- 2020/04/10 06:00 [medline]
PHST- 2019/08/12 06:00 [entrez]
AID - 10.1007/s00449-019-02187-6 [pii]
AID - 10.1007/s00449-019-02187-6 [doi]
PST - ppublish
SO  - Bioprocess Biosyst Eng. 2019 Dec;42(12):1935-1946. doi: 
      10.1007/s00449-019-02187-6. Epub 2019 Aug 10.