PMID- 31401730
OWN - NLM
STAT- MEDLINE
DCOM- 20200424
LR  - 20200424
IS  - 0975-0711 (Electronic)
IS  - 0254-8860 (Linking)
VI  - 38
IP  - 4
DP  - 2019 Aug
TI  - Could quantitative real-time polymerase chain reaction assay serve as an 
      alternative test method to evaluate human epidermal growth factor receptor 2 
      status of gastric carcinoma in the South Asian setting?
PG  - 317-324
LID - 10.1007/s12664-019-00955-6 [doi]
AB  - BACKGROUND: Human epidermal growth factor receptor 2 (HER2) protein 
      overexpression and/or HER2 gene amplification are/is linked to a dismal outcome 
      of gastric carcinoma (GCa). Immunohistochemistry (IHC) and fluorescence in situ 
      hybridization (FISH) are key methods to identify patients for HER2 targeted 
      therapy. Drawbacks of both the methods warrant novel tests. Hence, we evaluated 
      the value of quantitative real-time polymerase chain reaction (qPCR) as an 
      alternative test method, relative to IHC to detect HER2 status of GCa and to find 
      relationship between these  results with demographic/clinicopathological data. 
      METHOD: Twenty GCa patients with known IHC HER2 scores were evaluated. qPCR was 
      performed for the HER2 gene and amyloid precursor protein (reference gene) in 
      formalin-fixed paraffin-embedded GCa tissue. Cycle threshold values (Ct) were 
      analyzed using the Pfaffl method to detect HER2 gene amplification. RESULTS: HER2 
      positivity rates by IHC and qPCR were 20% and 35%, respectively. The sensitivity 
      and specificity of qPCR were 67% and 76%, respectively, relative to IHC. qPCR 
      results were reproducible. The diagnostic consistency between IHC and qPCR 
      (kappa = 0.146) was slightly agreeable (0.01 < k < 0.20), with a 65% concordance. 
      Based on McNemar's test, there was no significant difference between the results 
      of the two tests. IHC HER2 protein expression had relationship with the tumor 
      (TNM) stage and Lauren histological type (p < 0.05). Positive HER2 gene 
      expression by qPCR showed relationship with depth of invasion, lymph node 
      involvement, and degree of differentiation (p < 0.05). CONCLUSION: Cost-effective 
      qPCR could serve as an alternative test method for detection of HER2 status of 
      GCa. Both HER2 overexpression by IHC and gene amplification by qPCR are 
      associated with adverse clinicopathological features.
FAU - Kannangara, D K S
AU  - Kannangara DKS
AD  - Post Graduate Institute of Medicine, University of Colombo, Colombo, Sri Lanka.
FAU - Lokuhetty, M D S
AU  - Lokuhetty MDS
AD  - Department of Pathology, Faculty of Medicine, University of Colombo, Colombo, Sri 
      Lanka.
FAU - Subasinghe, D
AU  - Subasinghe D
AD  - Department of Surgery, Faculty of Medicine, The National Hospital of Sri Lanka, 
      University of Colombo/University Surgical Unit, Colombo, Sri Lanka.
FAU - Gunawardene, Y I N S
AU  - Gunawardene YINS
AD  - Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Colombo, 
      Sri Lanka.
FAU - Dassanayake, R S
AU  - Dassanayake RS
AD  - Department of Chemistry, Faculty of Science, University of Colombo, Colombo, Sri 
      Lanka. rsdassanayake@chem.cmb.ac.lk.
LA  - eng
GR  - AP/3/2011/CG/03/University of Colombo/International
PT  - Comparative Study
PT  - Journal Article
DEP - 20190810
PL  - India
TA  - Indian J Gastroenterol
JT  - Indian journal of gastroenterology : official journal of the Indian Society of 
      Gastroenterology
JID - 8409436
RN  - 0 (Biomarkers, Tumor)
RN  - EC 2.7.10.1 (ERBB2 protein, human)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Adult
MH  - Biomarkers, Tumor/analysis
MH  - Carcinoma/*genetics
MH  - Cross-Sectional Studies
MH  - Female
MH  - Gene Amplification
MH  - Humans
MH  - Immunohistochemistry/methods/*statistics & numerical data
MH  - Male
MH  - Real-Time Polymerase Chain Reaction/methods/*statistics & numerical data
MH  - Receptor, ErbB-2/*analysis
MH  - Sensitivity and Specificity
MH  - Sri Lanka
MH  - Stomach Neoplasms/*genetics
OTO - NOTNLM
OT  - Gastric carcinoma
OT  - HER2 status
OT  - Quantitative PCR assay
EDAT- 2019/08/12 06:00
MHDA- 2020/04/25 06:00
CRDT- 2019/08/12 06:00
PHST- 2018/09/20 00:00 [received]
PHST- 2019/04/14 00:00 [accepted]
PHST- 2019/08/12 06:00 [pubmed]
PHST- 2020/04/25 06:00 [medline]
PHST- 2019/08/12 06:00 [entrez]
AID - 10.1007/s12664-019-00955-6 [pii]
AID - 10.1007/s12664-019-00955-6 [doi]
PST - ppublish
SO  - Indian J Gastroenterol. 2019 Aug;38(4):317-324. doi: 10.1007/s12664-019-00955-6. 
      Epub 2019 Aug 10.