PMID- 31402954
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 18
IP  - 3
DP  - 2019 Sep
TI  - miR-296-3p targets APEX1 to suppress cell migration and invasion of 
      non-small-cell lung cancer.
PG  - 2612-2618
LID - 10.3892/ol.2019.10572 [doi]
AB  - Non-small-cell lung cancer (NSCLC) is the most common cause of cancer-associated 
      mortality worldwide. MicroRNAs (miRs) are a class of small non-coding RNAs that 
      are commonly dysregulated in human cancer. The aim of the current study was to 
      evaluate the effect of miR-296-3p on the cell migration and invasion of NSCLC. 
      Pairs of tumor tissues and para-cancerous tissues (n=50) were collected from 
      patients with NSCLC, and the expression of miR-296-3p was analyzed by reverse 
      transcription-quantitative polymerase chain reaction (RT-qPCR). Additionally, 
      tumor cell viability, migration and invasion were examined in vitro using Cell 
      Counting Kit-8, wound healing and Matrigel assays, respectively. Furthermore, 
      potential targets of miR-296-3p were screened for using TargetScan and validated 
      using a dual-luciferase reporter assay. The expression levels of 
      phosphoinositide-3-kinase (PI3K), AKT serine/threonine kinase (AKT), mammalian 
      target of rapamycin (mTOR), matrix metallopeptidase 2 (MMP2) and SRY-box 4 (SOX4) 
      were detected by RT-qPCR and western blot analysis. The data indicated that 
      miR-296-3p was downregulated in tumor tissues compared with adjacent normal 
      tissues. Overexpression of miR-296-3p inhibited NSCLC cell viability, migration 
      and invasion in vitro. Furthermore, apurinic/apyrimidinic endodeoxyribonuclease 1 
      (APEX1) was identified as a direct target of miR-296-3p. APEX1 expression was 
      upregulated in tumor tissues compared with para-cancerous tissues, and the mRNA 
      and protein expression levels of APEX1 were decreased following transfection of 
      NSCLC cells with miR-296-3p mimics compared with control cells. Additional 
      investigations revealed that miR-296-3p was involved in regulating the 
      PI3K/AKT/mTOR signaling pathway, and miR-296-3p mimics decreased the mRNA and 
      protein expression levels of MMP2 and SOX4. In summary, the findings demonstrated 
      that miR-296-3p may function as a tumor suppressor, and inhibits the migration 
      and invasion of NSCLC cells by targeting APEX1. miR-296-3p is therefore a 
      potential therapeutic molecular modulator of NSCLC.
FAU - Wang, Lifeng
AU  - Wang L
AD  - Department of Respiration, Xi'an High-tech Hospital, Xi'an, Shaanxi 710075, P.R. 
      China.
FAU - Chen, Ruilin
AU  - Chen R
AD  - Department of Respiration, Shaanxi Provincial People's Hospital, Xi'an, Shaanxi 
      710068, P.R. China.
FAU - Zhang, Yongqing
AU  - Zhang Y
AD  - Department of Respiration, Shaanxi Provincial People's Hospital, Xi'an, Shaanxi 
      710068, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20190705
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC6676713
OTO - NOTNLM
OT  - apurinic/apyrimidinic endodeoxyribonuclease 1
OT  - invasion
OT  - microRNA-296-3p
OT  - migration
OT  - non-small-cell lung cancer
EDAT- 2019/08/14 06:00
MHDA- 2019/08/14 06:01
PMCR- 2019/07/05
CRDT- 2019/08/13 06:00
PHST- 2018/07/30 00:00 [received]
PHST- 2019/05/03 00:00 [accepted]
PHST- 2019/08/13 06:00 [entrez]
PHST- 2019/08/14 06:00 [pubmed]
PHST- 2019/08/14 06:01 [medline]
PHST- 2019/07/05 00:00 [pmc-release]
AID - OL-0-0-10572 [pii]
AID - 10.3892/ol.2019.10572 [doi]
PST - ppublish
SO  - Oncol Lett. 2019 Sep;18(3):2612-2618. doi: 10.3892/ol.2019.10572. Epub 2019 Jul 
      5.