PMID- 31403678
OWN - NLM
STAT- MEDLINE
DCOM- 20210609
LR  - 20211204
IS  - 1529-7268 (Electronic)
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 102
IP  - 1
DP  - 2020 Feb 12
TI  - Expression and functional analyses of ephrin type-A receptor 2 in mouse 
      spermatogonial stem cellsdagger.
PG  - 220-232
LID - 10.1093/biolre/ioz156 [doi]
AB  - Spermatogonial stem cells (SSCs) undergo continuous self-renewal division in 
      response to self-renewal factors. The present study identified ephrin type-A 
      receptor 2 (EPHA2) on mouse SSCs and showed that supplementation of glial 
      cell-derived neurotrophic factor (GDNF) and fibroblast growth factor 2 (FGF2), 
      which are both SSC self-renewal factors, induced EPHA2 expression in cultured 
      SSCs. Spermatogonial transplantation combined with magnetic-activated cell 
      sorting or fluorescence-activated cell sorting also revealed that EPHA2 was 
      expressed in SSCs. Additionally, ret proto-oncogene (RET) phosphorylation levels 
      decreased following the knockdown (KD) of Epha2 expression via short hairpin 
      ribonucleic acid (RNA). Although the present immunoprecipitation experiments did 
      not reveal an association between RET with EPHA2, RET interacted with FGFR2. The 
      Epha2 KD decreased the proliferation of cultured SSCs and inhibited the binding 
      of cultured SSCs to laminin-coated plates. The Epha2 KD also significantly 
      reduced the colonization of testis cells by spermatogonial transplantation. EPHA2 
      was also expressed in human GDNF family receptor alpha 1-positive spermatogonia. 
      The present results indicate that SSCs express EPHA2 and suggest that it is a 
      critical modifier of self-renewal signals in SSCs.
CI  - (c) The Author(s) 2019. Published by Oxford University Press on behalf of Society 
      for the Study of Reproduction. All rights reserved. For permissions, please 
      e-mail: journals.permissions@oup.com.
FAU - Morimoto, Hiroko
AU  - Morimoto H
AD  - Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, 
      Kyoto, Japan.
FAU - Kanatsu-Shinohara, Mito
AU  - Kanatsu-Shinohara M
AD  - Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, 
      Kyoto, Japan.
AD  - Agency for Medical Research and Development-Core Research for Evolutional Science 
      and Technology, Tokyo, Japan.
FAU - Orwig, Kyle E
AU  - Orwig KE
AD  - Department of Obstetrics, Gynecology and Reproductive Sciences, School of 
      Medicine, Magee-Womens Research Institute, University of Pittsburgh, Pittsburgh, 
      Pennsylvania, USA.
FAU - Shinohara, Takashi
AU  - Shinohara T
AD  - Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, 
      Kyoto, Japan.
LA  - eng
GR  - R01 HD092084/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
RN  - 0 (MAS1 protein, human)
RN  - 0 (Proto-Oncogene Mas)
RN  - 0 (RNA, Small Interfering)
RN  - EC 2.7.10.1 (Receptors, Eph Family)
SB  - IM
MH  - Adult Germline Stem Cells/cytology/*metabolism
MH  - Animals
MH  - Cell Proliferation/physiology
MH  - Glial Cell Line-Derived Neurotrophic Factor/metabolism
MH  - Male
MH  - Mice
MH  - Phosphorylation
MH  - Proto-Oncogene Mas
MH  - RNA, Small Interfering
MH  - Receptors, Eph Family/genetics/*metabolism
MH  - Spermatogonia/cytology/*metabolism
MH  - Testis/*metabolism
PMC - PMC7305687
OTO - NOTNLM
OT  - Epha2
OT  - FGF2
OT  - GDNF
OT  - spermatogenesis
OT  - spermatogonia
EDAT- 2019/08/14 06:00
MHDA- 2021/06/10 06:00
PMCR- 2021/02/12
CRDT- 2019/08/13 06:00
PHST- 2019/04/05 00:00 [received]
PHST- 2019/07/06 00:00 [revised]
PHST- 2019/08/02 00:00 [accepted]
PHST- 2019/08/14 06:00 [pubmed]
PHST- 2021/06/10 06:00 [medline]
PHST- 2019/08/13 06:00 [entrez]
PHST- 2021/02/12 00:00 [pmc-release]
AID - 5546742 [pii]
AID - ioz156 [pii]
AID - 10.1093/biolre/ioz156 [doi]
PST - ppublish
SO  - Biol Reprod. 2020 Feb 12;102(1):220-232. doi: 10.1093/biolre/ioz156.