PMID- 31404337
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231013
IS  - 2056-3418 (Print)
IS  - 2056-3426 (Electronic)
IS  - 2056-3426 (Linking)
VI  - 6
IP  - 4
DP  - 2019 Aug
TI  - Accelerated mineralization on nanofibers via non-thermal atmospheric plasma 
      assisted glutamic acid templated peptide conjugation.
PG  - 231-240
LID - 10.1093/rb/rbz014 [doi]
AB  - Surface modification by non-thermal atmospheric plasma (NTAP) treatment can 
      produce significantly higher carboxylic groups on the nanofibers (NF) surface, 
      which potentially can increase biomineralization of NF via promoting glutamic 
      acid (GLU) templated peptide conjugation. Herein, electrospun 
      poly(lactide-co-glycolide) (PLGA) scaffolds were treated with NTAP and conjugated 
      with GLU peptide followed by incubation in simulated body fluids for 
      mineralization. The effect of NTAP treatment and GLU peptide conjugation on 
      mineralization, surface wettability and roughness were investigated. The results 
      showed that NTAP treatment significantly increased GLU peptide conjugation which 
      consequently enhanced mineralization and mechanical properties of NTAP treated 
      and peptide conjugated NF (GLU-pNF) compared to neat PLGA NF, NTAP treated NF 
      (pNF) and GLU peptide conjugated NF (GLU-NF). The effect of surface modification 
      on human bone marrow derived mesenchymal stem cells adhesion, proliferation and 
      morphology was evaluated by cell proliferation assay and fluorescent microscopy. 
      Results demonstrated that cellular adhesion and proliferation were significantly 
      higher on GLU-pNF compared to NF, pNF and GLU-NF. In summary, NTAP treatment 
      could be a promising modification technique to induce biomimetic peptide 
      conjugation and biomineralization for bone tissue engineering applications.
FAU - Onak, Gunnur
AU  - Onak G
AD  - Tissue Engineering and Regenerative Medicine Laboratory, Department of Biomedical 
      Engineering, Izmir Katip Celebi University, Izmir, Turkey.
FAU - Karaman, Ozan
AU  - Karaman O
AUID- ORCID: 0000-0002-4175-4402
AD  - Tissue Engineering and Regenerative Medicine Laboratory, Department of Biomedical 
      Engineering, Izmir Katip Celebi University, Izmir, Turkey.
AD  - Bonegraft Biomaterials Co., Ege University Technopolis, Bornova, Izmir, Turkey.
LA  - eng
PT  - Journal Article
DEP - 20190422
PL  - England
TA  - Regen Biomater
JT  - Regenerative biomaterials
JID - 101652150
PMC - PMC6683955
OTO - NOTNLM
OT  - biomineralization
OT  - bone tissue engineering
OT  - nanofibers
OT  - non-thermal atmospheric plasma
EDAT- 2019/08/14 06:00
MHDA- 2019/08/14 06:01
PMCR- 2019/04/22
CRDT- 2019/08/13 06:00
PHST- 2018/12/27 00:00 [received]
PHST- 2019/02/14 00:00 [revised]
PHST- 2019/03/11 00:00 [accepted]
PHST- 2019/08/13 06:00 [entrez]
PHST- 2019/08/14 06:00 [pubmed]
PHST- 2019/08/14 06:01 [medline]
PHST- 2019/04/22 00:00 [pmc-release]
AID - rbz014 [pii]
AID - 10.1093/rb/rbz014 [doi]
PST - ppublish
SO  - Regen Biomater. 2019 Aug;6(4):231-240. doi: 10.1093/rb/rbz014. Epub 2019 Apr 22.