PMID- 31404881
OWN - NLM
STAT- MEDLINE
DCOM- 20200131
LR  - 20200131
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 142
DP  - 2019 Oct 1
TI  - Coupling photoelectrochemical and electrochemical strategies in one probe 
      electrode: Toward sensitive and reliable dual-signal bioassay for uracil-DNA 
      glycosylase activity.
PG  - 111569
LID - S0956-5663(19)30648-7 [pii]
LID - 10.1016/j.bios.2019.111569 [doi]
AB  - Uracil-DNA glycosylase (UDG) is a typical initiator for base excision repair 
      (BER) process. Since aberrant expression of UDG is relevant to a variety of 
      cancers, analysis of UDG activity with high sensitivity and accuracy is of great 
      importance. We reported herein a sensitive and reliable dual-signal bioassay for 
      UDG activity by coupling photoelectrochemical (PEC) and electrochemical (EC) 
      strategies in one probe electrode. The Au/TiO(2) hybrid was used as a matrix to 
      immobilize substrate DNA (sDNA), which modified with AgInS(2) quantum dot (AIS 
      QD) on the terminal. When UDG exist, the base of uracil was eliminated from the 
      sDNA, and the produced apyrimidinic (AP) site could be cleaved by endonuclease IV 
      (Endo. IV) immediately. Under this situation, the PEC labels of AIS QDs were 
      detached from the electrode, resulting in a "signal-off" trend for PEC signal. 
      After assistant DNA (aDNA) was then assembled, the hybridization chain reaction 
      (HCR) was triggered, and EC labels of ferrocene molecules were introduced, 
      producing a "signal-on" trend for EC signal. Besides, as the produced long 
      double-stranded DNA by the HCR had evident steric hindrance, the PEC signal 
      further decreased. Based on this meticulous design, the dual-signal bioassay for 
      UDG activity showed low detection limits of 4.3 x 10(-5) and 1.9 x 10(-4) U/mL 
      with PEC and EC detection, and accurate analysis of UDG activity in living cells 
      was realized. By just changing the recognition site, this sensitive and reliable 
      dual-signal strategy can be extended to diagnose other DNA repair-related enzymes 
      in the real samples.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Lu, Yanwei
AU  - Lu Y
AD  - Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life 
      Science, MOE, Shandong Key Laboratory of Biochemical Analysis, College of 
      Chemistry and Molecular Engineering, Qingdao University of Science and 
      Technology, Qingdao, 266042, China.
FAU - Zhao, Huan
AU  - Zhao H
AD  - Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life 
      Science, MOE, Shandong Key Laboratory of Biochemical Analysis, College of 
      Chemistry and Molecular Engineering, Qingdao University of Science and 
      Technology, Qingdao, 266042, China.
FAU - Fan, Gao-Chao
AU  - Fan GC
AD  - Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life 
      Science, MOE, Shandong Key Laboratory of Biochemical Analysis, College of 
      Chemistry and Molecular Engineering, Qingdao University of Science and 
      Technology, Qingdao, 266042, China. Electronic address: gcfan@qust.edu.cn.
FAU - Luo, Xiliang
AU  - Luo X
AD  - Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life 
      Science, MOE, Shandong Key Laboratory of Biochemical Analysis, College of 
      Chemistry and Molecular Engineering, Qingdao University of Science and 
      Technology, Qingdao, 266042, China. Electronic address: xiliangluo@qust.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20190802
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Immobilized Nucleic Acids)
RN  - 15FIX9V2JP (titanium dioxide)
RN  - 56HH86ZVCT (Uracil)
RN  - 7440-57-5 (Gold)
RN  - D1JT611TNE (Titanium)
RN  - EC 3.2.2.- (Uracil-DNA Glycosidase)
SB  - IM
MH  - Biosensing Techniques/*instrumentation
MH  - Cell Line, Tumor
MH  - Electrochemical Techniques/*instrumentation
MH  - Electrodes
MH  - Enzyme Assays/instrumentation
MH  - Equipment Design
MH  - Gold/chemistry
MH  - Humans
MH  - Immobilized Nucleic Acids/chemistry
MH  - Limit of Detection
MH  - Quantum Dots/chemistry
MH  - Titanium/chemistry
MH  - Uracil/chemistry
MH  - Uracil-DNA Glycosidase/*analysis
OTO - NOTNLM
OT  - Dual-signal bioassay
OT  - Electrochemistry
OT  - Photoelectrochemistry
OT  - Uracil-DNA glycosylase
EDAT- 2019/08/14 06:00
MHDA- 2020/02/01 06:00
CRDT- 2019/08/13 06:00
PHST- 2019/06/13 00:00 [received]
PHST- 2019/07/23 00:00 [revised]
PHST- 2019/08/02 00:00 [accepted]
PHST- 2019/08/14 06:00 [pubmed]
PHST- 2020/02/01 06:00 [medline]
PHST- 2019/08/13 06:00 [entrez]
AID - S0956-5663(19)30648-7 [pii]
AID - 10.1016/j.bios.2019.111569 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2019 Oct 1;142:111569. doi: 10.1016/j.bios.2019.111569. Epub 
      2019 Aug 2.