PMID- 31405193
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 2076-3921 (Print)
IS  - 2076-3921 (Electronic)
IS  - 2076-3921 (Linking)
VI  - 8
IP  - 8
DP  - 2019 Aug 10
TI  - Chemistry and Pharmacology of the Kazakh Crataegus Almaatensis Pojark: An Asian 
      Herbal Medicine.
LID - 10.3390/antiox8080300 [doi]
LID - 300
AB  - Crataegus almaatensis, an endemic ornamental plant in Kazakhstan is used in 
      popular medicine due to its cardiotonic properties. The most studied species of 
      the same genus are commonly found in Europe, which shows the importance of having 
      the Kazakh species validated via its chemical and pharmacological studies. 
      High-speed countercurrent chromatography (HSCCC) operated under optimized 
      conditions enabled an isolation of the three main compounds from the aqueous 
      phase of the leaves ethanol extract, further identified by nuclear magnetic 
      resonance (NMR), as quercetin 3-O-rhamnoside (quercitrin) (4.02% of the crude 
      extract-CECa); quercetin 3-O-beta-galactoside (hyperoside) (1.82% of CECa); 
      kaempferol 3-O-alpha-L-rhamnoside (afzelin) (0.94% of CECa). The CECa, the aqueous 
      phase of the crude extract (APCa) together with the isolates were evaluated for 
      their vascular (vascular reactivity in human internal mammary artery-HIMA), 
      anti-nociceptive (formalin-induced liking response and hot plate) and 
      anti-inflammatory (subcutaneous air-pouch model-SAP) activities. CECa at the 
      concentrations of 0.014 and 0.14 mg/mL significantly increased the maximum 
      contractility response of HIMA to noradrenaline. The APCa CR curve (0.007-0.7 
      mg/mL) showed an intrinsic relaxation effect of the HIMA. APCa at the dose of 100 
      mg/kg i.p. significantly decreased the total leukocyte count and the IL-1beta 
      release in the SAP wash.
FAU - Soares, Sabrina S
AU  - Soares SS
AUID- ORCID: 0000-0003-0088-0362
AD  - Laboratory of Pharmacy and Pharmaceutical care, Faculty of Pharmacy, University 
      of Coimbra, 3000-548 Coimbra, Portugal.
AD  - School of Pharmacy and Pharmaceutical Sciences & Trinity Biomedical Sciences 
      Institute, Trinity College Dublin, Dublin 2 D02 PN40, Ireland.
FAU - Bekbolatova, Elmira
AU  - Bekbolatova E
AD  - School of Pharmacy, JSC National Medical University, 050000 Almaty, Kazakhstan.
FAU - Cotrim, Maria Dulce
AU  - Cotrim MD
AD  - Laboratory of Pharmacy and Pharmaceutical care, Faculty of Pharmacy, University 
      of Coimbra, 3000-548 Coimbra, Portugal.
AD  - Coimbra Institute for Clinical and Biomedical Research (iCBR), Faculty of 
      Medicine, University of Coimbra, 3000-548 Coimbra, Portugal.
AD  - CIBB Center for Innovative Biomedicine and Biotechnology, University of Coimbra, 
      3000-548 Coimbra, Portugal.
FAU - Sakipova, Zuriyadda
AU  - Sakipova Z
AD  - School of Pharmacy, JSC National Medical University, 050000 Almaty, Kazakhstan.
FAU - Ibragimova, Liliya
AU  - Ibragimova L
AD  - School of Pharmacy, JSC National Medical University, 050000 Almaty, Kazakhstan.
FAU - Kukula-Koch, Wirginia
AU  - Kukula-Koch W
AUID- ORCID: 0000-0001-7076-600X
AD  - Department of Pharmacognosy with Medicinal Plants Unit, Medical University of 
      Lublin, 1 Chodzki str., 20-093 Lublin, Poland. virginia.kukula@gmail.com.
FAU - Giorno, Thais B Sardella
AU  - Giorno TBS
AD  - Laboratorio da Dor e Inflamacao, Universidade Federal do Rio de Janeiro, 
      21941-902 Rio de Janeiro, Brazil.
FAU - Fernandes, Patricia D
AU  - Fernandes PD
AD  - Laboratorio da Dor e Inflamacao, Universidade Federal do Rio de Janeiro, 
      21941-902 Rio de Janeiro, Brazil.
FAU - Fonseca, Diogo Andre
AU  - Fonseca DA
AUID- ORCID: 0000-0002-8244-139X
AD  - Laboratory of Pharmacy and Pharmaceutical care, Faculty of Pharmacy, University 
      of Coimbra, 3000-548 Coimbra, Portugal.
AD  - Coimbra Institute for Clinical and Biomedical Research (iCBR), Faculty of 
      Medicine, University of Coimbra, 3000-548 Coimbra, Portugal.
AD  - CIBB Center for Innovative Biomedicine and Biotechnology, University of Coimbra, 
      3000-548 Coimbra, Portugal.
FAU - Boylan, Fabio
AU  - Boylan F
AD  - School of Pharmacy and Pharmaceutical Sciences & Trinity Biomedical Sciences 
      Institute, Trinity College Dublin, Dublin 2 D02 PN40, Ireland. 
      fabio.boylan@tcd.ie.
LA  - eng
PT  - Journal Article
DEP - 20190810
PL  - Switzerland
TA  - Antioxidants (Basel)
JT  - Antioxidants (Basel, Switzerland)
JID - 101668981
PMC - PMC6720545
OTO - NOTNLM
OT  - Crataegus almaatensis Pojark
OT  - Flavonoids
OT  - HSCCC
OT  - Kazakhstan
OT  - anti-inflammatory activity
OT  - vascular activity
COIS- The authors declare no conflict of interest.
EDAT- 2019/08/14 06:00
MHDA- 2019/08/14 06:01
PMCR- 2019/08/10
CRDT- 2019/08/14 06:00
PHST- 2019/06/26 00:00 [received]
PHST- 2019/08/01 00:00 [revised]
PHST- 2019/08/06 00:00 [accepted]
PHST- 2019/08/14 06:00 [entrez]
PHST- 2019/08/14 06:00 [pubmed]
PHST- 2019/08/14 06:01 [medline]
PHST- 2019/08/10 00:00 [pmc-release]
AID - antiox8080300 [pii]
AID - antioxidants-08-00300 [pii]
AID - 10.3390/antiox8080300 [doi]
PST - epublish
SO  - Antioxidants (Basel). 2019 Aug 10;8(8):300. doi: 10.3390/antiox8080300.