PMID- 31405388
OWN - NLM
STAT- MEDLINE
DCOM- 20200708
LR  - 20200708
IS  - 1469-8161 (Electronic)
IS  - 0031-1820 (Linking)
VI  - 146
IP  - 14
DP  - 2019 Dec
TI  - Glutathione-S-transferase of Trichinella spiralis regulates maturation and 
      function of dendritic cells.
PG  - 1725-1732
LID - 10.1017/S003118201900115X [doi]
AB  - Immunomodulation by molecules from Trichinella spiralis (T. spiralis) has been 
      widely reported. Glutathione-S-transferase (GST) is a major immune-modulator of 
      the family of detoxification enzymes. Dendritic cells (DCs) are an important 
      target for the regulation of the immune response by T. spiralis. In this study, 
      the recombinant GST of T. spiralis (rTs-GST) was expressed and purified. rTs-GST 
      induced low CD40 expression and moderate CD80, CD86 and MHC-II expressions and 
      inhibited the increase of CD40, CD80 and CD86 on DCs induced by LPS. We showed 
      that rTs-GST decreased the LPS-induced elevated level of pro-inflammatory 
      cytokines of DCs and enhanced the level of regulatory cytokines IL-10 and TGF-beta. 
      Furthermore, co-culture of DCs and CD4+ T cells demonstrated that rTs-GST-treated 
      DCs suppressed the proliferation of OVA-specific CD4+ T cells and increased the 
      population of regulatory T cells (Tregs). rTs-GST-treated DCs induced a higher 
      level of IL-4, IL-10 and TGF-beta, but inhibited the level of IFN-gamma. This indicates 
      that rTs-GST-pulsed DCs induce both Th2-type responses and Tregs. These findings 
      contribute to the current understanding of the immunomodulation of Ts-GST on 
      cellular response and immunomodulation of T. spiralis.
FAU - Jin, Xuemin
AU  - Jin X
AD  - Key Laboratory of Zoonosis Research, Ministry of Education, Institute of 
      Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, 
      China.
FAU - Yang, Yong
AU  - Yang Y
AD  - Key Laboratory of Zoonosis Research, Ministry of Education, Institute of 
      Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, 
      China.
FAU - Liu, Xiaolei
AU  - Liu X
AD  - Key Laboratory of Zoonosis Research, Ministry of Education, Institute of 
      Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, 
      China.
FAU - Shi, Haining
AU  - Shi H
AD  - Mucosal Immunology Laboratory, Pediatric Gastroenterology Unit, Massachusetts 
      General Hospital, Boston, MA, USA.
FAU - Cai, Xuepeng
AU  - Cai X
AD  - China Institute of Veterinary Drugs Control, Beijing 100000, China.
AD  - State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of 
      Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, 
      CAAS, Lanzhou 730046, China.
FAU - Luo, Xuenong
AU  - Luo X
AD  - State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of 
      Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, 
      CAAS, Lanzhou 730046, China.
FAU - Liu, Mingyuan
AU  - Liu M
AD  - Key Laboratory of Zoonosis Research, Ministry of Education, Institute of 
      Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, 
      China.
AD  - Jiangsu Co-innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou 225000, China.
FAU - Bai, Xue
AU  - Bai X
AUID- ORCID: 0000-0001-5725-2442
AD  - Key Laboratory of Zoonosis Research, Ministry of Education, Institute of 
      Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190830
PL  - England
TA  - Parasitology
JT  - Parasitology
JID - 0401121
RN  - 0 (Cytokines)
RN  - 0 (IL10 protein, mouse)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 130068-27-8 (Interleukin-10)
RN  - 207137-56-2 (Interleukin-4)
RN  - EC 2.5.1.18 (Glutathione Transferase)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/immunology
MH  - Cytokines/immunology
MH  - Dendritic Cells/*immunology/*parasitology
MH  - Female
MH  - Glutathione/metabolism
MH  - Glutathione Transferase/*immunology
MH  - Interleukin-10/immunology
MH  - Interleukin-4/immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Recombinant Proteins/immunology
MH  - T-Lymphocytes/immunology
MH  - Transforming Growth Factor beta1/immunology
MH  - Trichinella spiralis/*enzymology/immunology
OTO - NOTNLM
OT  - Dendritic cells
OT  - Th2 immune response
OT  - Tregs
OT  - Trichinella spiralis
OT  - glutathione-S-transferase
OT  - semi-maturation
EDAT- 2019/08/14 06:00
MHDA- 2020/07/09 06:00
CRDT- 2019/08/14 06:00
PHST- 2019/08/14 06:00 [pubmed]
PHST- 2020/07/09 06:00 [medline]
PHST- 2019/08/14 06:00 [entrez]
AID - S003118201900115X [pii]
AID - 10.1017/S003118201900115X [doi]
PST - ppublish
SO  - Parasitology. 2019 Dec;146(14):1725-1732. doi: 10.1017/S003118201900115X. Epub 
      2019 Aug 30.