PMID- 3140603
OWN - NLM
STAT- MEDLINE
DCOM- 19881115
LR  - 20131121
IS  - 0884-0431 (Print)
IS  - 0884-0431 (Linking)
VI  - 1
IP  - 2
DP  - 1986 Apr
TI  - Polyamines are involved in the 1-alpha,25-dihydroxyvitamin D3-induced fusion of 
      mouse alveolar macrophages.
PG  - 235-42
AB  - We have reported that 1-alpha,25-dihydroxyvitamin D3 [1-alpha,25-(OH)2D3] 
      directly induces fusion of mouse alveolar macrophages at a very high rate (circa 
      70-80%) by a mechanism involving protein synthesis (Proc. Natl. Acad. Sci. USA 
      80:5583, 1983; FEBS Letters 174:61, 1984). While examining further the mechanism 
      of the 1-alpha,25-(OH)2D3-induced fusion of macrophages, we found that polyamines 
      are involved in this mechanism. Mouse alveolar macrophages incubated with 12 nM 
      1-alpha,25-(OH)2D3 began to fuse at 36 h and the fusion rate increased linearly 
      up to 60 h. Addition of as much as 0.05-5 mM alpha-difluoromethylornithine 
      (alpha-DFMO), a specific inhibitor of ornithine decarboxylase, did not inhibit 
      fusion appreciably, but addition of 0.05-5 microM methylglyoxal 
      bis(guanylhydrazone) (MGBG), an inhibitor of S-adenosylmethionine decarboxylase, 
      strikingly inhibited fusion. When macrophages were treated with both 12 nM 
      1-alpha,25-(OH)2D3 and 5 microM MGBG for the first 12 h and incubated further for 
      60 h in fresh medium containing 1-alpha,25-(OH)2D3, fusion was significantly 
      inhibited, suggesting that the 1-alpha,25-(OH)2D3-induced synthesis of polyamines 
      precedes fusion. The inhibition by MGBG of the 1-alpha,25-(OH)2D3-induced fusion 
      was restored completely by adding 1 microM spermidine or spermine or 100 microM 
      putrescine. None of the polyamines alone induced fusion. MGBG suppressed the 
      1-alpha,25-(OH)2D3-induced incorporation of [3H]-leucine into the trichloroacetic 
      acid-insoluble fraction in macrophages, but its inhibitory effect was restored 
      completely by adding 1 microM spermidine.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Hayashi, T
AU  - Hayashi T
AD  - Department of Biochemistry, School of Dentistry, Showa University, Tokyo, Japan.
FAU - Shinki, T
AU  - Shinki T
FAU - Tanaka, H
AU  - Tanaka H
FAU - Abe, E
AU  - Abe E
FAU - Suda, T
AU  - Suda T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Bone Miner Res
JT  - Journal of bone and mineral research : the official journal of the American 
      Society for Bone and Mineral Research
JID - 8610640
RN  - 0 (Polyamines)
RN  - 2FZ7Y3VOQX (Spermine)
RN  - FXC9231JVH (Calcitriol)
RN  - OD5Q0L447W (Mitoguazone)
RN  - U87FK77H25 (Spermidine)
RN  - ZQN1G5V6SR (Eflornithine)
SB  - IM
MH  - Animals
MH  - Calcitriol/*pharmacology
MH  - Cell Fusion/drug effects
MH  - Cells, Cultured
MH  - Eflornithine/pharmacology
MH  - Macrophages/*drug effects
MH  - Male
MH  - Mice
MH  - Mitoguazone/pharmacology
MH  - Polyamines/*pharmacology
MH  - Protein Biosynthesis
MH  - Pulmonary Alveoli/cytology/*drug effects
MH  - Spermidine/pharmacology
MH  - Spermine/pharmacology
EDAT- 1986/04/01 00:00
MHDA- 1986/04/01 00:01
CRDT- 1986/04/01 00:00
PHST- 1986/04/01 00:00 [pubmed]
PHST- 1986/04/01 00:01 [medline]
PHST- 1986/04/01 00:00 [entrez]
AID - 10.1002/jbmr.5650010211 [doi]
PST - ppublish
SO  - J Bone Miner Res. 1986 Apr;1(2):235-42. doi: 10.1002/jbmr.5650010211.