PMID- 31407284
OWN - NLM
STAT- MEDLINE
DCOM- 20200408
LR  - 20200603
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 2038
DP  - 2019
TI  - Programmable Chromosome Painting with Oligopaints.
PG  - 167-180
LID - 10.1007/978-1-4939-9674-2_11 [doi]
AB  - Current methods for chromosome painting via fluorescence in situ hybridization 
      (FISH) are costly, time-consuming, and limited in complexity. In contrast to 
      conventional sources of probe, Oligopaints are computationally designed, 
      synthesized on microarrays, and amplified by PCR. This approach allows for 
      precise control over the sequences they target, which can range from a few 
      kilobases to entire chromosomes with the same basic protocol. We have utilized 
      the flexibility and scalability of Oligopaints to generate low-cost and renewable 
      chromosome paints for Drosophila, mouse, and human chromosomes. These Oligopaint 
      libraries can be customized to label any genomic feature(s) in a chromosome-wide 
      manner. Additionally, this method is compatible with sequential FISH to label 
      entire genomes with a single denaturation step. Here, we outline a protocol and 
      considerations to scale the Oligopaint technology for fluorescent labeling of 
      whole chromosomes.
FAU - Nguyen, Son C
AU  - Nguyen SC
AD  - Department of Genetics, Penn Epigenetics Institute, Perelman School of Medicine, 
      University of Pennsylvania, Philadelphia, PA, USA.
FAU - Joyce, Eric F
AU  - Joyce EF
AD  - Department of Genetics, Penn Epigenetics Institute, Perelman School of Medicine, 
      University of Pennsylvania, Philadelphia, PA, USA. erjoyce@upenn.edu.
LA  - eng
GR  - R35 GM128903/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Oligonucleotides)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Chromosome Painting/*methods
MH  - Chromosomes, Human
MH  - *Chromosomes, Insect
MH  - *Chromosomes, Mammalian
MH  - Drosophila melanogaster/*genetics
MH  - Fluorescent Dyes/*metabolism
MH  - HCT116 Cells
MH  - Humans
MH  - Mice
MH  - *Microscopy, Fluorescence
MH  - Oligonucleotides/genetics/*metabolism
PMC - PMC7265757
MID - NIHMS1593562
OTO - NOTNLM
OT  - Chromosome painting
OT  - FISH
OT  - Oligonucleotide
OT  - Oligopaint
EDAT- 2019/08/14 06:00
MHDA- 2020/04/09 06:00
PMCR- 2020/06/02
CRDT- 2019/08/14 06:00
PHST- 2019/08/14 06:00 [entrez]
PHST- 2019/08/14 06:00 [pubmed]
PHST- 2020/04/09 06:00 [medline]
PHST- 2020/06/02 00:00 [pmc-release]
AID - 10.1007/978-1-4939-9674-2_11 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2019;2038:167-180. doi: 10.1007/978-1-4939-9674-2_11.