PMID- 31407591
OWN - NLM
STAT- MEDLINE
DCOM- 20200108
LR  - 20200108
IS  - 2169-141X (Electronic)
IS  - 2169-1401 (Linking)
VI  - 47
IP  - 1
DP  - 2019 Dec
TI  - Circular RNA ZFR accelerates non-small cell lung cancer progression by acting as 
      a miR-101-3p sponge to enhance CUL4B expression.
PG  - 3410-3416
LID - 10.1080/21691401.2019.1652623 [doi]
AB  - BACKGROUND: Non-small-cell lung carcinoma (NSCLC) is the most common type of lung 
      cancer. Circular RNA ZFR (circZFR) is an identified circular RNA (circRNA) that 
      is correlated with cancer progression. However, the role of circZFR in NSCLC 
      remains unknown. In the present study, we aimed to investigate the function of 
      circZFR in NSCLC and the underlying mechanism. METHODS: The expression patterns 
      of circZFR were determined using qRT-PCR in NSCLC samples and cell lines. The 
      subcellular distribution of circZFR in NSCLC cells was analyzed by fluorescence 
      in situ hybridization (FISH). Cell proliferation was examined utilizing the CCK-8 
      assay. Cell migration and invasion were evaluated using the Transwell assay. We 
      used the bioinformatics software TargetScan and miRanda to predict circRNA-miRNA 
      and miRNA-mRNA interactions. RESULTS: Our results showed that circZFR expressions 
      were significantly upregulated in NSCLC tissues and cell lines. Knockdown of 
      circZFR significantly inhibited the cell proliferation, migration and invasion of 
      NSCLC cells in vitro. Furthermore, we demonstrated that circZFR acted as a sponge 
      to absorb microRNA-101-3p (miR-101-3p) and promoted cullin 4B (CUL4B) expression. 
      CONCLUSIONS: Collectively, our results demonstrated that circZFR exhibited a 
      carcinogenic role by sponging miR-101-3p and regulating CUL4B expression in 
      NSCLC. These findings provided evidence for understanding the role of circZFR in 
      NSCLC tumourigenesis.
FAU - Zhang, Haifeng
AU  - Zhang H
AD  - a Department of Thoracic Surgery, Huaihe Hospital of Henan University , Kaifeng , 
      Henan , P. R. China.
FAU - Wang, Xiaolong
AU  - Wang X
AD  - a Department of Thoracic Surgery, Huaihe Hospital of Henan University , Kaifeng , 
      Henan , P. R. China.
FAU - Hu, Baoli
AU  - Hu B
AD  - a Department of Thoracic Surgery, Huaihe Hospital of Henan University , Kaifeng , 
      Henan , P. R. China.
FAU - Zhang, Feng
AU  - Zhang F
AD  - a Department of Thoracic Surgery, Huaihe Hospital of Henan University , Kaifeng , 
      Henan , P. R. China.
FAU - Wei, Haitao
AU  - Wei H
AD  - a Department of Thoracic Surgery, Huaihe Hospital of Henan University , Kaifeng , 
      Henan , P. R. China.
FAU - Li, Li
AU  - Li L
AD  - b College of Nursing and Health, Henan University , Kaifeng , Henan , P. R. 
      China.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Artif Cells Nanomed Biotechnol
JT  - Artificial cells, nanomedicine, and biotechnology
JID - 101594777
RN  - 0 (CUL4B protein, human)
RN  - 0 (Cullin Proteins)
RN  - 0 (MIRN101 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Circular)
SB  - IM
MH  - Base Sequence
MH  - Carcinogenesis/genetics
MH  - Carcinoma, Non-Small-Cell Lung/*pathology
MH  - Cell Line, Tumor
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - Cullin Proteins/*genetics
MH  - *Disease Progression
MH  - Gene Expression Regulation, Neoplastic/*genetics
MH  - Gene Knockdown Techniques
MH  - Humans
MH  - Lung Neoplasms/*pathology
MH  - MicroRNAs/*genetics
MH  - Neoplasm Invasiveness
MH  - RNA, Circular/*genetics
OTO - NOTNLM
OT  - Non-small-cell lung carcinoma (NSCLC)
OT  - cell proliferation
OT  - circular RNA ZFR (circZFR)
OT  - cullin 4B (CUL4B)
OT  - invasion
OT  - miR-101-3p
EDAT- 2019/08/14 06:00
MHDA- 2020/01/09 06:00
CRDT- 2019/08/14 06:00
PHST- 2019/08/14 06:00 [entrez]
PHST- 2019/08/14 06:00 [pubmed]
PHST- 2020/01/09 06:00 [medline]
AID - 10.1080/21691401.2019.1652623 [doi]
PST - ppublish
SO  - Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):3410-3416. doi: 
      10.1080/21691401.2019.1652623.