PMID- 31408766
OWN - NLM
STAT- MEDLINE
DCOM- 20200413
LR  - 20200413
IS  - 1567-7257 (Electronic)
IS  - 1567-1348 (Linking)
VI  - 74
DP  - 2019 Oct
TI  - Positioning Quebec ORF5 sequences of porcine reproductive and respiratory 
      syndrome virus (PRRSV) within Canada and worldwide diversity.
PG  - 103999
LID - S1567-1348(19)30227-8 [pii]
LID - 10.1016/j.meegid.2019.103999 [doi]
AB  - Sequencing of ORF5 gene is widely used and considered essential for diagnostics 
      and control of porcine reproductive and respiratory syndrome (PRRS) in Canada. 
      The objective of this study was to position Quebec ORF5 sequences of PRRS virus 
      within Canada and worldwide diversity. Overall, 76.8% of the 5204 sequences 
      gathered from Quebec (n = 5031), Ontario (n = 151) and Manitoba (n = 18) were 
      classified into one of 34 genetic clusters defined as groupings including >/=15 
      sequences and having >/=70% rapid bootstrap support value from a maximum likelihood 
      (ML)-phylogeny. Following the addition of PRRSV 2 international reference dataset 
      from Shi et al. (2010), the most predominant lineages in our dataset were 
      wild-type 1 and vaccine-like 5.1 (MLV) and 8.9 (ATP). No strains or only a very 
      few (1 or 2) were assigned to lineages 1.3-1.5, 3, 4, 5.2, 6, 7 or 9. Most 
      wild-type clusters (97%) detected in a dataset from Canada did not include any 
      sequence from the international reference dataset. It might reflect recent 
      subpopulations that were absent at the time of Shi's publication. As an example, 
      cluster #25 first appeared in 2007, but since then had expanded considerably and 
      is now the most prevalent wild-type cluster found in Quebec. A total of 117 RFLP 
      patterns were identified and those were poorly correlated with genetic clusters 
      based on phylogeny. Factors modulating PRRSV diversity such as pig movement that 
      occurred within and between provinces should be further investigated in a 
      perspective of disease control.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Lambert, Marie-Eve
AU  - Lambert ME
AD  - Laboratoire d'epidemiologie et de medecine porcine (LEMP), Faculty of Veterinary 
      Medicine, Universite de Montreal, St. Hyacinthe, Quebec, Canada; Swine and 
      Poultry Infectious Diseases Research Center (CRIPA), Faculty of Veterinary 
      Medicine, Universite de Montreal, St. Hyacinthe, Quebec, Canada. Electronic 
      address: marie-eve.lambert@umontreal.ca.
FAU - Delisle, Benjamin
AU  - Delisle B
AD  - Laboratoire d'epidemiologie et de medecine porcine (LEMP), Faculty of Veterinary 
      Medicine, Universite de Montreal, St. Hyacinthe, Quebec, Canada; Swine and 
      Poultry Infectious Diseases Research Center (CRIPA), Faculty of Veterinary 
      Medicine, Universite de Montreal, St. Hyacinthe, Quebec, Canada.
FAU - Arsenault, Julie
AU  - Arsenault J
AD  - Laboratoire d'epidemiologie et de medecine porcine (LEMP), Faculty of Veterinary 
      Medicine, Universite de Montreal, St. Hyacinthe, Quebec, Canada; Swine and 
      Poultry Infectious Diseases Research Center (CRIPA), Faculty of Veterinary 
      Medicine, Universite de Montreal, St. Hyacinthe, Quebec, Canada.
FAU - Poljak, Zvonimir
AU  - Poljak Z
AD  - Department of Population Medicine, Ontario Veterinary College, University of 
      Guelph, Ontario, Canada.
FAU - D'Allaire, Sylvie
AU  - D'Allaire S
AD  - Laboratoire d'epidemiologie et de medecine porcine (LEMP), Faculty of Veterinary 
      Medicine, Universite de Montreal, St. Hyacinthe, Quebec, Canada; Swine and 
      Poultry Infectious Diseases Research Center (CRIPA), Faculty of Veterinary 
      Medicine, Universite de Montreal, St. Hyacinthe, Quebec, Canada.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190810
PL  - Netherlands
TA  - Infect Genet Evol
JT  - Infection, genetics and evolution : journal of molecular epidemiology and 
      evolutionary genetics in infectious diseases
JID - 101084138
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Animals
MH  - *Genetic Variation
MH  - Likelihood Functions
MH  - Manitoba
MH  - Ontario
MH  - Phylogeny
MH  - Polymorphism, Restriction Fragment Length
MH  - Porcine respiratory and reproductive syndrome virus/*classification/genetics
MH  - Quebec
MH  - Swine
MH  - Viral Envelope Proteins/*genetics
OTO - NOTNLM
OT  - Classification
OT  - Genetic diversity
OT  - ORF5
OT  - PRRS
OT  - Phylogeny
OT  - RFLP
OT  - Sequence
EDAT- 2019/08/14 06:00
MHDA- 2020/04/14 06:00
CRDT- 2019/08/14 06:00
PHST- 2019/03/21 00:00 [received]
PHST- 2019/08/06 00:00 [revised]
PHST- 2019/08/08 00:00 [accepted]
PHST- 2019/08/14 06:00 [pubmed]
PHST- 2020/04/14 06:00 [medline]
PHST- 2019/08/14 06:00 [entrez]
AID - S1567-1348(19)30227-8 [pii]
AID - 10.1016/j.meegid.2019.103999 [doi]
PST - ppublish
SO  - Infect Genet Evol. 2019 Oct;74:103999. doi: 10.1016/j.meegid.2019.103999. Epub 
      2019 Aug 10.