PMID- 31408874
OWN - NLM
STAT- MEDLINE
DCOM- 20200210
LR  - 20200210
IS  - 1423-0259 (Electronic)
IS  - 0030-3747 (Linking)
VI  - 63
IP  - 1
DP  - 2020
TI  - Ultraviolet Radiation Exposure of One Eye Stimulates Sympathizing Expression of 
      Neurokinin-1 Receptor but Not Monocyte Chemoattractant Protein-1 in the Partner 
      Eye.
PG  - 59-71
LID - 10.1159/000501320 [doi]
AB  - PURPOSE: To investigate the influence of unilateral ultraviolet radiation (UVR) 
      exposure on the unexposed, partner eye in vivo. To characterize the immunological 
      cross-talk between the eyes and verify a sympathizing reaction of the partner eye 
      via a neurokinin-dependent signaling pathway of substance P and its neurokinin-1 
      receptor (NKR-1) and/or monocyte chemoattractant protein-1 (MCP-1). METHODS: 
      C57BL/6 mice were unilaterally exposed in vivo to UVR-B to a 5-fold cataract 
      threshold equivalent dose of 14.5 kJ/m2 with a UV irradiation Bio-Spectra system. 
      The unexposed contralateral eye was completely shielded during irradiation. After 
      3 and 7 days post exposure, eyes were stained with fluorescence-coupled antibody 
      for substance P NKR-1. The same was performed in control animals receiving only 
      anesthesia but no UVR-B exposure. NKR-1 and MCP-1 levels in ocular tissue lysates 
      were quantified by enzyme-linked immunosorbent assay. RESULTS: UVR-B induces 
      NKR-1 upregulation after 3 and 7 days in the exposed and in the unexposed, 
      contralateral mouse eye. NKR-1 protein level was upregulated in the exposed and 
      contralateral iris/ciliary body complex, choroidea and in the contralateral 
      retina as well as in the exposed cornea. MCP-1 levels were elevated in the 
      exposed cornea, iris/ciliary body complex, and aqueous humor but not in 
      contralateral ocular tissues. CONCLUSIONS: UVR-B exposure triggers NKR-1 
      upregulation not only in the exposed but also in the unexposed, partner eye in 
      various ocular tissues. Following UVR-B exposure, MCP-1 protein levels are 
      upregulated in the exposed eye, but the contralateral side remains unaffected.
CI  - (c) 2019 S. Karger AG, Basel.
FAU - Gross, Janine
AU  - Gross J
AD  - Department of Ophthalmology, University of Bonn, Bonn, Germany, 
      Janine.Gross@ukbonn.de.
FAU - Willimsky, Eva
AU  - Willimsky E
AD  - Department of Ophthalmology, University of Bonn, Bonn, Germany.
FAU - Wegener, Alfred R
AU  - Wegener AR
AD  - Department of Ophthalmology, University of Bonn, Bonn, Germany.
FAU - Kronschlager, Martin
AU  - Kronschlager M
AD  - Department of Ophthalmology, Hanusch Hospital, Vienna, Austria.
FAU - Schonfeld, Carl-Ludwig
AU  - Schonfeld CL
AD  - Herzog Carl Theodor Eye Clinic, Munich, Germany.
AD  - Department of Ophthalomology, Ludwig-Maximilians University, Munich, Germany.
FAU - Holz, Frank G
AU  - Holz FG
AD  - Department of Ophthalmology, University of Bonn, Bonn, Germany.
FAU - Meyer, Linda M
AU  - Meyer LM
AD  - Department of Ophthalmology, University of Bonn, Bonn, Germany.
AD  - Herzog Carl Theodor Eye Clinic, Munich, Germany.
LA  - eng
PT  - Journal Article
DEP - 20190813
PL  - Switzerland
TA  - Ophthalmic Res
JT  - Ophthalmic research
JID - 0267442
RN  - 0 (Chemokine CCL2)
RN  - 0 (Receptors, Neurokinin-1)
SB  - IM
MH  - Animals
MH  - Chemokine CCL2/*metabolism
MH  - *Eye/metabolism/radiation effects
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Receptors, Neurokinin-1/*metabolism
MH  - Ultraviolet Rays/*adverse effects
MH  - Up-Regulation
OTO - NOTNLM
OT  - Cataract
OT  - Inflammation
OT  - Monocyte chemoattractant protein-1
OT  - Neurokinin-1 receptor
OT  - Ultraviolet radiation B
EDAT- 2019/08/14 06:00
MHDA- 2020/02/11 06:00
CRDT- 2019/08/14 06:00
PHST- 2019/01/29 00:00 [received]
PHST- 2019/05/28 00:00 [accepted]
PHST- 2019/08/14 06:00 [pubmed]
PHST- 2020/02/11 06:00 [medline]
PHST- 2019/08/14 06:00 [entrez]
AID - 000501320 [pii]
AID - 10.1159/000501320 [doi]
PST - ppublish
SO  - Ophthalmic Res. 2020;63(1):59-71. doi: 10.1159/000501320. Epub 2019 Aug 13.