PMID- 31410159
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 18
IP  - 3
DP  - 2019 Sep
TI  - Protective role of thymoquinone in sepsis-induced liver injury in BALB/c mice.
PG  - 1985-1992
LID - 10.3892/etm.2019.7779 [doi]
AB  - Sepsis increases the risk of developing liver injury. Previous studies have 
      demonstrated that thymoquinone (TQ) exhibits hepatoprotective properties in vivo 
      as well as in vitro. The present study aimed to investigate the underlying 
      mechanisms of the protective effects of TQ against liver injury in septic BALB/c 
      mice. Male BALB/c mice (age, 8 weeks) were randomly divided into four groups, 
      namely, the control, TQ (50 mg/kg/day) treatment, cecal ligation and puncture 
      (CLP), and TQ + CLP groups. CLP was performed following gavage of TQ for 2 weeks. 
      At 48 h post-CLP, the histopathological alterations in the liver tissue (LT) and 
      plasma levels of serum alanine aminotransferase (ALT), aspartate aminotransferase 
      (AST) and alkaline phosphatase (ALP) were assessed. The present study evaluated 
      microtubule-associated protein light chain 3 (LC3), sequestosome-1 (p62) and 
      beclin 1 protein expression by western blotting and immunostaining, as well as 
      interleukin (IL)-6, IL-1beta, IL-10, monocyte chemoattractant protein-1 (MCP-1) and 
      tumor necrosis factor-alpha (TNF-alpha) mRNA expression by RT-qPCR. The results of the 
      present study indicated that administration of TQ to mice reduced the 
      histological alterations caused by CLP in LT. TQ inhibited the plasma levels of 
      ALT, AST and ALP in the CLP group. TQ significantly inhibited the elevation of 
      p62, IL-1beta, IL-6, MCP-1 and TNF-alpha levels as well as increased the LC3, beclin 1 
      and IL-10 levels in LT. PI3K expression in the TQ + CLP group was significantly 
      decreased compared with that in the CLP group. TQ treatment effectively modulated 
      the expression levels of p62, LC3, beclin 1, PI3K and proinflammatory cytokines, 
      and may be an important agent for the treatment of sepsis-induced liver injury.
FAU - Wang, Fei
AU  - Wang F
AD  - Department of Gastroenterology, Affiliated Zhongshan Hospital of Dalian 
      University, Dalian, Liaoning 116001, P.R. China.
FAU - Lei, Xiong
AU  - Lei X
AD  - Graduate School of Dalian Medical University, The First Clinical College, Dalian, 
      Liaoning 116044, P.R. China.
FAU - Zhao, Yue
AU  - Zhao Y
AD  - Graduate School of Dalian Medical University, The First Clinical College, Dalian, 
      Liaoning 116044, P.R. China.
FAU - Yu, Qinggong
AU  - Yu Q
AD  - Department of Gastroenterology, Affiliated Zhongshan Hospital of Dalian 
      University, Dalian, Liaoning 116001, P.R. China.
FAU - Li, Qianwei
AU  - Li Q
AD  - Department of Gastroenterology, Affiliated Zhongshan Hospital of Dalian 
      University, Dalian, Liaoning 116001, P.R. China.
FAU - Zhao, Hui
AU  - Zhao H
AD  - Department of Vascular Surgery, Affiliated Zhongshan Hospital of Dalian 
      University, Dalian, Liaoning 116001, P.R. China.
FAU - Pei, Zuowei
AU  - Pei Z
AD  - Department of Cardiology, Affiliated Zhongshan Hospital of Dalian University, 
      Dalian, Liaoning 116001, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20190717
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC6676142
OTO - NOTNLM
OT  - BALB/c mice
OT  - autophagy
OT  - liver injury
OT  - sepsis
OT  - thymoquinone
EDAT- 2019/08/15 06:00
MHDA- 2019/08/15 06:01
PMCR- 2019/07/17
CRDT- 2019/08/15 06:00
PHST- 2018/09/16 00:00 [received]
PHST- 2019/06/06 00:00 [accepted]
PHST- 2019/08/15 06:00 [entrez]
PHST- 2019/08/15 06:00 [pubmed]
PHST- 2019/08/15 06:01 [medline]
PHST- 2019/07/17 00:00 [pmc-release]
AID - ETM-0-0-7779 [pii]
AID - 10.3892/etm.2019.7779 [doi]
PST - ppublish
SO  - Exp Ther Med. 2019 Sep;18(3):1985-1992. doi: 10.3892/etm.2019.7779. Epub 2019 Jul 
      17.