PMID- 31412950
OWN - NLM
STAT- MEDLINE
DCOM- 20200713
LR  - 20200713
IS  - 1757-6512 (Electronic)
IS  - 1757-6512 (Linking)
VI  - 10
IP  - 1
DP  - 2019 Aug 14
TI  - Design of experiments to assess the effect of culture parameters on the 
      osteogenic differentiation of human adipose stromal cells.
PG  - 256
LID - 10.1186/s13287-019-1333-7 [doi]
LID - 256
AB  - BACKGROUND: Human adipose-derived stromal cells (hASCs) have been gaining 
      increasing popularity in regenerative medicine thanks to their multipotency, ease 
      of collection, and efficient culture. Similarly to other stromal cells, their 
      function is particularly sensitive to the culture conditions, including the 
      composition of the culture medium. Given the large number of parameters that can 
      play a role in their specification, the rapid assessment would be beneficial to 
      allow the optimization of their culture parameters. METHOD: Herein we used the 
      design of experiments (DOE) method to rapidly screen the influence and relevance 
      of several culture parameters on the osteogenic differentiation of hASCs. 
      Specifically, seven cell culture parameters were selected for this study based on 
      a literature review. These parameters included the source of hASCs (the different 
      providers having different methods for processing the cells prior to their 
      external use), the source of serum (fetal bovine serum vs. human platelet 
      lysate), and several soluble osteoinductive factors, including dexamethasone and 
      a potent growth factor, the bone morphogenetic protein-9 (BMP-9). The expression 
      of alkaline phosphatase was quantified as a readout for the osteogenic 
      differentiation of hASCs. RESULTS: The DOE analysis enabled to classify the seven 
      studied parameters according to their relative influence on the osteogenic 
      differentiation of hASCs. Notably, the source of serum was found to have a major 
      effect on the osteogenic differentiation of hASCs as well as their origin 
      (different providers) and the presence of L-ascorbate-2-phosphate and BMP-9. 
      CONCLUSION: The DOE-based screening is a valuable approach for the classification 
      of the impact of several cell culture parameters on the osteogenic 
      differentiation of hASCs.
FAU - Kuterbekov, Mirasbek
AU  - Kuterbekov M
AD  - Universite catholique de Louvain, Institute of Condensed Matter & Nanosciences 
      (Bio & Soft Matter), Croix du Sud 1, Box L7.04.02, 1348, Louvain-la-Neuve, 
      Belgium.
AD  - CNRS, LMGP, 3 parvis Louis Neel, 38016, Grenoble, France.
AD  - Grenoble Institute of Technology, University Grenoble Alpes, LMGP, 3 parvis Louis 
      Neel, 38016, Grenoble, France.
FAU - Machillot, Paul
AU  - Machillot P
AD  - CNRS, LMGP, 3 parvis Louis Neel, 38016, Grenoble, France.
AD  - Grenoble Institute of Technology, University Grenoble Alpes, LMGP, 3 parvis Louis 
      Neel, 38016, Grenoble, France.
FAU - Baillet, Francis
AU  - Baillet F
AD  - Universite Grenoble Alpes, CNRS, Grenoble INP, SIMAP, 1130 rue de la Piscine, 
      38402, Saint-Martin d'Heres, France.
FAU - Jonas, Alain M
AU  - Jonas AM
AD  - Universite catholique de Louvain, Institute of Condensed Matter & Nanosciences 
      (Bio & Soft Matter), Croix du Sud 1, Box L7.04.02, 1348, Louvain-la-Neuve, 
      Belgium.
FAU - Glinel, Karine
AU  - Glinel K
AD  - Universite catholique de Louvain, Institute of Condensed Matter & Nanosciences 
      (Bio & Soft Matter), Croix du Sud 1, Box L7.04.02, 1348, Louvain-la-Neuve, 
      Belgium.
FAU - Picart, Catherine
AU  - Picart C
AUID- ORCID: 0000-0003-0130-1000
AD  - CNRS, LMGP, 3 parvis Louis Neel, 38016, Grenoble, France. 
      catherine.picart@grenoble-inp.fr.
AD  - Grenoble Institute of Technology, University Grenoble Alpes, LMGP, 3 parvis Louis 
      Neel, 38016, Grenoble, France. catherine.picart@grenoble-inp.fr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190814
PL  - England
TA  - Stem Cell Res Ther
JT  - Stem cell research & therapy
JID - 101527581
SB  - IM
MH  - Cell Culture Techniques/*methods
MH  - *Cell Differentiation
MH  - *Cell Proliferation
MH  - Cells, Cultured
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology/metabolism
MH  - Osteoblasts/*cytology/metabolism
MH  - *Osteogenesis
MH  - *Research Design
MH  - Tissue Engineering
PMC - PMC6694725
OTO - NOTNLM
OT  - Cell therapy
OT  - Design of experiments
OT  - Human adipose stromal cell
OT  - Osteogenic differentiation
COIS- The authors declare that they have no competing interests.
EDAT- 2019/08/16 06:00
MHDA- 2020/07/14 06:00
PMCR- 2019/08/14
CRDT- 2019/08/16 06:00
PHST- 2019/06/28 00:00 [received]
PHST- 2019/07/08 00:00 [accepted]
PHST- 2019/06/28 00:00 [revised]
PHST- 2019/08/16 06:00 [entrez]
PHST- 2019/08/16 06:00 [pubmed]
PHST- 2020/07/14 06:00 [medline]
PHST- 2019/08/14 00:00 [pmc-release]
AID - 10.1186/s13287-019-1333-7 [pii]
AID - 1333 [pii]
AID - 10.1186/s13287-019-1333-7 [doi]
PST - epublish
SO  - Stem Cell Res Ther. 2019 Aug 14;10(1):256. doi: 10.1186/s13287-019-1333-7.