PMID- 31415968
OWN - NLM
STAT- MEDLINE
DCOM- 20191119
LR  - 20191119
IS  - 1879-2448 (Electronic)
IS  - 0043-1354 (Linking)
VI  - 164
DP  - 2019 Nov 1
TI  - Adhesion of filamentous fungi isolated from drinking water under different 
      process conditions.
PG  - 114951
LID - S0043-1354(19)30725-0 [pii]
LID - 10.1016/j.watres.2019.114951 [doi]
AB  - Filamentous fungi (ff) are consistently recognized as drinking water (DW) 
      inhabitants, typically harboured in biofilms. Their sessile behaviour is still 
      poorly understood. This study aimed the evaluation of the influence of several 
      abiotic factors (substratum, hydrodynamic conditions and nutrient availability) 
      on biofilm formation by Penicillium brevicompactum and P. expansum isolated from 
      DW. Fungal adhesion was quantified on high density polyethylene (HDPE) and 
      polyvinyl chloride (PVC) surfaces using synthetic tap water (STW) and R2A broth, 
      under stagnant or agitated (25 and 150 rpm) conditions. Fungal spore numbers were 
      assessed after staining with Calcofluor White MR2 and epifluorescence microscopy. 
      The surface charge and physicochemical properties of spores were characterized 
      for a mechanistic understanding on the adhesion process. The adhesion kinetics of 
      spores was represented accurately by the Logistic model, in which adhesion 
      increased with time until a maximum level attained before spore germination (8 h 
      after incubation). In general, P. brevicompactum demonstrated to adhere in a 
      higher extent than P. expansum (12 x 10(4) spores/cm(2)vs 1.7 x 10(4) 
      spores/cm(2)). Moreover, fungal adhesion was potentiated under stagnation and 
      using R2A broth. HDPE and PVC allowed spore adhesion at similar extents. Adhesion 
      predictions based on the extended Derjaguin, Landau, Verwey and Overbeek (XDLVO) 
      theory corroborated the experimental results, highlighting the role of 
      physicochemical surface properties on the adhesion of spores. These results 
      allowed to refine a model for ff biofilm formation. The overall results help to 
      understand key aspects determining the presence of P. brevicompactum and 
      P. expansum biofilms in DW, where stagnant conditions and the presence of 
      nutrients should be avoided to prevent ff biofilm formation.
CI  - Copyright (c) 2019 Elsevier Ltd. All rights reserved.
FAU - Fernandes, Susana
AU  - Fernandes S
AD  - LEPABE, Faculty of Engineering, Department of Chemical Engineering, University of 
      Porto, Porto, Portugal.
FAU - Simoes, Lucia Chaves
AU  - Simoes LC
AD  - LEPABE, Faculty of Engineering, Department of Chemical Engineering, University of 
      Porto, Porto, Portugal; CEB, Centre of Biological Engineering, University of 
      Minho, Braga, Portugal.
FAU - Lima, Nelson
AU  - Lima N
AD  - CEB, Centre of Biological Engineering, University of Minho, Braga, Portugal.
FAU - Simoes, Manuel
AU  - Simoes M
AD  - LEPABE, Faculty of Engineering, Department of Chemical Engineering, University of 
      Porto, Porto, Portugal. Electronic address: mvs@fe.up.pt.
LA  - eng
PT  - Journal Article
DEP - 20190805
PL  - England
TA  - Water Res
JT  - Water research
JID - 0105072
RN  - 0 (Drinking Water)
SB  - IM
MH  - Biofilms
MH  - *Drinking Water
MH  - Fungi
MH  - Spores, Fungal
MH  - Surface Properties
OTO - NOTNLM
OT  - Abiotic factors
OT  - Adhesion
OT  - Biofilm
OT  - Drinking water
OT  - Filamentous fungi
EDAT- 2019/08/16 06:00
MHDA- 2019/11/20 06:00
CRDT- 2019/08/16 06:00
PHST- 2019/01/04 00:00 [received]
PHST- 2019/07/29 00:00 [revised]
PHST- 2019/08/03 00:00 [accepted]
PHST- 2019/08/16 06:00 [pubmed]
PHST- 2019/11/20 06:00 [medline]
PHST- 2019/08/16 06:00 [entrez]
AID - S0043-1354(19)30725-0 [pii]
AID - 10.1016/j.watres.2019.114951 [doi]
PST - ppublish
SO  - Water Res. 2019 Nov 1;164:114951. doi: 10.1016/j.watres.2019.114951. Epub 2019 
      Aug 5.