PMID- 31417362
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1662-5102 (Print)
IS  - 1662-5102 (Electronic)
IS  - 1662-5102 (Linking)
VI  - 13
DP  - 2019
TI  - Using Genetically Encoded Voltage Indicators (GEVIs) to Study the Input-Output 
      Transformation of the Mammalian Olfactory Bulb.
PG  - 342
LID - 10.3389/fncel.2019.00342 [doi]
LID - 342
AB  - Genetically encoded voltage indicators (GEVIs) are fluorescent protein reporters 
      of membrane potential. These tools can, in principle, be used to monitor the 
      neural activity of genetically distinct cell types in the brain. Although 
      introduced in 1997, they have been a challenge to use to study intact neural 
      circuits due to a combination of small signal-to-noise ratio, slow kinetics, and 
      poor membrane expression. New strategies have yielded novel GEVIs such as 
      ArcLight, which have improved properties. Here, we compare the in vivo properties 
      of ArcLight with Genetically Encoded Calcium Indicators (GECIs) in the mouse 
      olfactory bulb. We show how voltage imaging can be combined with organic calcium 
      sensitive dyes to measure the input-output transformation of the olfactory bulb. 
      Finally, we demonstrate that ArcLight can be targeted to olfactory bulb 
      interneurons. The olfactory bulb contributes substantially to the perception of 
      the concentration invariance of odor recognition.
FAU - Storace, Douglas A
AU  - Storace DA
AD  - Department of Biological Science, Florida State University, Tallahassee, FL, 
      United States.
AD  - Department of Cellular and Molecular Physiology, Yale School of Medicine, Yale 
      University, New Haven, CT, United States.
FAU - Cohen, Lawrence B
AU  - Cohen LB
AD  - Department of Cellular and Molecular Physiology, Yale School of Medicine, Yale 
      University, New Haven, CT, United States.
AD  - Center for Functional Connectomics, Korea Institute of Science and Technology, 
      Seoul, South Korea.
FAU - Choi, Yunsook
AU  - Choi Y
AD  - Department of Cellular and Molecular Physiology, Yale School of Medicine, Yale 
      University, New Haven, CT, United States.
AD  - Center for Functional Connectomics, Korea Institute of Science and Technology, 
      Seoul, South Korea.
LA  - eng
GR  - R01 DC005259/DC/NIDCD NIH HHS/United States
GR  - F32 DC012981/DC/NIDCD NIH HHS/United States
GR  - R21 DC016133/DC/NIDCD NIH HHS/United States
GR  - U01 NS099691/NS/NINDS NIH HHS/United States
GR  - R56 DC005259/DC/NIDCD NIH HHS/United States
PT  - Journal Article
DEP - 20190731
PL  - Switzerland
TA  - Front Cell Neurosci
JT  - Frontiers in cellular neuroscience
JID - 101477935
PMC - PMC6684792
OTO - NOTNLM
OT  - ArcLight
OT  - GECIs
OT  - GEVIs
OT  - mouse
OT  - odors
OT  - olfactory bulb
OT  - perception
EDAT- 2019/08/17 06:00
MHDA- 2019/08/17 06:01
PMCR- 2019/01/01
CRDT- 2019/08/17 06:00
PHST- 2019/03/26 00:00 [received]
PHST- 2019/07/11 00:00 [accepted]
PHST- 2019/08/17 06:00 [entrez]
PHST- 2019/08/17 06:00 [pubmed]
PHST- 2019/08/17 06:01 [medline]
PHST- 2019/01/01 00:00 [pmc-release]
AID - 10.3389/fncel.2019.00342 [doi]
PST - epublish
SO  - Front Cell Neurosci. 2019 Jul 31;13:342. doi: 10.3389/fncel.2019.00342. 
      eCollection 2019.