PMID- 31417520
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 10
DP  - 2019
TI  - Experimental Therapy of Paracoccidioidomycosis Using P10-Primed Monocyte-Derived 
      Dendritic Cells Isolated From Infected Mice.
PG  - 1727
LID - 10.3389/fmicb.2019.01727 [doi]
LID - 1727
AB  - Paracoccidioidomycosis (PCM) is an endemic mycosis in Latin American caused by 
      the thermodimorphic fungi of the genus Paracoccidioides spp. Notably, a Th1 
      immune response is required to control PCM. In this context, dendritic cells 
      (DCs) seem to be essential players in capture, processing and presentation of 
      Paracoccidioides antigens to naive T cells and their further activation. We have 
      previously demonstrated that differentiated DCs from bone marrow cells, pulsed 
      with the immunoprotective peptide 10 (P10), effectively control experimental PCM 
      immunocompetent and immunosuppressed mice. However, this procedure may not be 
      infeasible or it is limited for the therapy of human patients. Therefore, we have 
      sought a less invasive but equally effective approach that would better mimics 
      the autologous transplant of DC in a human patient. Here, we isolated and 
      generated monocyte differentiated dendritic cells (MoDCs) from infected mice, 
      pulsed them with P-10, and used them in the therapy of PCM in syngeneic mice. 
      Similar to the results using BMDCs, the P10-pulsed MoDCs stimulated the 
      proliferation of CD4(+) T lymphocytes, induced a mixed production of Th(1)/Th(2) 
      cytokines and decreased the fungal burden in murine lungs in the setting of PCM. 
      The process of differentiating MoDCs derived from an infected host, and 
      subsequently used for therapy of PCM is much simpler than that for obtaining 
      BMDCs, and represents a more reasonable approach to treat patients infected with 
      Paracoccidioides. The results presented suggest that P10-primed MoDC may be a 
      promising strategy to combat complicated PCM as well as to significantly shorten 
      the lengthy requirements for treatment of patients with this fungal disease.
FAU - Silva, Leandro B R
AU  - Silva LBR
AD  - USP-LIM53, Laboratory of Medical Mycology, Institute of Tropical Medicine, 
      University of Sao Paulo, Sao Paulo, Brazil.
FAU - Taira, Cleison L
AU  - Taira CL
AD  - Department of Microbiology, Institute of Biomedical Sciences, University of Sao 
      Paulo, Sao Paulo, Brazil.
FAU - Dias, Lucas S
AU  - Dias LS
AD  - Department of Microbiology, Institute of Biomedical Sciences, University of Sao 
      Paulo, Sao Paulo, Brazil.
FAU - Souza, Ana C O
AU  - Souza ACO
AD  - Department of Microbiology, Institute of Biomedical Sciences, University of Sao 
      Paulo, Sao Paulo, Brazil.
FAU - Nosanchuk, Joshua D
AU  - Nosanchuk JD
AD  - Departments of Medicine (Division of Infectious Diseases) and Microbiology and 
      Immunology, Albert Einstein College of Medicine, New York, NY, United States.
FAU - Travassos, Luiz R
AU  - Travassos LR
AD  - Department of Microbiology, Immunology and Parasitology, Federal University of 
      Sao Paulo, Sao Paulo, Brazil.
FAU - Taborda, Carlos P
AU  - Taborda CP
AD  - USP-LIM53, Laboratory of Medical Mycology, Institute of Tropical Medicine, 
      University of Sao Paulo, Sao Paulo, Brazil.
AD  - Department of Microbiology, Institute of Biomedical Sciences, University of Sao 
      Paulo, Sao Paulo, Brazil.
LA  - eng
GR  - R01 AI052733/AI/NIAID NIH HHS/United States
GR  - R21 AI124797/AI/NIAID NIH HHS/United States
PT  - Journal Article
DEP - 20190731
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC6685297
OTO - NOTNLM
OT  - dendritic cells
OT  - monocyte-derived dendritic cells
OT  - paracoccidioidomycosis
OT  - peptide P10
OT  - vaccine
EDAT- 2019/08/17 06:00
MHDA- 2019/08/17 06:01
PMCR- 2019/07/31
CRDT- 2019/08/17 06:00
PHST- 2019/04/25 00:00 [received]
PHST- 2019/07/12 00:00 [accepted]
PHST- 2019/08/17 06:00 [entrez]
PHST- 2019/08/17 06:00 [pubmed]
PHST- 2019/08/17 06:01 [medline]
PHST- 2019/07/31 00:00 [pmc-release]
AID - 10.3389/fmicb.2019.01727 [doi]
PST - epublish
SO  - Front Microbiol. 2019 Jul 31;10:1727. doi: 10.3389/fmicb.2019.01727. eCollection 
      2019.