PMID- 31418069
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20191121
LR  - 20200310
IS  - 1436-5073 (Electronic)
IS  - 0026-3672 (Linking)
VI  - 186
IP  - 9
DP  - 2019 Aug 15
TI  - Solid phase extraction and capillary electrophoretic separation of racemic 
      catecholamines by using magnetic particles coated with a copolymer prepared from 
      poly(3,4-dihydroxyphenylalanine) and polyethyleneimine.
PG  - 627
LID - 10.1007/s00604-019-3731-z [doi]
AB  - A method is presented for chiral separation of catecholamines present in bovine 
      and mice blood. It combines magnetic solid phase extraction (MSPE) and chiral 
      capillary electrophoresis (ch-CE). A copolymer consisting of 
      poly(3,4-dihydroxyphenylalanine) and polyethyleneimine was coated onto magnetic 
      particles (MPs) by co-deposition using the CuSO(4)/H(2)O(2) system as a 
      polymerization initiator. The coated MPs are spherical and the average diameter 
      is about 168 +/- 4 nm. The thickness of the coating is approximately 19 nm. The 
      functional MPs are used as sorbents in MSPE to simultaneously extract the 
      catecholamines epinephrine, norepinephrine and isoprenaline. Under the optimum 
      conditions, the extraction efficiencies for those catecholamines are in the range 
      from 92.3 to 98.3%, with relative standard deviations (RSDs) of <5.3%. The 
      extraction can be performed within 4 min. The extracts were then submitted to 
      ch-CE. A method for field-enhanced sample injection (FESI) was used to enhance 
      the detection sensitivities of the enantiomers. The limits of detection for 
      catecholamine enantiomers range from 400 to 600 pg mL(-1). In comparison with the 
      FESI-ch-CE method, the sensitivity enhancement factors of the MSPE/ch-CE method 
      for catecholamines are about 10-fold. The method was applied to the determination 
      of trace levels of catecholamine enantiomers in (spiked) bovine and mice blood. 
      The recoveries ranged from 88.2 to 93.8%, with RSDs of <5.5%. The whole detection 
      procedure takes less than 30 min. Graphical abstract Schematic representation of 
      the separation and detection of catecholamine enantiomers in blood by combination 
      of polyDOPA/PEI-magnetic particles-based solid phase extraction and 
      chiral-capillary electrophoresis.
FAU - Wu, Jia
AU  - Wu J
AD  - Ministry of Education Key Laboratory of Laser Life Science & Institute of Laser 
      Life Science, College of Biophotonics, South China Normal University, Guangzhou, 
      510631, China.
FAU - Li, Zhenqun
AU  - Li Z
AD  - Ministry of Education Key Laboratory of Laser Life Science & Institute of Laser 
      Life Science, College of Biophotonics, South China Normal University, Guangzhou, 
      510631, China.
FAU - Jia, Li
AU  - Jia L
AUID- ORCID: 0000-0002-1979-5128
AD  - Ministry of Education Key Laboratory of Laser Life Science & Institute of Laser 
      Life Science, College of Biophotonics, South China Normal University, Guangzhou, 
      510631, China. jiali@scnu.edu.cn.
LA  - eng
GR  - 21675056/National Natural Science Foundation of China/International
GR  - 201805010002/Scientific and Technological Planning Project of Guangzhou 
      City/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190815
PL  - Austria
TA  - Mikrochim Acta
JT  - Mikrochimica acta
JID - 7808782
SB  - IM
OTO - NOTNLM
OT  - Blood
OT  - Chiral capillary electrophoresis
OT  - Chiral pharmaceuticals
OT  - Enantioseparation
OT  - Magnetic separation
OT  - On-line concentration
EDAT- 2019/08/17 06:00
MHDA- 2019/08/17 06:01
CRDT- 2019/08/17 06:00
PHST- 2019/04/01 00:00 [received]
PHST- 2019/08/01 00:00 [accepted]
PHST- 2019/08/17 06:00 [entrez]
PHST- 2019/08/17 06:00 [pubmed]
PHST- 2019/08/17 06:01 [medline]
AID - 10.1007/s00604-019-3731-z [pii]
AID - 10.1007/s00604-019-3731-z [doi]
PST - epublish
SO  - Mikrochim Acta. 2019 Aug 15;186(9):627. doi: 10.1007/s00604-019-3731-z.