PMID- 31418686
OWN - NLM
STAT- MEDLINE
DCOM- 20200219
LR  - 20200422
IS  - 2050-084X (Electronic)
IS  - 2050-084X (Linking)
VI  - 8
DP  - 2019 Aug 16
TI  - Epigenetic silencing of a multifunctional plant stress regulator.
LID - 10.7554/eLife.47835 [doi]
LID - e47835
AB  - The central regulator of the ethylene (ET) signaling pathway, which controls a 
      plethora of developmental programs and responses to environmental cues in plants, 
      is ETHYLENE-INSENSITIVE2 (EIN2). Here we identify a chromatin-dependent 
      regulatory mechanism at EIN2 requiring two genes: ETHYLENE-INSENSITIVE6 (EIN6), 
      which is a H3K27me3 demethylase also known as RELATIVE OF EARLY FLOWERING6 
      (REF6), and EIN6 ENHANCER (EEN), the Arabidopsis homolog of the yeast INO80 
      chromatin remodeling complex subunit IES6 (INO EIGHTY SUBUNIT). Strikingly, EIN6 
      (REF6) and the INO80 complex redundantly control the level and the localization 
      of the repressive histone modification H3K27me3 and the histone variant H2A.Z at 
      the 5' untranslated region (5'UTR) intron of EIN2. Concomitant loss of EIN6 
      (REF6) and the INO80 complex shifts the chromatin landscape at EIN2 to a 
      repressive state causing a dramatic reduction of EIN2 expression. These results 
      uncover a unique type of chromatin regulation which safeguards the expression of 
      an essential multifunctional plant stress regulator.
CI  - (c) 2019, Zander et al.
FAU - Zander, Mark
AU  - Zander M
AUID- ORCID: 0000-0001-8643-1407
AD  - Plant Biology Laboratory, Salk Institute for Biological Studies, La Jolla, United 
      States.
AD  - Genomic Analysis Laboratory, Salk Institute for Biological Studies, La Jolla, 
      United States.
AD  - Howard Hughes Medical Institute, Salk Institute for Biological Studies, La Jolla, 
      United States.
FAU - Willige, Bjorn C
AU  - Willige BC
AUID- ORCID: 0000-0003-4275-5826
AD  - Plant Biology Laboratory, Salk Institute for Biological Studies, La Jolla, United 
      States.
FAU - He, Yupeng
AU  - He Y
AUID- ORCID: 0000-0001-9313-614X
AD  - Genomic Analysis Laboratory, Salk Institute for Biological Studies, La Jolla, 
      United States.
FAU - Nguyen, Thu A
AU  - Nguyen TA
AD  - Plant Biology Laboratory, Salk Institute for Biological Studies, La Jolla, United 
      States.
FAU - Langford, Amber E
AU  - Langford AE
AUID- ORCID: 0000-0001-9573-9905
AD  - Plant Biology Laboratory, Salk Institute for Biological Studies, La Jolla, United 
      States.
FAU - Nehring, Ramlah
AU  - Nehring R
AD  - Plant Biology Laboratory, Salk Institute for Biological Studies, La Jolla, United 
      States.
FAU - Howell, Elizabeth
AU  - Howell E
AD  - Plant Biology Laboratory, Salk Institute for Biological Studies, La Jolla, United 
      States.
FAU - McGrath, Robert
AU  - McGrath R
AUID- ORCID: 0000-0002-4002-1522
AD  - Department of Biology, University of Pennsylvania, Philadelphia, United States.
FAU - Bartlett, Anna
AU  - Bartlett A
AD  - Genomic Analysis Laboratory, Salk Institute for Biological Studies, La Jolla, 
      United States.
FAU - Castanon, Rosa
AU  - Castanon R
AD  - Genomic Analysis Laboratory, Salk Institute for Biological Studies, La Jolla, 
      United States.
FAU - Nery, Joseph R
AU  - Nery JR
AD  - Genomic Analysis Laboratory, Salk Institute for Biological Studies, La Jolla, 
      United States.
FAU - Chen, Huaming
AU  - Chen H
AUID- ORCID: 0000-0001-5289-7882
AD  - Genomic Analysis Laboratory, Salk Institute for Biological Studies, La Jolla, 
      United States.
FAU - Zhang, Zhuzhu
AU  - Zhang Z
AD  - Genomic Analysis Laboratory, Salk Institute for Biological Studies, La Jolla, 
      United States.
FAU - Jupe, Florian
AU  - Jupe F
AUID- ORCID: 0000-0001-5741-4931
AD  - Genomic Analysis Laboratory, Salk Institute for Biological Studies, La Jolla, 
      United States.
FAU - Stepanova, Anna
AU  - Stepanova A
AD  - Plant Biology Laboratory, Salk Institute for Biological Studies, La Jolla, United 
      States.
FAU - Schmitz, Robert J
AU  - Schmitz RJ
AUID- ORCID: 0000-0001-7538-6663
AD  - Plant Biology Laboratory, Salk Institute for Biological Studies, La Jolla, United 
      States.
FAU - Lewsey, Mathew G
AU  - Lewsey MG
AUID- ORCID: 0000-0002-2631-4337
AD  - Plant Biology Laboratory, Salk Institute for Biological Studies, La Jolla, United 
      States.
FAU - Chory, Joanne
AU  - Chory J
AD  - Plant Biology Laboratory, Salk Institute for Biological Studies, La Jolla, United 
      States.
AD  - Howard Hughes Medical Institute, Salk Institute for Biological Studies, La Jolla, 
      United States.
FAU - Ecker, Joseph R
AU  - Ecker JR
AUID- ORCID: 0000-0001-5799-5895
AD  - Plant Biology Laboratory, Salk Institute for Biological Studies, La Jolla, United 
      States.
AD  - Genomic Analysis Laboratory, Salk Institute for Biological Studies, La Jolla, 
      United States.
AD  - Howard Hughes Medical Institute, Salk Institute for Biological Studies, La Jolla, 
      United States.
LA  - eng
SI  - GEO/GSE122314
GR  - R35 GM122604/GM/NIGMS NIH HHS/United States
GR  - MCB-1024999/National Science Foundation/International
GR  - DE-FG02-04ER15517/Department of Energy, Office of Basic Energy 
      Sciences/International
GR  - EU Marie Curie FP7 International Outgoing Fellowship: 252475/EU 7th Framework 
      Programme/International
GR  - GBMF3034/Gordon and Betty Moore Foundation/International
GR  - LT000222/2013-L/Human Frontier Science Program/International
GR  - DOE FG02 04ER15517/U.S. Department of Energy/International
GR  - Za-730/1-1/Deutsche Forschungsgemeinschaft/International
GR  - 5R35 GM122604/NH/NIH HHS/United States
GR  - 252475/EU Marie Curie FP7 International Outgoing Fellowship/International
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20190816
PL  - England
TA  - Elife
JT  - eLife
JID - 101579614
RN  - 0 (AT4G38495 protein, Arabidopsis)
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (EIN2 protein, Arabidopsis)
RN  - 0 (Ethylenes)
RN  - 0 (Nuclear Proteins)
RN  - 0 (REF6 protein, Arabidopsis)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Transcription Factors)
RN  - 91GW059KN7 (ethylene)
SB  - IM
MH  - Arabidopsis/*physiology
MH  - Arabidopsis Proteins/*biosynthesis/*metabolism
MH  - *Epigenesis, Genetic
MH  - Ethylenes/metabolism
MH  - *Gene Expression Regulation, Plant
MH  - Nuclear Proteins/*metabolism
MH  - Receptors, Cell Surface/*biosynthesis
MH  - Signal Transduction
MH  - Transcription Factors/*metabolism
PMC - PMC6739875
OTO - NOTNLM
OT  - A. thaliana
OT  - chromatin remodeling
OT  - ethylene
OT  - genetics
OT  - genomics
OT  - histone demethylation
OT  - plant biology
OT  - plant epigenomics
COIS- MZ, BW, YH, TN, AL, RN, EH, RM, AB, RC, JN, HC, ZZ, FJ, AS, RS, ML, JC, JE No 
      competing interests declared
EDAT- 2019/08/17 06:00
MHDA- 2020/02/20 06:00
PMCR- 2019/08/16
CRDT- 2019/08/17 06:00
PHST- 2019/04/20 00:00 [received]
PHST- 2019/08/15 00:00 [accepted]
PHST- 2019/08/17 06:00 [pubmed]
PHST- 2020/02/20 06:00 [medline]
PHST- 2019/08/17 06:00 [entrez]
PHST- 2019/08/16 00:00 [pmc-release]
AID - 47835 [pii]
AID - 10.7554/eLife.47835 [doi]
PST - epublish
SO  - Elife. 2019 Aug 16;8:e47835. doi: 10.7554/eLife.47835.