PMID- 31420628
OWN - NLM
STAT- MEDLINE
DCOM- 20190822
LR  - 20200917
IS  - 1671-167X (Print)
IS  - 1671-167X (Linking)
VI  - 51
IP  - 4
DP  - 2019 Aug 18
TI  - [Altered serum cytokine expression profile in systemic sclerosis and its 
      regulatory mechanisms].
PG  - 716-722
LID - 10.19723/j.issn.1671-167X.2019.04.021 [doi]
AB  - OBJECTIVE: To analyze the expression profile of serum cytokines in patients with 
      systemic sclerosis (SSc) and explore its possible regulatory mechanisms. METHODS: 
      Serum and DNA of peripheral blood mononuclear cells were collected from 30 SSc 
      patients and 80 normal controls (NCs). According to the presence or absence of 
      interstitial lung disease (ILD) in SSc, the patients were divided into SSc with 
      ILD group and SSc without ILD group. According to the degree of skin involvement, 
      the patients were divided into diffuse systemic scleroderma (dcSSc) group and 
      limited systemic scleroderma (lcSSc) group. According to the presence of 
      anti-topoisomerase-1 antibody (anti-Scl-70 antibody) in the serum of patients 
      with SSc, they were divided into SSc Scl-70 (+) group and SSc Scl-70 (-) group. 
      27 cytokines in serum were detected by Luminex MAGPIX detection system and 
      Bio-Plex Pro Human Cytokine 27-plex Assay kit: interleukin-1beta (IL-1beta), 
      interleukin-1 receptor antagonist (IL-1ra), IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, 
      IL-9, IL-10, IL-12P70, IL-13, IL-15, IL-17, basic fiber growth factor (BASIC 
      FGF), eotaxin, granulocyte colony stimulating factor (G-CSF), 
      granulocyte-macrophage colony stimulating factor (GM-CSF), interferon-gamma (IFN-gamma), 
      interferon-gamma induced protein 10(IP-10), monocyte chemotactic protein 
      1(MCP-1), macrophage inflammatory protein-1alpha (MIP-1alpha), macrophage inflammatory 
      protein 1beta(MIP-1beta), platelet-derived growth factor BB (PDGF-BB), regulated on 
      activation in normal T-cell expressed and secreted (RANTES), tumor necrosis 
      factor-alpha (TNF-alpha), and vascular endothelial growth factor(VEGF). Methylation sites 
      were detected by Illumina 450K methylation chip. RESULTS: Compared with NCs 
      group, the expression of 12 cytokines (BASIC FGF, eotaxin, G-CSF, GM-CSF, IFN-gamma, 
      IL-1beta, IL-1ra, IL-6, IP-10, MCP-1, TNF-alpha and RANTES) in the SSc group 
      significantly increased (P<0.05), IL-5 was decreased expression in the SSc group 
      (P<0.05), there was no significant difference in the expressions of the other 14 
      cytokines. Compared with lcSSc group, 9 cytokines (eotaxin, IL-5, MCP-1, IL-2, 
      RANTES, IL17A, IL-8, MIP-1beta and PDGF-BB) increased in dcSSc group, but there was 
      no significant difference. Compared with SSc without ILD group, IL-15 increased 
      in SSC with ILD group [18.2 (172.97) ng/L vs. 2.03(0.05) ng/L, P<0.05]. Compared 
      with SSc Scl-70 (-) group, the expression of IP-10 decreased in SSc Scl-70 (+) 
      group [1 030 (2 196.6) ng/L vs. 1 878 (2 964) ng/L, P<0.05]. The correlation 
      analysis of serum cytokines with erythrocyte sedimentation rate (ESR) and 
      C-reactive protein (CRP) showed that IL-6 was positively correlated with ESR (r 
      =0.04, P= 0.017), MCP-1 (r=0.49, P=0.043) and MIP-1beta (r=0.41, P=0.007) positively 
      correlated with CRP. By analyzing the changes of methylation sites of cytokines, 
      it was found that cg17744604 in IL-10 TSS1500 region, cg06111286 in IL-12P70 
      TSS200 region, cg07935264 in IL-1beta TSS200 region, cg01467417 in IL-1ra TSS1500 
      region, cg03989987 in IL-1ra 5'UTR region and cg21099624 in VEGF TSS200 region 
      were all hypomethylated. CONCLUSION: There were different cytokines expression 
      profiles in the serum of SSc patients, and the altered cytokines were correlected 
      with the degree of skin damage and pulmonary fibrosis. Many cytokines were 
      regulated by methylation.
FAU - Zhu, H L
AU  - Zhu HL
AD  - Department of Rheumatology and Immunology, Xiangya Hospital, Central South 
      University, Changsha 410008, China.
FAU - DU, Q
AU  - DU Q
AD  - Department of Rheumatology and Immunology, Xiangya Hospital, Central South 
      University, Changsha 410008, China.
FAU - Chen, W L
AU  - Chen WL
AD  - Department of Rheumatology and Immunology, Xiangya Hospital, Central South 
      University, Changsha 410008, China.
FAU - Zuo, X X
AU  - Zuo XX
AD  - Department of Rheumatology and Immunology, Xiangya Hospital, Central South 
      University, Changsha 410008, China.
FAU - Li, Q Z
AU  - Li QZ
AD  - Department of Rheumatology and Immunology, Xiangya Hospital, Central South 
      University, Changsha 410008, China.
FAU - Liu, S J
AU  - Liu SJ
AD  - Department of Rheumatology and Immunology, Xiangya Hospital, Central South 
      University, Changsha 410008, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Beijing Da Xue Xue Bao Yi Xue Ban
JT  - Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health 
      sciences
JID - 101125284
RN  - 0 (Cytokines)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Cytokines
MH  - Humans
MH  - *Leukocytes, Mononuclear
MH  - *Scleroderma, Systemic
MH  - Tumor Necrosis Factor-alpha
MH  - Vascular Endothelial Growth Factor A
PMC - PMC7433485
EDAT- 2019/08/20 06:00
MHDA- 2019/08/23 06:00
PMCR- 2019/08/18
CRDT- 2019/08/18 06:00
PHST- 2019/08/18 06:00 [entrez]
PHST- 2019/08/20 06:00 [pubmed]
PHST- 2019/08/23 06:00 [medline]
PHST- 2019/08/18 00:00 [pmc-release]
AID - bjdxxbyxb-51-4-716 [pii]
AID - 10.19723/j.issn.1671-167X.2019.04.021 [doi]
PST - ppublish
SO  - Beijing Da Xue Xue Bao Yi Xue Ban. 2019 Aug 18;51(4):716-722. doi: 
      10.19723/j.issn.1671-167X.2019.04.021.