PMID- 31422500
OWN - NLM
STAT- MEDLINE
DCOM- 20200601
LR  - 20200601
IS  - 1573-3890 (Electronic)
IS  - 1573-3882 (Linking)
VI  - 15
IP  - 8
DP  - 2019 Aug 17
TI  - Metabolomic study on bleomycin and polyhexamethylene guanidine phosphate-induced 
      pulmonary fibrosis mice models.
PG  - 111
LID - 10.1007/s11306-019-1574-6 [doi]
AB  - INTRODUCTION: Polyhexamethylene guanidine phosphate (PHMG) has been used as a 
      disinfectant and biocide, and was known to be harmless and non-toxic. However, in 
      2011, PHMG used as a humidifier disinfectant was reported to be associated with 
      lung diseases, such as, fibrosis in the toxicant studies on pulmonary fibrosis by 
      PHMG. However, no metabolomics study has been performed in PHMG-induced mouse 
      models of pulmonary fibrosis. OBJECTIVES: We performed a metabolomic study to 
      understand the biochemical events that occur in bleomycin (BLM)- and PHMG-induced 
      mouse models of pulmonary fibrosis using gas chromatography-mass spectrometry 
      (GC-MS), LC-tandem MS, and GC-tandem MS. RESULTS: The levels of 61 metabolites of 
      30 amino acids, 13 organic acids, 12 fatty acids, 5 polyamines, and oxidized 
      glutathione were determined in the pulmonary tissues of mice with BLM- and 
      PHMG-induced pulmonary fibrosis and in normal controls. Principal component 
      analysis and partial least squares discriminant analysis used to compare level of 
      these 61 metabolites in pulmonary tissues. Levels of metabolites were 
      significantly different in the BLM and PHMG groups as compared with the control 
      group. In particular, the BLM- and PHMG-induced pulmonary fibrosis models showed 
      elevated collagen synthesis and oxidative stress and metabolic disturbance of TCA 
      related organic acids including fumaric acid by NADPH oxidase. In addition, 
      polyamine metabolism showed severe alteration in the PHMG group than that of the 
      BLM group. CONCLUSION: This result suggests PHMG will be able to induce pulmonary 
      fibrosis by arginine metabolism and NADPH oxidase signaling.
FAU - Seo, Chan
AU  - Seo C
AD  - College of Pharmacy, Sunchon National University, Suncheon, 540-950, Republic of 
      Korea.
FAU - Kim, Sung-Hwan
AU  - Kim SH
AD  - National Center for Efficacy Evaluation of Respiratory Disease Product, Institute 
      of Toxicology, Jeongeup-si, 56212, Republic of Korea.
FAU - Lee, Hyeon-Seong
AU  - Lee HS
AD  - College of Pharmacy, Sunchon National University, Suncheon, 540-950, Republic of 
      Korea.
FAU - Ji, Moongi
AU  - Ji M
AD  - College of Pharmacy, Sunchon National University, Suncheon, 540-950, Republic of 
      Korea.
FAU - Min, Jeuk
AU  - Min J
AD  - College of Pharmacy, Sunchon National University, Suncheon, 540-950, Republic of 
      Korea.
FAU - Son, Young-Jin
AU  - Son YJ
AD  - College of Pharmacy, Sunchon National University, Suncheon, 540-950, Republic of 
      Korea.
FAU - Kim, In-Hyeon
AU  - Kim IH
AD  - National Center for Efficacy Evaluation of Respiratory Disease Product, Institute 
      of Toxicology, Jeongeup-si, 56212, Republic of Korea.
FAU - Lee, Kyuhong
AU  - Lee K
AD  - National Center for Efficacy Evaluation of Respiratory Disease Product, Institute 
      of Toxicology, Jeongeup-si, 56212, Republic of Korea. khleekit@gmail.com.
FAU - Paik, Man-Jeong
AU  - Paik MJ
AUID- ORCID: 0000-0003-1654-3133
AD  - College of Pharmacy, Sunchon National University, Suncheon, 540-950, Republic of 
      Korea. paik815@sunchon.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190817
PL  - United States
TA  - Metabolomics
JT  - Metabolomics : Official journal of the Metabolomic Society
JID - 101274889
RN  - 0 (Guanidines)
RN  - 11056-06-7 (Bleomycin)
RN  - 31961-54-3 (polyhexamethyleneguanidine)
SB  - IM
MH  - Animals
MH  - Bleomycin/administration & dosage/analysis/*metabolism
MH  - Chromatography, Gas
MH  - Chromatography, Liquid
MH  - *Disease Models, Animal
MH  - Guanidines/administration & dosage/analysis/*metabolism
MH  - Male
MH  - *Metabolomics
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Pulmonary Fibrosis/*chemically induced/*metabolism/pathology
MH  - Tandem Mass Spectrometry
OTO - NOTNLM
OT  - Bleomycin
OT  - Metabolic profiling analysis
OT  - Metabolomics
OT  - Polyhexamethylene guanidine phosphate
OT  - Pulmonary fibrosis
OT  - Tandem mass spectrometry
EDAT- 2019/08/20 06:00
MHDA- 2020/06/02 06:00
CRDT- 2019/08/19 06:00
PHST- 2019/01/22 00:00 [received]
PHST- 2019/08/01 00:00 [accepted]
PHST- 2019/08/19 06:00 [entrez]
PHST- 2019/08/20 06:00 [pubmed]
PHST- 2020/06/02 06:00 [medline]
AID - 10.1007/s11306-019-1574-6 [pii]
AID - 10.1007/s11306-019-1574-6 [doi]
PST - epublish
SO  - Metabolomics. 2019 Aug 17;15(8):111. doi: 10.1007/s11306-019-1574-6.