PMID- 31423645
OWN - NLM
STAT- MEDLINE
DCOM- 20200423
LR  - 20200423
IS  - 1439-0396 (Electronic)
IS  - 0931-2439 (Linking)
VI  - 103
IP  - 6
DP  - 2019 Nov
TI  - Deoxynivalenol induces oxidative stress, inflammatory response and apoptosis in 
      bovine mammary epithelial cells.
PG  - 1663-1674
LID - 10.1111/jpn.13180 [doi]
AB  - Deoxynivalenol (DON) is a toxic secondary metabolite produced by Fusarium 
      graminearum. It is one of the most common feed contaminants that poses a serious 
      threat to the health and performance of dairy cows. This study investigated the 
      in vitro cytotoxicity of DON on bovine mammary epithelial cells (MAC-T). DON at 
      different concentrations (0.25, 0.3, 0.5, 0.8, 1 or 2 mug/ml) inhibited the growth 
      of MAC-T cells after 24 hr of exposure (p < .001). DON at 0.25 mug/ml increased 
      lactate dehydrogenase (LDH) leakage (p < .05); decreased glutathione (GSH) levels 
      (p < .001), total superoxide dismutase (T-SOD) activity and total antioxidant 
      capacity (T-AOC; p < .01); and increased malondialdehyde (MDA) concentration 
      (p < .01) in MAC-T cells after 24 hr of exposure. We also observed that DON 
      increased reactive oxygen species (ROS) levels in cells incubated for 9, 15 and 
      24 hr (p < .001). DON at 0.25 mug/ml triggered oxidative damage in MAC-T cells. 
      Furthermore, it induced an inflammatory response in the cells incubated for 9, 15 
      and 24 hr (p < .05) by increasing the mRNA expression levels of nuclear factor 
      kappa B, myeloid differentiation factor 88 (MyD88), tumour necrosis factor-alpha 
      (TNF-alpha), interleukin-1beta (IL-1beta), IL-6, cyclooxygenase-2 and IL-8. We further 
      examined the effect of DON on apoptosis. DON prevented normal proliferation of 
      MAC-T cells by blocked cell cycle progression in 24 hr (p < .001). In addition, 
      the apoptosis rate measured using annexin V-FITC significantly increased 
      (p < .05) with increase in the mRNA expression level of Bax (p < .01) and 
      increase in the Bax/Bcl-2 ratio (p < .01) in cells incubated for 24 hr. In 
      summary, DON exerts toxic effects in MAC-T cells by causing oxidative stress, 
      inducing an inflammatory response, affecting cell cycle and leading to apoptosis.
CI  - (c) 2019 The Authors. Journal of Animal Physiology and Animal Nutrition Published 
      by Blackwell Verlag GmbH.
FAU - Wang, Junmei
AU  - Wang J
AD  - College of Animal Science, Jilin University, Changchun, China.
FAU - Jin, Yongcheng
AU  - Jin Y
AD  - College of Animal Science, Jilin University, Changchun, China.
FAU - Wu, Shunlu
AU  - Wu S
AD  - College of Animal Science, Jilin University, Changchun, China.
FAU - Yu, Hao
AU  - Yu H
AD  - College of Animal Science, Jilin University, Changchun, China.
FAU - Zhao, Yun
AU  - Zhao Y
AD  - College of Animal Science, Jilin University, Changchun, China.
FAU - Fang, Hengtong
AU  - Fang H
AD  - College of Animal Science, Jilin University, Changchun, China.
FAU - Shen, Jinglin
AU  - Shen J
AD  - College of Animal Science, Jilin University, Changchun, China.
FAU - Zhou, Changhai
AU  - Zhou C
AD  - College of Animal Science, Jilin University, Changchun, China.
FAU - Fu, Yurong
AU  - Fu Y
AD  - College of Animal Science, Jilin University, Changchun, China.
FAU - Li, Ruihua
AU  - Li R
AD  - College of Animal Science, Jilin University, Changchun, China.
FAU - Wang, Rui
AU  - Wang R
AUID- ORCID: 0000-0003-3454-7082
AD  - College of Animal Science, Jilin University, Changchun, China.
FAU - Wang, Junxiong
AU  - Wang J
AD  - College of Animal Science, Jilin University, Changchun, China.
FAU - Zheng, Kexin
AU  - Zheng K
AD  - College of Animal Science, Jilin University, Changchun, China.
FAU - Fan, Qingsong
AU  - Fan Q
AD  - College of Animal Science, Jilin University, Changchun, China.
FAU - Chen, Bojiong
AU  - Chen B
AD  - College of Animal Science, Jilin University, Changchun, China.
FAU - Zhang, Jing
AU  - Zhang J
AUID- ORCID: 0000-0002-4823-4629
AD  - College of Animal Science, Jilin University, Changchun, China.
LA  - eng
SI  - GENBANK/NM_173979.3
SI  - GENBANK/NM_001076409.1
SI  - GENBANK/NM_001080242.2
SI  - GENBANK/NM_174445.2
SI  - GENBANK/NM_173923.2
SI  - GENBANK/NM_173925.2
SI  - GENBANK/NM_174093.1
SI  - GENBANK/NM_001014382.2
SI  - GENBANK/NM_173966.3
SI  - GENBANK/NM_173894.1
SI  - GENBANK/NM_001166486.1
GR  - SXGJXX2017-4/the Special Project of the Province-University Co-constructing 
      Program of Jilin Province/
GR  - the Jilin Modern Agricultural Technology Demonstration and Extension Project, 
      P.R. China./
GR  - 20170307018NY/the Jilin Scientific and Technological Development Program/
GR  - 20190301034NY/the Jilin Scientific and Technological Development Program/
PT  - Journal Article
DEP - 20190818
PL  - Germany
TA  - J Anim Physiol Anim Nutr (Berl)
JT  - Journal of animal physiology and animal nutrition
JID - 101126979
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Annexin A5)
RN  - 0 (Antioxidants)
RN  - 0 (FITC-annexin A5)
RN  - 0 (GluB protein, Corynebacterium glutamicum)
RN  - 0 (Periplasmic Binding Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Trichothecenes)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - I223NX31W9 (Fluorescein-5-isothiocyanate)
RN  - JT37HYP23V (deoxynivalenol)
SB  - IM
MH  - ATP-Binding Cassette Transporters/metabolism
MH  - Animals
MH  - Annexin A5/metabolism
MH  - Antioxidants/metabolism
MH  - Apoptosis/*drug effects
MH  - *Cattle
MH  - Cell Cycle/drug effects
MH  - Cell Line
MH  - Cell Survival
MH  - Epithelial Cells/*drug effects/physiology
MH  - Female
MH  - Fluorescein-5-isothiocyanate/analogs & derivatives/metabolism
MH  - Gene Expression Regulation/drug effects
MH  - Inflammation/chemically induced/*veterinary
MH  - Malondialdehyde/metabolism
MH  - Mammary Glands, Animal
MH  - Oxidative Stress/*drug effects
MH  - Periplasmic Binding Proteins/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Superoxide Dismutase/metabolism
MH  - Trichothecenes/*pharmacology
OTO - NOTNLM
OT  - apoptosis
OT  - bovine mammary epithelial cells
OT  - deoxynivalenol
OT  - inflammation
OT  - oxidative stress
EDAT- 2019/08/20 06:00
MHDA- 2020/04/24 06:00
CRDT- 2019/08/20 06:00
PHST- 2019/05/14 00:00 [received]
PHST- 2019/07/05 00:00 [revised]
PHST- 2019/07/16 00:00 [accepted]
PHST- 2019/08/20 06:00 [pubmed]
PHST- 2020/04/24 06:00 [medline]
PHST- 2019/08/20 06:00 [entrez]
AID - 10.1111/jpn.13180 [doi]
PST - ppublish
SO  - J Anim Physiol Anim Nutr (Berl). 2019 Nov;103(6):1663-1674. doi: 
      10.1111/jpn.13180. Epub 2019 Aug 18.