PMID- 31424653
OWN - NLM
STAT- MEDLINE
DCOM- 20200302
LR  - 20200302
IS  - 2241-6293 (Electronic)
IS  - 1107-0625 (Linking)
VI  - 24
IP  - 3
DP  - 2019 May-Jun
TI  - Scopoletin exerts anticancer effects on human cervical cancer cell lines by 
      triggering apoptosis, cell cycle arrest, inhibition of cell invasion and PI3K/AKT 
      signalling pathway.
PG  - 997-1002
AB  - PURPOSE: Cervical cancer causes considerable mortality in women world over and 
      the current treatment options create severe adverse effects. Hence, there is an 
      urgent need to develop novel and efficient treatment regimens for cervical 
      cancer. Herein, we examined the anticancer effects of a natural coumarin, 
      Scopoletin, against a panel of cervical cancer cell lines. METHODS: The 
      antiproliferative effect of Scopoletin was examined by cell counting and colony 
      formation assays. Apoptosis was detected by acridine orange (AO) ethidium promide 
      (EB) staining. Cell cycle distribution was determined by flow cytometry. Cell 
      invasion was examined by Boyden chamber assay. Protein expression was checked by 
      western blotting. RESULTS: Scopoletin inhibited the growth of all the cell lines 
      and the IC50 ranged between 7.5 to 25 microM. Nonetheless, the cytotoxic effects of 
      Scopoletin were comparatively negligible against the normal cells with an IC50 of 
      90 microM. Investigation of the mechanism of action, revealed that the anticancer 
      effects of Scopoletin against the HeLa cervical cancer cells were due to 
      induction of apoptotic cell death as indicated AO/EB staining. Scopoletin 
      treatment also resulted in enhancement of the Bax, Caspase 3, 8 and 9 expression 
      and decline of the Bcl-2 expression. Scopoletin also blocked the HeLa cells at 
      G2/M checkpoint of the cell cycle. Furthermore, cell invasion assay revealed that 
      Scopoletin inhibited the migration of the HeLa cells concentration-dependently. 
      PI3K/AKT is an imperative pathway involved in theproliferation and tumorigenesis 
      of cancer cells and herein it was found that Scopoletin could inhibit this 
      pathway. CONCLUSION: Taken together, Scopoletin may prove essential in the 
      development of novel treatment regimes for cervical cancer.
FAU - Tian, Qi
AU  - Tian Q
AD  - Department of Obstetrics and Gynecology, The Third Xiangya Hospital of Central 
      South University, Changsha, Hunan, 410013, China.
FAU - Wang, Luying
AU  - Wang L
FAU - Sun, Xin
AU  - Sun X
FAU - Zeng, Fei
AU  - Zeng F
FAU - Pan, Qiong
AU  - Pan Q
FAU - Xue, Min
AU  - Xue M
LA  - eng
PT  - Journal Article
PL  - Cyprus
TA  - J BUON
JT  - Journal of B.U.ON. : official journal of the Balkan Union of Oncology
JID - 100883428
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - KLF1HS0SXJ (Scopoletin)
SB  - IM
MH  - Apoptosis
MH  - Cell Cycle Checkpoints/*genetics
MH  - Female
MH  - Humans
MH  - Phosphatidylinositol 3-Kinases/*metabolism
MH  - Proto-Oncogene Proteins c-akt/*metabolism
MH  - Scopoletin/pharmacology/*therapeutic use
MH  - Signal Transduction
MH  - Uterine Cervical Neoplasms/*drug therapy/genetics
EDAT- 2019/08/20 06:00
MHDA- 2020/03/03 06:00
CRDT- 2019/08/20 06:00
PHST- 2019/08/20 06:00 [entrez]
PHST- 2019/08/20 06:00 [pubmed]
PHST- 2020/03/03 06:00 [medline]
PST - ppublish
SO  - J BUON. 2019 May-Jun;24(3):997-1002.