PMID- 31424663
OWN - NLM
STAT- MEDLINE
DCOM- 20200317
LR  - 20200317
IS  - 2241-6293 (Electronic)
IS  - 1107-0625 (Linking)
VI  - 24
IP  - 3
DP  - 2019 May-Jun
TI  - MicroRNA-155regulates the proliferation and metastasis of human breast cancers by 
      targeting MAPK7.
PG  - 1075-1080
AB  - PURPOSE: Breast cancer causes significant mortality in women world over. The lack 
      of efficient and reliable biomarkers and therapeutic targets impedes the 
      treatment of breast cancer. Herein, the role and therapeutic potential of miR-155 
      was investigated in different breast cancer cell lines Methods: Cell viability 
      was determined by WST-1 and colony formation assays. Transfections were performed 
      by Lipofectamine 2000 reagent. Cell cycle analysis was carried out by flow 
      cytometry and apoptosis was detected by AO (acridine orange)/EB (ethidium 
      bromide) staining. Cell migration and cell invasion were determined by wound 
      healing assay. RNA and protein expressions were determined by qRT-PCR and western 
      blotting, respectively. RESULTS: miR-155 was significantly upregulated in all the 
      breast cancer cells. Suppression of miR-155 in SK-BR-3 cells inhibited the growth 
      and colony formation. The inhibition of SK-BR-3 cell proliferation was found to 
      trigger apoptotic cell death and cell cycle arrest. Induction of apoptosis was 
      also accompanied with enhancement of cytochrome c, Bax caspase 3, 8 and 9and 
      inhibition of Bcl-2. Besides, suppression of miR-155 resulted in the decrease of 
      cell migration and invasion. Bioinformatic analysis revealed MAPK7 to be the 
      potential target of miR-155. The MAPK7 expression was also upregulated in all the 
      breast cancer cells and suppression of miR-155 resulted in its downregulation. 
      CONCLUSION: Taken together, miR-155 may prove essential in the management of 
      breast cancer.
FAU - Liu, Jian-Hua
AU  - Liu JH
AD  - Department of Breast and Thyroid Surgery, Wuhan No.1 Hospital(Wuhan Chinese and 
      Western Medicine Hospital),Wuhan,Hubei,430022,China.
FAU - Yang, Yong
AU  - Yang Y
FAU - Song, Qi
AU  - Song Q
FAU - Li, Jie Bao
AU  - Li JB
LA  - eng
PT  - Journal Article
PL  - Cyprus
TA  - J BUON
JT  - Journal of B.U.ON. : official journal of the Balkan Union of Oncology
JID - 100883428
RN  - 0 (MIRN155 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - EC 2.7.11.24 (MAPK7 protein, human)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 7)
SB  - IM
MH  - Apoptosis/physiology
MH  - Breast Neoplasms/*enzymology/*genetics/pathology
MH  - Cell Cycle Checkpoints/physiology
MH  - Cell Line, Tumor
MH  - Cell Proliferation/physiology
MH  - Female
MH  - Humans
MH  - MCF-7 Cells
MH  - MicroRNAs/antagonists & inhibitors/biosynthesis/*metabolism
MH  - Mitogen-Activated Protein Kinase 7/*genetics/*metabolism
MH  - Neoplasm Metastasis
MH  - Transfection
EDAT- 2019/08/20 06:00
MHDA- 2020/03/18 06:00
CRDT- 2019/08/20 06:00
PHST- 2019/08/20 06:00 [entrez]
PHST- 2019/08/20 06:00 [pubmed]
PHST- 2020/03/18 06:00 [medline]
PST - ppublish
SO  - J BUON. 2019 May-Jun;24(3):1075-1080.