PMID- 31424673
OWN - NLM
STAT- MEDLINE
DCOM- 20200317
LR  - 20200317
IS  - 2241-6293 (Electronic)
IS  - 1107-0625 (Linking)
VI  - 24
IP  - 3
DP  - 2019 May-Jun
TI  - A flavone, Wogonin from Scutellaria baicalensis inhibits the proliferation of 
      human colorectal cancer cells by inducing of autophagy, apoptosis and G2/M cell 
      cycle arrest via modulating the PI3K/AKT and STAT3 signalling pathways.
PG  - 1143-1149
AB  - PURPOSE: The purpose of this study was to examine the anticancer effects of a 
      flavone from Scutellaria baicalensis wogonin against a panel of colorectal cancer 
      cells. METHODS: The SW1417, SW48, DLD-1, HCT-15, LS-180 and CCD-18Co cell lines 
      were used for the evaluation of the anticancer effects of wogonin. WST-1 and 
      colony formation assays were used for cell viability assessment. Cell cycle 
      analysis was assessed by flow cytometry. Autophagy was detected by electron 
      microscopy. Apoptosis was detected by acridine orange (AO)/ethidium bromide (EB) 
      staining. Cell protein expression was checked by western blotting. RESULTS: The 
      cytotoxic effects of wogonin were comparatively negligible against the normal 
      CCD-18Co cells with an IC50 of >100 microM. Investigation of the mechanism of action 
      revealed that wogonin exerts growth inhibitory effects on the SW48 colorectal 
      cancer cells by autophagic and apoptotic cell death. This was also accompanied 
      with upregulation of autophagic proteins such as LC3II and Beclin 1 as well as 
      the apoptotic proteins such as caspase 3, 8 and 9 and Bax expressions. Wogonin 
      also induced arrest of the SW48 cells at the G2/M check point of the cell cycle. 
      In addition, wogonin could also inhibit the PI3K/AKT and STAT3 signal 
      transduction pathways. CONCLUSION: These results suggest that wogonin exerts 
      potent anticancer effects on colorectal cancer cells and may prove essential in 
      the management of colorectal cancer.
FAU - Tan, Hao
AU  - Tan H
AD  - Shanxi Province Academy of Traditional Chinese Medicine, Taiyuan, Shanxi 
      Province, 030012, China.
FAU - Li, Xiang
AU  - Li X
FAU - Yang, Wei Han
AU  - Yang WH
FAU - Kang, Yong
AU  - Kang Y
LA  - eng
PT  - Journal Article
PL  - Cyprus
TA  - J BUON
JT  - Journal of B.U.ON. : official journal of the Balkan Union of Oncology
JID - 100883428
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Flavanones)
RN  - 0 (STAT3 Transcription Factor)
RN  - 0 (STAT3 protein, human)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - POK93PO28W (wogonin)
SB  - IM
MH  - Apoptosis
MH  - Autophagy
MH  - Cell Proliferation
MH  - Drugs, Chinese Herbal/pharmacology/*therapeutic use
MH  - Flavanones/pharmacology/*therapeutic use
MH  - Humans
MH  - Phosphatidylinositol 3-Kinases/*metabolism
MH  - Proto-Oncogene Proteins c-akt/*metabolism
MH  - STAT3 Transcription Factor/*metabolism
MH  - Scutellaria baicalensis
MH  - Signal Transduction
EDAT- 2019/08/20 06:00
MHDA- 2020/03/18 06:00
CRDT- 2019/08/20 06:00
PHST- 2019/08/20 06:00 [entrez]
PHST- 2019/08/20 06:00 [pubmed]
PHST- 2020/03/18 06:00 [medline]
PST - ppublish
SO  - J BUON. 2019 May-Jun;24(3):1143-1149.