PMID- 31424682
OWN - NLM
STAT- MEDLINE
DCOM- 20200317
LR  - 20200317
IS  - 2241-6293 (Electronic)
IS  - 1107-0625 (Linking)
VI  - 24
IP  - 3
DP  - 2019 May-Jun
TI  - In vitro and in vivo growth inhibition of human leukemia cells by Nodakenetin are 
      mediated via mitochondrial apoptosis, cell cycle arrest and inhibition of cell 
      migration and invasion.
PG  - 1219-1224
AB  - PURPOSE: To study the anticancer potential of a plant-derived coumarin, 
      Nodakenetin, against acute lymphocytic leukemia HL-60 cells. METHODS: The 
      proliferation rate of the leukemia cells was checked by CCK-8 assay. Apoptotic 
      cell death was studied by acridine orange (AO)/ethidium bromide (EB) staining. 
      Cell cycle analysis was performed by flow cytometery. Cell migration and invasion 
      were checked by transwell assay. Protein expression were determined by immuno 
      blotting. Xenografted mice models were used for in vivo evaluation of 
      Nodakenetin. RESULTS: Nodakenetin could significantly inhibit the proliferation 
      of the all the leukemia cells. The anticancer activity of Nodakenetin against the 
      HL-60 cells was found to be due to G2/M cell cycle arrest and induction of 
      apoptosis. Nodakenetin prompted mitochondrial apoptosis which was also associated 
      with alteration in the apoptosis-related protein expression (Bax and Bcl-2). It 
      was also observed that Nodakenetin could inhibit the migration and invasion of 
      the leukemia cells in a concentration-dependent manner. The effects of the 
      Nodakenetin were also investigated in vivo in xenografted mice models and it was 
      found that this molecule could inhibit the growth of xenografted tumors. 
      CONCLUSIONS: These results indicate Nodakenetin significantly inhibits the growth 
      of leukemia cells in vitro and in vivo and may be a valuable molecule in the 
      management of leukemia, and as such needs further in depth investigations.
FAU - Lei, Ping
AU  - Lei P
AD  - Department of Hematology, Hunan Provincial People's Hospital, Changsha, 410002 
      Hunan, China.
FAU - Liao, Chushu
AU  - Liao C
FAU - Chen, Jianjun
AU  - Chen J
FAU - Zhou, Ming
AU  - Zhou M
LA  - eng
PT  - Journal Article
PL  - Cyprus
TA  - J BUON
JT  - Journal of B.U.ON. : official journal of the Balkan Union of Oncology
JID - 100883428
RN  - 0 (Coumarins)
RN  - A4VZ22K1WT (coumarin)
RN  - H5D33D6K5D (marmesin)
SB  - IM
MH  - Animals
MH  - Apoptosis/*genetics
MH  - Cell Cycle Checkpoints/*genetics
MH  - Cell Movement
MH  - Coumarins/pharmacology/*therapeutic use
MH  - Disease Models, Animal
MH  - HL-60 Cells
MH  - Humans
MH  - Leukemia/*drug therapy/pathology
MH  - Mice
MH  - Mitochondria/*metabolism
EDAT- 2019/08/20 06:00
MHDA- 2020/03/18 06:00
CRDT- 2019/08/20 06:00
PHST- 2019/08/20 06:00 [entrez]
PHST- 2019/08/20 06:00 [pubmed]
PHST- 2020/03/18 06:00 [medline]
PST - ppublish
SO  - J BUON. 2019 May-Jun;24(3):1219-1224.