PMID- 3142518
OWN - NLM
STAT- MEDLINE
DCOM- 19890109
LR  - 20190613
IS  - 0006-2960 (Print)
IS  - 0006-2960 (Linking)
VI  - 27
IP  - 16
DP  - 1988 Aug 9
TI  - Function of dopachrome oxidoreductase and metal ions in dopachrome conversion in 
      the eumelanin pathway.
PG  - 6156-9
AB  - The conversion of dopachrome (DC) in the eumelanin pathway has been analyzed to 
      determine the specific product and the role of enzyme control. 
      5,6-Dihydroxyindole (DHI) and 5,6-dihydroxyindole-2-carboxylic acid (DHICA) were 
      quantitated by HPLC with fluorescent detection, after DC incubation with heated 
      and unheated preparations of B-16 melanoma derived dopachrome oxidoreductase 
      (DCOR). The enzyme-catalyzed reaction produced DHICA as the major product, while 
      DHI formed with the spontaneous reaction. It had originally been suggested that 
      the major product of DC conversion was DHI, with DHICA being formed as a minor 
      product of this conversion [Raper, H.S. (1927) Biochem. J. 21, 89-96]. Copper, 
      nickel, and cobalt ions promoted conversion of DC, with nickel simulating DCOR 
      activity. Removal of free ions from unheated DCOR did not alter DC conversion. We 
      conclude that the major product of DC conversion is DHICA and that DCOR is 
      responsible for this conversion.
FAU - Leonard, L J
AU  - Leonard LJ
AD  - Department of Genetics and Cell Biology, University of Minnesota, Minneapolis 
      55455.
FAU - Townsend, D
AU  - Townsend D
FAU - King, R A
AU  - King RA
LA  - eng
GR  - AM32407/AM/NIADDK NIH HHS/United States
GR  - GM22167/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 0 (Indolequinones)
RN  - 0 (Indoles)
RN  - 0 (Melanins)
RN  - 0 (Metals)
RN  - 0 (Quinones)
RN  - 12627-86-0 (eumelanin)
RN  - 3571-34-4 (dopachrome)
RN  - 4790-08-3 (5,6-dihydroxy-2-indolylcarboxylic acid)
RN  - EC 1.10.3.1 (Catechol Oxidase)
RN  - EC 5.3.- (Intramolecular Oxidoreductases)
RN  - EC 5.3.3.12 (dopachrome isomerase)
RN  - Z3OC8499KG (5,6-dihydroxyindole)
SB  - IM
MH  - Animals
MH  - Catechol Oxidase/*metabolism
MH  - Hot Temperature
MH  - In Vitro Techniques
MH  - *Indolequinones
MH  - Indoles/*metabolism
MH  - *Intramolecular Oxidoreductases
MH  - Melanins/*metabolism
MH  - Melanocytes/metabolism
MH  - Melanoma, Experimental/metabolism
MH  - Metals
MH  - Mice
MH  - Quinones/*metabolism
EDAT- 1988/08/09 00:00
MHDA- 1988/08/09 00:01
CRDT- 1988/08/09 00:00
PHST- 1988/08/09 00:00 [pubmed]
PHST- 1988/08/09 00:01 [medline]
PHST- 1988/08/09 00:00 [entrez]
AID - 10.1021/bi00416a049 [doi]
PST - ppublish
SO  - Biochemistry. 1988 Aug 9;27(16):6156-9. doi: 10.1021/bi00416a049.