PMID- 31425564
OWN - NLM
STAT- MEDLINE
DCOM- 20200402
LR  - 20200402
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 14
IP  - 8
DP  - 2019
TI  - Effects of oxygen-glucose deprivation (OGD) on barrier properties and mRNA 
      transcript levels of selected marker proteins in brain endothelial 
      cells/astrocyte co-cultures.
PG  - e0221103
LID - 10.1371/journal.pone.0221103 [doi]
LID - e0221103
AB  - Ischemic stroke has been shown to induce breakdown of the blood-brain barrier, 
      although these changes are not fully characterized. Oxygen-glucose deprivation 
      (OGD) has been used to investigate the effects of ischemia in cultured brain 
      capillary endothelial cells, however this involves a change of medium which in 
      itself may affect the cells. The aim of the present study was to investigate the 
      effect of OGD and simple medium exchange followed by 48 h of reperfusion on 
      barrier properties of primary bovine endothelial cells co-cultured with rat 
      astrocytes. Barrier properties were evaluated by transendothelial electrical 
      resistance measurements, passive permeability of flux markers, RT-qPCR and 
      immunocytochemistry. Both OGD and simple medium exchange caused an increase in 
      endothelial monolayer permeability. This correlated with reduced transcript 
      levels of a number of tight junction and tight junction-associated proteins 
      (claudin-1, claudin-5, occludin, ZO-1, tricellulin, marveld3 and PECAM-1), as 
      well as with altered transcript level of several transporters and receptors 
      (GLUT-1, HB-EGF, InsR, TfR, two members of the low density lipoprotein receptor 
      family, LDLR and LRP-1, and the efflux transporter BCRP). In contrast, effects 
      induced specifically by OGD were transient de-localization of claudin-5 from the 
      junction zone, increased InsR localization at the plasma membrane and transient 
      downregulation of MRP-1 and P-gp transcript levels. In conclusion, OGD caused 
      changes in claudin-5 and InsR localization, as well as in MRP-1 and P-gp 
      transcript levels. Our results however also indicated that medium exchange alone 
      caused changes in functional barrier properties and expression levels of wide 
      range of proteins.
FAU - Tornabene, Erica
AU  - Tornabene E
AD  - Department of Pharmacy, Faculty of Health and Medical Sciences, University of 
      Copenhagen, Copenhagen, Denmark.
FAU - Helms, Hans Christian Cederberg
AU  - Helms HCC
AD  - Department of Pharmacy, Faculty of Health and Medical Sciences, University of 
      Copenhagen, Copenhagen, Denmark.
FAU - Pedersen, Stine Falsig
AU  - Pedersen SF
AD  - Department of Biology, Faculty of Science, University of Copenhagen, Copenhagen, 
      Denmark.
FAU - Brodin, Birger
AU  - Brodin B
AUID- ORCID: 0000-0002-0823-1220
AD  - Department of Pharmacy, Faculty of Health and Medical Sciences, University of 
      Copenhagen, Copenhagen, Denmark.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190819
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Biomarkers)
RN  - 0 (RNA, Messenger)
RN  - IY9XDZ35W2 (Glucose)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - Astrocytes
MH  - Biomarkers/metabolism
MH  - Blood-Brain Barrier/cytology/*metabolism/pathology
MH  - Brain Infarction/pathology
MH  - Capillaries/cytology
MH  - Cattle
MH  - Cell Hypoxia
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Endothelial Cells
MH  - Endothelium, Vascular/cytology
MH  - Gene Expression Profiling
MH  - Glucose/*metabolism
MH  - Oxygen/*metabolism
MH  - Permeability
MH  - Primary Cell Culture/methods
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Tight Junctions/pathology
PMC - PMC6699694
COIS- The authors have declared that no competing interests exist.
EDAT- 2019/08/20 06:00
MHDA- 2020/04/03 06:00
PMCR- 2019/08/19
CRDT- 2019/08/20 06:00
PHST- 2019/03/17 00:00 [received]
PHST- 2019/07/30 00:00 [accepted]
PHST- 2019/08/20 06:00 [entrez]
PHST- 2019/08/20 06:00 [pubmed]
PHST- 2020/04/03 06:00 [medline]
PHST- 2019/08/19 00:00 [pmc-release]
AID - PONE-D-19-03842 [pii]
AID - 10.1371/journal.pone.0221103 [doi]
PST - epublish
SO  - PLoS One. 2019 Aug 19;14(8):e0221103. doi: 10.1371/journal.pone.0221103. 
      eCollection 2019.