PMID- 31429513
OWN - NLM
STAT- MEDLINE
DCOM- 20200205
LR  - 20231014
IS  - 1939-1676 (Electronic)
IS  - 0891-6640 (Print)
IS  - 0891-6640 (Linking)
VI  - 33
IP  - 5
DP  - 2019 Sep
TI  - Development of a comprehensive protein microarray for immunoglobulin E profiling 
      in horses with severe asthma.
PG  - 2327-2335
LID - 10.1111/jvim.15564 [doi]
AB  - BACKGROUND: Severe asthma in horses, known as severe equine asthma (SEA), is a 
      prevalent, performance-limiting disease associated with increased 
      allergen-specific immunoglobulin E (IgE) against a range of environmental 
      aeroallergens. OBJECTIVE: To develop a protein microarray platform to profile IgE 
      against a range of proven and novel environmental proteins in SEA-affected 
      horses. ANIMALS: Six SEA-affected and 6 clinically healthy Warmblood performance 
      horses. METHODS: Developed a protein microarray (n = 384) using protein extracts 
      and purified proteins from a large number of families including pollen, bacteria, 
      fungi, and arthropods associated with the horses, environment. Conditions were 
      optimized and assessed for printing, incubation, immunolabeling, biological fluid 
      source, concentration techniques, reproducibility, and specificity. RESULTS: This 
      method identified a number of novel allergens, while also identifying an 
      association between SEA and pollen sensitization. Immunolabeling methods 
      confirmed the accuracy of a commercially available mouse anti-horse IgE 3H10 
      source (R(2) = 0.91). Biological fluid source evaluation indicated that sera and 
      bronchoalveolar lavage fluid (BALF) yielded the same specific IgE profile 
      (average R(2) = 0.75). Amicon centrifugal filters were found to be the most 
      efficient technique for concentrating BALF for IgE analysis at 40-fold. Overnight 
      incubation maintained the same sensitization profile while increasing 
      sensitivity. Reproducibility was demonstrated (R(2) = 0.97), as was specificity 
      using protein inhibition assays. Arthropods, fungi, and pollens showed the 
      greatest discrimination for SEA. CONCLUSIONS AND CLINICAL IMPORTANCE: We have 
      established that protein microarrays can be used for large-scale IgE mapping of 
      allergens associated with the environment of horses. This technology provides a 
      sound platform for specific diagnosis, management, and treatment of SEA.
CI  - (c) 2019 The Authors. Journal of Veterinary Internal Medicine published by Wiley 
      Periodicals, Inc. on behalf of the American College of Veterinary Internal 
      Medicine.
FAU - White, Samuel
AU  - White S
AUID- ORCID: 0000-0002-3675-7545
AD  - School of Equine Management and Science, Royal Agricultural University, 
      Gloucestershire, United Kingdom.
AD  - School of Biosciences, University of Nottingham, Loughborough, United Kingdom.
AD  - Animal and Equine Science, Nottingham Trent University, Nottinghamshire, United 
      Kingdom.
FAU - Moore-Colyer, Meriel
AU  - Moore-Colyer M
AD  - School of Equine Management and Science, Royal Agricultural University, 
      Gloucestershire, United Kingdom.
FAU - Marti, Eliane
AU  - Marti E
AD  - Department of Clinical Research and Veterinary Public Health, University of Bern, 
      Bern, Switzerland.
FAU - Couetil, Laurent
AU  - Couetil L
AUID- ORCID: 0000-0002-1516-6666
AD  - Veterinary Clinical Sciences, College of Veterinary Medicine, Purdue University, 
      West Lafayette, Indiana, USA.
FAU - Hannant, Duncan
AU  - Hannant D
AD  - School of Veterinary Medicine and Science, University of Nottingham, 
      Loughborough, United Kingdom.
FAU - Richard, Eric A
AU  - Richard EA
AD  - LABEO Frank Duncombe, Caen Cedex, France.
AD  - Normandie University, UniCaen, BIOTARGEN, Saint-Contest, France.
FAU - Alcocer, Marcos
AU  - Alcocer M
AD  - School of Biosciences, University of Nottingham, Loughborough, United Kingdom.
LA  - eng
GR  - Fred And Marjorie Sainsbury Charitable Trust/
GR  - HAYGAIN/
GR  - The Royal Agricultural University/
PT  - Journal Article
DEP - 20190820
PL  - United States
TA  - J Vet Intern Med
JT  - Journal of veterinary internal medicine
JID - 8708660
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
CIN - Equine Vet J. 2020 Jan;52(1):11-12. PMID: 31821611
MH  - Animals
MH  - Arthropods/immunology
MH  - Asthma/blood/immunology/*veterinary
MH  - Bronchoalveolar Lavage Fluid/*immunology
MH  - Case-Control Studies
MH  - Fungi/immunology
MH  - Horse Diseases/blood/*immunology
MH  - Horses
MH  - Immunoglobulin E/*blood/immunology
MH  - Mice
MH  - Pollen/immunology
MH  - Protein Array Analysis/methods/*veterinary
PMC - PMC6766494
OTO - NOTNLM
OT  - IgE
OT  - allergen
OT  - horse
OT  - protein microarray
OT  - severe equine asthma
COIS- Authors declare no conflict of interest.
EDAT- 2019/08/21 06:00
MHDA- 2020/02/06 06:00
PMCR- 2019/09/01
CRDT- 2019/08/21 06:00
PHST- 2019/01/05 00:00 [received]
PHST- 2019/07/01 00:00 [accepted]
PHST- 2019/08/21 06:00 [pubmed]
PHST- 2020/02/06 06:00 [medline]
PHST- 2019/08/21 06:00 [entrez]
PHST- 2019/09/01 00:00 [pmc-release]
AID - JVIM15564 [pii]
AID - 10.1111/jvim.15564 [doi]
PST - ppublish
SO  - J Vet Intern Med. 2019 Sep;33(5):2327-2335. doi: 10.1111/jvim.15564. Epub 2019 
      Aug 20.