PMID- 31430717
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 2162-2531 (Print)
IS  - 2162-2531 (Electronic)
IS  - 2162-2531 (Linking)
VI  - 17
DP  - 2019 Sep 6
TI  - lncRNA NR_038323 Suppresses Renal Fibrosis in Diabetic Nephropathy by Targeting 
      the miR-324-3p/DUSP1 Axis.
PG  - 741-753
LID - S2162-2531(19)30197-0 [pii]
LID - 10.1016/j.omtn.2019.07.007 [doi]
AB  - Several studies have suggested that long intergenic noncoding RNAs are involved 
      in the progression of diabetic nephropathy (DN). However, the exact role and 
      regulatory mechanism of long noncoding RNA (lncRNA) NR_038323 in diabetic 
      nephropathy (DN) remain largely unclear. In the present study, we found that 
      lncRNA NR_038323 overexpression ameliorated the high glucose (HG)-induced 
      expression levels of collagen I, collagen IV, and fibronectin, whereas lncRNA 
      NR_038323 knockdown exerted the opposite effects. Moreover, the results of 
      bioinformatic prediction, luciferase assay, and fluorescence in situ 
      hybridization (FISH) demonstrated that lncRNA NR_038323 directly interacted with 
      miR-324-3p. Additionally, miR-324-3p mimic aggravated the HG-induced expression 
      levels of collagen I, collagen IV, and fibronectin by dual-specificity protein 
      phosphatase-1 (DUSP1) expression to activate p38 mitogen-activated protein kinase 
      (MAPK) and ERK1/2 pathways. In contrast, overexpression of DUSP1 attenuated the 
      HG-induced expression levels of collagen I, collagen IV, and fibronectin via 
      inactivation of p38 MAPK and ERK1/2 pathways. In addition, lncRNA NR_038323 
      knockdown increased the expression levels of collagen I, collagen IV, and 
      fibronectin by upregulating DUSP1 expression during HG treatment, which were 
      markedly reversed by miR-324-3p inhibitor. Furthermore, these molecular changes 
      were verified in the human kidney samples of DN patients. Finally, overexpression 
      of lncRNA NR_038323 ameliorated the interstitial fibrosis in STZ-induced diabetic 
      nephrology (DN) rat via miR-324-3p/DUSP1/p38MAPK and ERK1/2 axis. In conclusion, 
      our data indicate that overexpression of lncRNA NR_038323 may suppress HG-induced 
      renal fibrosis via the miR-324-3p/DUSP1/p38MAPK and ERK1/2 axis, which provides 
      new insights into the pathogenesis of DN.
CI  - Copyright (c) 2019 The Author(s). Published by Elsevier Inc. All rights reserved.
FAU - Ge, Yanni
AU  - Ge Y
AD  - Department of Emergency Medicine, Second Xiangya Hospital, Central South 
      University, Changsha, Hunan, People's Republic of China; Department of 
      Ophthalmology, Second Xiangya Hospital, Central South University, Changsha, 
      Hunan, People's Republic of China.
FAU - Wang, Juan
AU  - Wang J
AD  - Department of Emergency Medicine, Second Xiangya Hospital, Central South 
      University, Changsha, Hunan, People's Republic of China; Department of 
      Ophthalmology, Second Xiangya Hospital, Central South University, Changsha, 
      Hunan, People's Republic of China.
FAU - Wu, Dengke
AU  - Wu D
AD  - Department of Emergency Medicine, Second Xiangya Hospital, Central South 
      University, Changsha, Hunan, People's Republic of China.
FAU - Zhou, Yu
AU  - Zhou Y
AD  - Department of Emergency Medicine, Second Xiangya Hospital, Central South 
      University, Changsha, Hunan, People's Republic of China.
FAU - Qiu, Shuangfa
AU  - Qiu S
AD  - Department of Emergency Medicine, Second Xiangya Hospital, Central South 
      University, Changsha, Hunan, People's Republic of China.
FAU - Chen, Junxiang
AU  - Chen J
AD  - Department of Ophthalmology, Second Xiangya Hospital, Central South University, 
      Changsha, Hunan, People's Republic of China.
FAU - Zhu, Xuejin
AU  - Zhu X
AD  - Department of Nephrology, Second Xiangya Hospital, Central South University, 
      Changsha, Hunan, People's Republic of China.
FAU - Xiang, Xudong
AU  - Xiang X
AD  - Department of Emergency Medicine, Second Xiangya Hospital, Central South 
      University, Changsha, Hunan, People's Republic of China.
FAU - Li, Huiling
AU  - Li H
AD  - Department of Emergency Medicine, Second Xiangya Hospital, Central South 
      University, Changsha, Hunan, People's Republic of China; Department of 
      Ophthalmology, Second Xiangya Hospital, Central South University, Changsha, 
      Hunan, People's Republic of China. Electronic address: lihuiling1120@163.com.
FAU - Zhang, Dongshan
AU  - Zhang D
AD  - Department of Emergency Medicine, Second Xiangya Hospital, Central South 
      University, Changsha, Hunan, People's Republic of China; Department of 
      Nephrology, Second Xiangya Hospital, Central South University, Changsha, Hunan, 
      People's Republic of China. Electronic address: dongshanzhang@csu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20190723
PL  - United States
TA  - Mol Ther Nucleic Acids
JT  - Molecular therapy. Nucleic acids
JID - 101581621
PMC - PMC6709345
OTO - NOTNLM
OT  - DUSP1
OT  - diabetic nephropathy
OT  - lncRNA NR_038323
OT  - miR-324-3p
EDAT- 2019/08/21 06:00
MHDA- 2019/08/21 06:01
PMCR- 2019/07/23
CRDT- 2019/08/21 06:00
PHST- 2019/02/04 00:00 [received]
PHST- 2019/06/21 00:00 [revised]
PHST- 2019/07/10 00:00 [accepted]
PHST- 2019/08/21 06:00 [pubmed]
PHST- 2019/08/21 06:01 [medline]
PHST- 2019/08/21 06:00 [entrez]
PHST- 2019/07/23 00:00 [pmc-release]
AID - S2162-2531(19)30197-0 [pii]
AID - 10.1016/j.omtn.2019.07.007 [doi]
PST - ppublish
SO  - Mol Ther Nucleic Acids. 2019 Sep 6;17:741-753. doi: 10.1016/j.omtn.2019.07.007. 
      Epub 2019 Jul 23.