PMID- 31432086
OWN - NLM
STAT- MEDLINE
DCOM- 20200821
LR  - 20200821
IS  - 1096-0929 (Electronic)
IS  - 1096-0929 (Linking)
VI  - 172
IP  - 2
DP  - 2019 Dec 1
TI  - A Human Relevant Defined Mixture of Persistent Organic Pollutants (POPs) Affects 
      In Vitro Secretion of Glucagon-Like Peptide 1 (GLP-1), but Does Not Affect 
      Translocation of Its Receptor.
PG  - 359-367
LID - 10.1093/toxsci/kfz192 [doi]
AB  - Environmental exposure to persistent organic pollutants (POPs) has been suggested 
      as a contributing factor for the increased rate of type 2 diabetes and obesity. A 
      complex mixture of 29 POPs (Total mixture), based on human blood concentrations, 
      was used to expose a glucagon-like peptide 1 (GLP-1) secreting enteroendocrine 
      cell line (pGIP/neo: STC-1) in vitro for 3 and 24 h. Significant increases of 
      GLP-1 occurred when cells were exposed to the Total mixture at x500 blood levels. 
      Six sub-mixtures representing chlorinated (Cl), brominated (Br), and 
      perfluorinated chemicals (PFAA), and their combinations (Cl + Br, Cl + PFAA, Br + 
      PFAA) were also tested at x500. Secretion levels seen for these remained lower 
      than the Total mixture, and the Br mixture had no effect. After 24 h, increased 
      secretion was seen with all mixtures at x1 blood levels. Cytotoxicity was present 
      for x100 and x500 blood levels. When tested in a GLP-1 receptor translocation 
      assay (U2OS-GLP1R-EGFP), neither agonistic nor antagonist effects on receptor 
      internalization were seen for any of the mixtures. We conclude individual classes 
      of POPs, alone or in combination, can affect GLP-1 secretion and may contribute 
      as a molecular mechanism linking environmental toxicants and diabetes.
CI  - (c) The Author(s) 2019. Published by Oxford University Press on behalf of the 
      Society of Toxicology. All rights reserved. For permissions, please e-mail: 
      journals.permissions@oup.com.
FAU - Shannon, Maeve
AU  - Shannon M
AD  - Institute for Global Food Security, School of Biological Sciences, Queen's 
      University Belfast, Belfast, BT9 5DL, Northern Ireland, UK.
FAU - Xie, Yuling
AU  - Xie Y
AD  - Institute for Global Food Security, School of Biological Sciences, Queen's 
      University Belfast, Belfast, BT9 5DL, Northern Ireland, UK.
FAU - Verhaegen, Steven
AU  - Verhaegen S
AD  - Department of Production Animal Clinical Sciences, Faculty of Veterinary 
      Medicine, Norwegian University of Life Sciences, Oslo 0102, Norway.
FAU - Wilson, Jodie
AU  - Wilson J
AD  - Institute for Global Food Security, School of Biological Sciences, Queen's 
      University Belfast, Belfast, BT9 5DL, Northern Ireland, UK.
FAU - Berntsen, Hanne F
AU  - Berntsen HF
AD  - Department of Production Animal Clinical Sciences, Faculty of Veterinary 
      Medicine, Norwegian University of Life Sciences, Oslo 0102, Norway.
AD  - Department of Administration, Lab Animal Unit, National Institute of Occupational 
      Health, Oslo 0363, Norway.
FAU - Zimmer, Karin E
AU  - Zimmer KE
AD  - Department of Basic Sciences and Aquatic Medicine, Faculty of Veterinary 
      Medicine, Norwegian University of Life Sciences, Oslo 0102, Norway.
FAU - Ropstad, Erik
AU  - Ropstad E
AD  - Department of Production Animal Clinical Sciences, Faculty of Veterinary 
      Medicine, Norwegian University of Life Sciences, Oslo 0102, Norway.
FAU - Green, Brian D
AU  - Green BD
AD  - Institute for Global Food Security, School of Biological Sciences, Queen's 
      University Belfast, Belfast, BT9 5DL, Northern Ireland, UK.
FAU - Connolly, Lisa
AU  - Connolly L
AD  - Institute for Global Food Security, School of Biological Sciences, Queen's 
      University Belfast, Belfast, BT9 5DL, Northern Ireland, UK.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Toxicol Sci
JT  - Toxicological sciences : an official journal of the Society of Toxicology
JID - 9805461
RN  - 0 (Endocrine Disruptors)
RN  - 0 (Environmental Pollutants)
RN  - 0 (GLP1R protein, human)
RN  - 0 (Glucagon-Like Peptide-1 Receptor)
RN  - 0 (Hydrocarbons, Halogenated)
RN  - 89750-14-1 (Glucagon-Like Peptide 1)
SB  - IM
MH  - Cell Culture Techniques
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Endocrine Disruptors/chemistry/*toxicity
MH  - Enteroendocrine Cells/*drug effects/metabolism
MH  - Environmental Pollutants/chemistry/*toxicity
MH  - Glucagon-Like Peptide 1/*metabolism
MH  - Glucagon-Like Peptide-1 Receptor/*metabolism
MH  - Humans
MH  - Hydrocarbons, Halogenated/chemistry/*toxicity
MH  - Protein Transport
OTO - NOTNLM
OT  - ELISA
OT  - enteroendocrine cell
OT  - gut hormones
OT  - high content analysis
OT  - metabolic disruption
OT  - mixture
EDAT- 2019/08/23 06:00
MHDA- 2020/08/22 06:00
CRDT- 2019/08/22 06:00
PHST- 2019/08/23 06:00 [pubmed]
PHST- 2020/08/22 06:00 [medline]
PHST- 2019/08/22 06:00 [entrez]
AID - 5552164 [pii]
AID - 10.1093/toxsci/kfz192 [doi]
PST - ppublish
SO  - Toxicol Sci. 2019 Dec 1;172(2):359-367. doi: 10.1093/toxsci/kfz192.