PMID- 31434941
OWN - NLM
STAT- MEDLINE
DCOM- 20201022
LR  - 20210110
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 9
IP  - 1
DP  - 2019 Aug 21
TI  - High-speed device synchronization in optical microscopy with an open-source 
      hardware control platform.
PG  - 12188
LID - 10.1038/s41598-019-48455-z [doi]
LID - 12188
AB  - Azimuthal beam scanning eliminates the uneven excitation field arising from laser 
      interference in through-objective total internal reflection fluorescence (TIRF) 
      microscopy. The same principle can be applied to scanning angle interference 
      microscopy (SAIM), where precision control of the scanned laser beam presents 
      unique technical challenges for the builders of custom azimuthal scanning 
      microscopes. Accurate synchronization between the instrument computer, beam 
      scanning system and excitation source is required to collect high quality data 
      and minimize sample damage in SAIM acquisitions. Drawing inspiration from 
      open-source prototyping systems, like the Arduino microcontroller boards, we 
      developed a new instrument control platform to be affordable, easily programmed, 
      and broadly useful, but with integrated, precision analog circuitry and optimized 
      firmware routines tailored to advanced microscopy. We show how the integration of 
      waveform generation, multiplexed analog outputs, and native hardware triggers 
      into a single central hub provides a versatile platform for performing fast 
      circle-scanning acquisitions, including azimuthal scanning SAIM and multiangle 
      TIRF. We also demonstrate how the low communication latency of our hardware 
      platform can reduce image intensity and reconstruction artifacts arising from 
      synchronization errors produced by software control. Our complete platform, 
      including hardware design, firmware, API, and software, is available online for 
      community-based development and collaboration.
FAU - Colville, Marshall J
AU  - Colville MJ
AUID- ORCID: 0000-0002-5397-8382
AD  - Field of Biophysics, Cornell University, Ithaca, NY, 14853, USA. 
      mjc449@cornell.edu.
FAU - Park, Sangwoo
AU  - Park S
AUID- ORCID: 0000-0002-2701-3114
AD  - Field of Biophysics, Cornell University, Ithaca, NY, 14853, USA.
FAU - Zipfel, Warren R
AU  - Zipfel WR
AD  - Field of Biophysics, Cornell University, Ithaca, NY, 14853, USA.
AD  - Nancy E. and Peter C. Meinig School of Biomedical Engineering, Cornell 
      University, Ithaca, NY, 14853, USA.
AD  - Kavli Institute at Cornell for Nanoscale Science, Ithaca, NY, 14853, USA.
FAU - Paszek, Matthew J
AU  - Paszek MJ
AD  - Field of Biophysics, Cornell University, Ithaca, NY, 14853, USA. 
      mjp31@cornell.edu.
AD  - Robert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell 
      University, Ithaca, NY, 14853, USA. mjp31@cornell.edu.
AD  - Field of Biomedical Engineering, Cornell University, Ithaca, NY, 14853, USA. 
      mjp31@cornell.edu.
AD  - Kavli Institute at Cornell for Nanoscale Science, Ithaca, NY, 14853, USA. 
      mjp31@cornell.edu.
LA  - eng
GR  - T32 GM008267/GM/NIGMS NIH HHS/United States
GR  - U54 CA210184/CA/NCI NIH HHS/United States
GR  - R33-CA193043/U.S. Department of Health & Human Services | NIH | National 
      Cancer Institute (NCI)/International
GR  - U54-CS210184/U.S. Department of Health & Human Services | NIH | National 
      Cancer Institute (NCI)/International
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20190821
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Lipid Bilayers)
SB  - IM
MH  - Artifacts
MH  - Equipment Design
MH  - HeLa Cells
MH  - Humans
MH  - Lasers
MH  - Lipid Bilayers/chemistry
MH  - Microscopy, Fluorescence/instrumentation/*methods
MH  - Microscopy, Interference/instrumentation/methods
MH  - Software
PMC - PMC6704125
COIS- The authors declare no competing interests.
EDAT- 2019/08/23 06:00
MHDA- 2020/10/23 06:00
PMCR- 2019/08/21
CRDT- 2019/08/23 06:00
PHST- 2019/02/20 00:00 [received]
PHST- 2019/07/31 00:00 [accepted]
PHST- 2019/08/23 06:00 [entrez]
PHST- 2019/08/23 06:00 [pubmed]
PHST- 2020/10/23 06:00 [medline]
PHST- 2019/08/21 00:00 [pmc-release]
AID - 10.1038/s41598-019-48455-z [pii]
AID - 48455 [pii]
AID - 10.1038/s41598-019-48455-z [doi]
PST - epublish
SO  - Sci Rep. 2019 Aug 21;9(1):12188. doi: 10.1038/s41598-019-48455-z.