PMID- 31435691
OWN - NLM
STAT- MEDLINE
DCOM- 20200915
LR  - 20200915
IS  - 1432-184X (Electronic)
IS  - 0095-3628 (Linking)
VI  - 79
IP  - 3
DP  - 2020 Apr
TI  - Early Life Stages of a Common Broadcast Spawning Coral Associate with Specific 
      Bacterial Communities Despite Lack of Internalized Bacteria.
PG  - 706-719
LID - 10.1007/s00248-019-01428-1 [doi]
AB  - Coral-associated bacteria are critical for the well-being of their host and may 
      play essential roles during ontogeny, as suggested by the vertical transmission 
      of some bacteria in brooding corals. Bacterial acquisition patterns in broadcast 
      spawners remain uncertain, as 16S rRNA gene metabarcoding of coral early life 
      stages suggests the presence of bacterial communities, which have not been 
      detected by microscopic examinations. Here, we combined 16S rRNA gene 
      metabarcoding with fluorescence in situ hybridization (FISH) microscopy to 
      analyze bacterial assemblages in Acropora tenuis egg-sperm bundles, embryos, and 
      larvae following a spawning event. Metabarcoding results indicated that A. tenuis 
      offspring </= 4-day-old were associated with diverse and dynamic bacterial 
      microbiomes, dominated by Rhodobacteraceae, Alteromonadaceae, and 
      Oceanospirillaceae. While FISH analyses confirmed the lack of internalized 
      bacteria in A. tenuis offspring, metabarcoding showed that even the earliest life 
      stages examined (egg-sperm bundles and two-cell stages) were associated with a 
      diverse bacterial community, suggesting the bacteria were confined to the mucus 
      layer. These results can be explained by vertical transmission of certain taxa 
      (mainly Endozoicomonas) in the mucus surrounding the gametes within bundles, or 
      by horizontal bacterial transmission through the release of bacteria by spawning 
      adults into the water column.
FAU - Damjanovic, Katarina
AU  - Damjanovic K
AUID- ORCID: 0000-0002-3893-4381
AD  - School of BioSciences, The University of Melbourne, Parkville, VIC, 3010, 
      Australia. kdamjanovic@student.unimelb.edu.au.
AD  - Australian Institute of Marine Science, PMB No 3, Townsville MC, QLD, 4810, 
      Australia. kdamjanovic@student.unimelb.edu.au.
FAU - Menendez, Patricia
AU  - Menendez P
AD  - Australian Institute of Marine Science, PMB No 3, Townsville MC, QLD, 4810, 
      Australia.
AD  - Department of Econometrics and Business Statistics, Monash University, Clayton, 
      VIC, 3800, Australia.
FAU - Blackall, Linda L
AU  - Blackall LL
AD  - School of BioSciences, The University of Melbourne, Parkville, VIC, 3010, 
      Australia.
FAU - van Oppen, Madeleine J H
AU  - van Oppen MJH
AD  - School of BioSciences, The University of Melbourne, Parkville, VIC, 3010, 
      Australia.
AD  - Australian Institute of Marine Science, PMB No 3, Townsville MC, QLD, 4810, 
      Australia.
LA  - eng
GR  - DP160101468/Australian Research Council/
GR  - Holsworth Wildlife Endowment/Ecological Society of Australia/
PT  - Journal Article
DEP - 20190821
PL  - United States
TA  - Microb Ecol
JT  - Microbial ecology
JID - 7500663
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - Anthozoa/growth & development/*microbiology
MH  - *Bacterial Physiological Phenomena
MH  - Embryo, Nonmammalian/microbiology
MH  - In Situ Hybridization, Fluorescence
MH  - Larva/microbiology
MH  - *Life Cycle Stages
MH  - Male
MH  - Microbiota/*physiology
MH  - Ovum/microbiology
MH  - RNA, Bacterial/analysis
MH  - RNA, Ribosomal, 16S/analysis
MH  - Reproduction
MH  - Spermatozoa/microbiology
OTO - NOTNLM
OT  - Acropora tenuis
OT  - Bacterial communities
OT  - Broadcast spawning coral
OT  - Early life stages
OT  - Horizontal acquisition
OT  - Vertical transmission
EDAT- 2019/08/23 06:00
MHDA- 2020/09/17 06:00
CRDT- 2019/08/23 06:00
PHST- 2019/05/17 00:00 [received]
PHST- 2019/08/13 00:00 [accepted]
PHST- 2019/08/23 06:00 [pubmed]
PHST- 2020/09/17 06:00 [medline]
PHST- 2019/08/23 06:00 [entrez]
AID - 10.1007/s00248-019-01428-1 [pii]
AID - 10.1007/s00248-019-01428-1 [doi]
PST - ppublish
SO  - Microb Ecol. 2020 Apr;79(3):706-719. doi: 10.1007/s00248-019-01428-1. Epub 2019 
      Aug 21.