PMID- 31437674
OWN - NLM
STAT- MEDLINE
DCOM- 20200204
LR  - 20200204
IS  - 1878-1667 (Electronic)
IS  - 0147-9571 (Linking)
VI  - 66
DP  - 2019 Oct
TI  - Analysis of Staphylococcus infections in a veterinary teaching hospital from 2012 
      to 2015.
PG  - 101332
LID - S0147-9571(19)30129-8 [pii]
LID - 10.1016/j.cimid.2019.101332 [doi]
AB  - Records of all Diagnostic laboratory submissions from 2012 to 2015 were examined 
      and subjected to analysis according to species, location of infection, species of 
      bacteria, and antibiotic resistance/susceptibility. A total of 23.8% of all 
      culture isolates were Staphylococcus sp. Of those Staphylococcus, 43% were 
      isolated from surgical site infections. Staphylococcus pseudintermedius accounted 
      for approximately 28% of all staphylococcus cultures, while methicillin-resistant 
      (MR) S. pseudintermedius accounted for 8% of all staphylococcus cultures. 
      Environmental samples were also collected by swabbing surfaces in the intensive 
      care unit (ICU) and anesthesia prep room at the OSU VTH. Isolated bacterial 
      colonies were subjected to PCR for species identification and for the presence of 
      the mecA gene. Ability of horizontal transfer in vitro of the mecA gene was 
      evaluated by incubating the mecA positive bacterium, with the mecA negative 
      bacterium, and then plated onto agar plates infused with known concentration of 
      oxacillin. Colonies were then subjected to PCR for species and mecA 
      identification. Horizontal transfer of the mecA gene was demonstrated and 
      confirmed via PCR from MR S. epidermidis to MS S. pseudintermedius in an in vitro 
      model that mimicked the veterinary hospital environment. Biofilms were 
      established using four Staphylococcus species isolated from swabbing the 
      Intensive Care Unit (ICU) and anesthesia prep room and were resistant when 
      exposed to the current cleaning agent. Staphylococcus species makeup nearly (1/4) of 
      all infections at OSU VDL during the four years of the study, and MS S. 
      pseudintermedius was shown to acquire the mecA gene from an environmental strain.
CI  - Copyright (c) 2019 The Authors. Published by Elsevier Ltd.. All rights reserved.
FAU - Shoen, Hanna R C
AU  - Shoen HRC
AD  - Department of Biomedical Sciences, Carlson College of Veterinary Medicine, Oregon 
      State University, Corvallis, OR, United States.
FAU - Rose, Sasha J
AU  - Rose SJ
AD  - Department of Biomedical Sciences, Carlson College of Veterinary Medicine, Oregon 
      State University, Corvallis, OR, United States.
FAU - Ramsey, Stephen A
AU  - Ramsey SA
AD  - Department of Biomedical Sciences, Carlson College of Veterinary Medicine, Oregon 
      State University, Corvallis, OR, United States; School of Electrical Engineering 
      and Computer Science, College of Engineering, United States.
FAU - de Morais, Helio
AU  - de Morais H
AD  - Department of Clinical Sciences, Carlson College of Veterinary Medicine, United 
      States.
FAU - Bermudez, Luiz E
AU  - Bermudez LE
AD  - Department of Biomedical Sciences, Carlson College of Veterinary Medicine, Oregon 
      State University, Corvallis, OR, United States; Department of Microbiology, 
      College of Science, Oregon State University, Corvallis, OR, United States. 
      Electronic address: luiz.bermudez@oregonstate.edu.
LA  - eng
PT  - Journal Article
DEP - 20190716
PL  - England
TA  - Comp Immunol Microbiol Infect Dis
JT  - Comparative immunology, microbiology and infectious diseases
JID - 7808924
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Penicillin-Binding Proteins)
RN  - 0 (mecA protein, Staphylococcus aureus)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/pharmacology
MH  - Bacterial Proteins/genetics
MH  - Disinfection/methods
MH  - Drug Resistance, Bacterial/*genetics
MH  - *Hospitals, Animal
MH  - *Hospitals, Teaching
MH  - Microbial Sensitivity Tests
MH  - Penicillin-Binding Proteins/genetics
MH  - Retrospective Studies
MH  - Staphylococcal Infections/epidemiology/*veterinary
MH  - Staphylococcus/*drug effects/genetics/*isolation & purification
OTO - NOTNLM
OT  - Antibiotic
OT  - Disinfection
OT  - Hospital infection
OT  - Methicillin resistant
OT  - S. pseudintermedius
OT  - Transfer of resistance gene
EDAT- 2019/08/23 06:00
MHDA- 2020/02/06 06:00
CRDT- 2019/08/23 06:00
PHST- 2018/12/27 00:00 [received]
PHST- 2019/06/27 00:00 [revised]
PHST- 2019/07/10 00:00 [accepted]
PHST- 2019/08/23 06:00 [pubmed]
PHST- 2020/02/06 06:00 [medline]
PHST- 2019/08/23 06:00 [entrez]
AID - S0147-9571(19)30129-8 [pii]
AID - 10.1016/j.cimid.2019.101332 [doi]
PST - ppublish
SO  - Comp Immunol Microbiol Infect Dis. 2019 Oct;66:101332. doi: 
      10.1016/j.cimid.2019.101332. Epub 2019 Jul 16.