PMID- 31440325 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20231013 IS - 1939-4551 (Print) IS - 1939-4551 (Electronic) IS - 1939-4551 (Linking) VI - 12 IP - 8 DP - 2019 Aug TI - Western oropharyngeal and gut microbial profiles are associated with allergic conditions in Chinese immigrant children. PG - 100051 LID - 10.1016/j.waojou.2019.100051 [doi] LID - 100051 AB - BACKGROUND: The allergy epidemic resulting from western environment/lifestyles is potentially due to modifications of the human microbiome. Therefore, it is of interest to study immigrants living in a western environment as well as their counterparts in the country of origin to understand differences in their microbiomes and health status. METHODS: We investigated 58 Australian Chinese (AC) children from Perth, Western Australia as well as 63 Chinese-born Chinese (CC) children from a city in China. Oropharyngeal (OP) and fecal samples were collected. To assess the microbiomes, 16s ribosomal RNA (rRNA) sequencing for variable regions V3 and V4 was used. Skin prick tests (SPT) were performed to measure the children's atopic status. Information on food allergy and wheezing were acquired from a questionnaire. RESULTS: AC children had more allergic conditions than CC children. The alpha diversity (mean species diversity) of both OP and gut microbiome was lower in AC children compared to CC children for richness estimate (Chao1), while diversity evenness (Shannon index) was higher. The beta diversity (community similarity) displayed a distinct separation of the OP and gut microbiota between AC and CC children. An apparent difference in microbial abundance was observed for many bacteria. In AC children, we sought to establish consistent trends in bacterial relative abundance that are either higher or lower in AC versus CC children and higher or lower in children with allergy versus those without allergy. The majority of OP taxa showed a consistent trend while the majority of fecal taxa showed a contrasting trend. CONCLUSION: Distinct differences in microbiome compositions were found in both oropharyngeal and fecal samples of AC and CC children. The association of the OP microbiome with allergic condition is different from that of the gut microbiome in AC children. The microbiome profiles are changed by the western environment/lifestyle and are associated with allergies in Chinese immigrant children in Australia. FAU - Guo, Jing AU - Guo J AD - School of Public Health, Curtin University of Technology, Perth, WA, Australia. AD - Centre for Genetic Origins of Health and Disease, Faculty of Health Sciences, Curtin University of Technology, Faculty of Health and Medical Sciences, The University of Western Australia, Royal Perth Hospital Medical Research Foundation, Perth, WA, Australia. FAU - Lv, Quanjun AU - Lv Q AD - Department of Nutrition and Food Hygiene, College of Public Health, Zhengzhou University, Zhengzhou, Henan, China. FAU - Ariff, Amir AU - Ariff A AD - Centre for Genetic Origins of Health and Disease, Faculty of Health Sciences, Curtin University of Technology, Faculty of Health and Medical Sciences, The University of Western Australia, Royal Perth Hospital Medical Research Foundation, Perth, WA, Australia. FAU - Zhang, Xiaoping AU - Zhang X AD - China National Bamboo Research Centre, Key Laboratory of Resources and Utilization of Bamboo of State Forestry Administration, Hangzhou, Zhejiang, China. FAU - Peacock, Christopher S AU - Peacock CS AD - Marshall Centre for Infectious Disease, School of Pathology and Laboratory Medicine, The University of Western Australia, Perth, WA, Australia. AD - Telethon Kids Institute, The University of Western Australia, Perth, WA, Australia. FAU - Song, Yong AU - Song Y AD - School of Public Health, Curtin University of Technology, Perth, WA, Australia. AD - Centre for Genetic Origins of Health and Disease, Faculty of Health Sciences, Curtin University of Technology, Faculty of Health and Medical Sciences, The University of Western Australia, Royal Perth Hospital Medical Research Foundation, Perth, WA, Australia. FAU - Wen, Xiajie AU - Wen X AD - Department of Nutrition and Food Hygiene, College of Public Health, Zhengzhou University, Zhengzhou, Henan, China. FAU - Saiganesh, Aarti AU - Saiganesh A AD - Telethon Kids Institute, The University of Western Australia, Perth, WA, Australia. AD - Division of Cardiovascular and Respiratory Sciences, The University of Western Australia, Perth, WA, Australia. FAU - Melton, Phillip E AU - Melton PE AD - Centre for Genetic Origins of Health and Disease, Faculty of Health Sciences, Curtin University of Technology, Faculty of Health and Medical Sciences, The University of Western Australia, Royal Perth Hospital Medical Research Foundation, Perth, WA, Australia. AD - School of Pharmacy and Biomedical Sciences, Faculty of Health Sciences, Curtin University of Technology, Perth, WA, Australia. FAU - Dykes, Gary A AU - Dykes GA AD - School of Public Health, Curtin University of Technology, Perth, WA, Australia. FAU - Moses, Eric K AU - Moses EK AD - Centre for Genetic Origins of Health and Disease, Faculty of Health Sciences, Curtin University of Technology, Faculty of Health and Medical Sciences, The University of Western Australia, Royal Perth Hospital Medical Research Foundation, Perth, WA, Australia. AD - School of Pharmacy and Biomedical Sciences, Faculty of Health Sciences, Curtin University of Technology, Perth, WA, Australia. FAU - LE Souef, Peter N AU - LE Souef PN AD - Division of Cardiovascular and Respiratory Sciences, The University of Western Australia, Perth, WA, Australia. FAU - Lu, Fengmin AU - Lu F AD - Department of Microbiology & Infectious Disease Centre, School of Basic Medical Sciences, Peking University Health Science Centre, Beijing, China. FAU - Zhang, Guicheng AU - Zhang G AD - School of Public Health, Curtin University of Technology, Perth, WA, Australia. AD - Centre for Genetic Origins of Health and Disease, Faculty of Health Sciences, Curtin University of Technology, Faculty of Health and Medical Sciences, The University of Western Australia, Royal Perth Hospital Medical Research Foundation, Perth, WA, Australia. AD - Telethon Kids Institute, The University of Western Australia, Perth, WA, Australia. AD - Division of Cardiovascular and Respiratory Sciences, The University of Western Australia, Perth, WA, Australia. LA - eng PT - Journal Article DEP - 20190809 PL - United States TA - World Allergy Organ J JT - The World Allergy Organization journal JID - 101481283 PMC - PMC6699559 OTO - NOTNLM OT - AC, Australian Chinese OT - Allergy OT - Atopy OT - BMI, body mass index OT - CC, China-Born Chinese OT - FDR, false discovery rate OT - Immigration OT - KEGG, Kyoto Encyclopaedia of Genes and Genomes OT - LDA, The linear discriminant analysis OT - LEfSe, The linear discriminant analysis effect size OT - Microbiome OT - OP, oropharyngeal OT - PICRUSt, Phylogenetic Investigation of Communities by Reconstruction of Unobserved States OT - SPT, skin prick test OT - Western environment OT - rRNA, ribosomal RNA EDAT- 2019/08/24 06:00 MHDA- 2019/08/24 06:01 PMCR- 2019/08/09 CRDT- 2019/08/24 06:00 PHST- 2019/04/01 00:00 [received] PHST- 2019/07/09 00:00 [revised] PHST- 2019/07/19 00:00 [accepted] PHST- 2019/08/24 06:00 [entrez] PHST- 2019/08/24 06:00 [pubmed] PHST- 2019/08/24 06:01 [medline] PHST- 2019/08/09 00:00 [pmc-release] AID - S1939-4551(19)31205-0 [pii] AID - 100051 [pii] AID - 10.1016/j.waojou.2019.100051 [doi] PST - epublish SO - World Allergy Organ J. 2019 Aug 9;12(8):100051. doi: 10.1016/j.waojou.2019.100051. eCollection 2019 Aug.