PMID- 31440353
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200929
IS  - 1993-0771 (Print)
IS  - 1993-078X (Electronic)
IS  - 1993-0771 (Linking)
VI  - 13
IP  - 3
DP  - 2019
TI  - Development of new cytogenetic markers for Thinopyrum ponticum (Podp.) Z.-W. Liu 
      & R.-C. Wang.
PG  - 231-243
LID - 10.3897/CompCytogen.v13i3.36112 [doi]
AB  - Thinopyrum ponticum (Podpera, 1902) Z.-W. Liu & R.-C.Wang, 1993 is an important 
      polyploid wild perennial Triticeae species that is widely used as a source of 
      valuable genes for wheat but its genomic constitution has long been debated. For 
      its chromosome identification, only a limited set of FISH probes has been used. 
      The development of new cytogenetic markers for Th. ponticum chromosomes is of 
      great importance both for cytogenetic characterization of wheat-wheatgrass 
      hybrids and for fundamental comparative studies of phylogenetic relationships 
      between species. Here, we report on the development of five cytogenetic markers 
      for Th. ponticum based on repetitive satellite DNA of which sequences were 
      selected from the whole genome sequence of Aegilops tauschii Cosson, 1849. Using 
      real-time quantitative PCR we estimated the abundance of the found repeats: P720 
      and P427 had the highest abundance and P132, P332 and P170 had lower quantity in 
      Th. ponticum genome. Using fluorescence in situ hybridization (FISH) we localized 
      five repeats to different regions of the chromosomes of Th. ponticum. Using 
      reprobing multicolor FISH we colocalized the probes between each other. The 
      distribution of these found repeats in the Triticeae genomes and its usability as 
      cytogenetic markers for chromosomes of Th. ponticum are discussed.
FAU - Kroupin, Pavel Yu
AU  - Kroupin PY
AUID- ORCID: 0000-0001-6858-3941
AD  - Laboratory of Applied Genomics and Crop Breeding, All-Russia Research Institute 
      of Agricultural Biotechnology, Timiryazevskaya str. 42, Moscow 127550, Russia 
      All-Russia Research Institute of Agricultural Biotechnology Moscow Russia.
AD  - Center of Molecular Biotechnology, Russian State Agrarian University-Moscow 
      Timiryazev Agricultural Academy, Timiryazevskaya str. 49, Moscow 127550, Russia 
      Russian State Agrarian University-Moscow Timiryazev Agricultural Academ Moscow 
      Russia.
FAU - Kuznetsova, Victoria M
AU  - Kuznetsova VM
AD  - Laboratory of Applied Genomics and Crop Breeding, All-Russia Research Institute 
      of Agricultural Biotechnology, Timiryazevskaya str. 42, Moscow 127550, Russia 
      All-Russia Research Institute of Agricultural Biotechnology Moscow Russia.
AD  - Center of Molecular Biotechnology, Russian State Agrarian University-Moscow 
      Timiryazev Agricultural Academy, Timiryazevskaya str. 49, Moscow 127550, Russia 
      Russian State Agrarian University-Moscow Timiryazev Agricultural Academ Moscow 
      Russia.
FAU - Nikitina, Ekaterina A
AU  - Nikitina EA
AD  - Laboratory of Applied Genomics and Crop Breeding, All-Russia Research Institute 
      of Agricultural Biotechnology, Timiryazevskaya str. 42, Moscow 127550, Russia 
      All-Russia Research Institute of Agricultural Biotechnology Moscow Russia.
FAU - Martirosyan, Yury Ts
AU  - Martirosyan YT
AD  - Group of Aeroponic Plant Growing Technologies, All-Russia Research Institute of 
      Agricultural Biotechnology, Timiryazevskaya str. 42, Moscow 127550, Russia 
      Russian State Agrarian University-Moscow Timiryazev Agricultural Acade Moscow 
      Russia.
FAU - Karlov, Gennady I
AU  - Karlov GI
AUID- ORCID: 0000-0002-9016-103X
AD  - Laboratory of Applied Genomics and Crop Breeding, All-Russia Research Institute 
      of Agricultural Biotechnology, Timiryazevskaya str. 42, Moscow 127550, Russia 
      All-Russia Research Institute of Agricultural Biotechnology Moscow Russia.
AD  - Center of Molecular Biotechnology, Russian State Agrarian University-Moscow 
      Timiryazev Agricultural Academy, Timiryazevskaya str. 49, Moscow 127550, Russia 
      Russian State Agrarian University-Moscow Timiryazev Agricultural Academ Moscow 
      Russia.
FAU - Divashuk, Mikhail G
AU  - Divashuk MG
AUID- ORCID: 0000-0001-6221-3659
AD  - Laboratory of Applied Genomics and Crop Breeding, All-Russia Research Institute 
      of Agricultural Biotechnology, Timiryazevskaya str. 42, Moscow 127550, Russia 
      All-Russia Research Institute of Agricultural Biotechnology Moscow Russia.
AD  - Center of Molecular Biotechnology, Russian State Agrarian University-Moscow 
      Timiryazev Agricultural Academy, Timiryazevskaya str. 49, Moscow 127550, Russia 
      Russian State Agrarian University-Moscow Timiryazev Agricultural Academ Moscow 
      Russia.
LA  - eng
PT  - Journal Article
DEP - 20190813
PL  - Bulgaria
TA  - Comp Cytogenet
JT  - Comparative cytogenetics
JID - 101503807
PMC - PMC6702164
OTO - NOTNLM
OT  - Thinopyrum ponticum
OT  - copy number
OT  - cytogenetic markers
OT  - fluorescence in situ hybridization
OT  - quantitative PCR
OT  - repetitive DNA
EDAT- 2019/08/24 06:00
MHDA- 2019/08/24 06:01
PMCR- 2019/08/13
CRDT- 2019/08/24 06:00
PHST- 2019/05/12 00:00 [received]
PHST- 2019/07/22 00:00 [accepted]
PHST- 2019/08/24 06:00 [entrez]
PHST- 2019/08/24 06:00 [pubmed]
PHST- 2019/08/24 06:01 [medline]
PHST- 2019/08/13 00:00 [pmc-release]
AID - 36112 [pii]
AID - 10.3897/CompCytogen.v13i3.36112 [doi]
PST - epublish
SO  - Comp Cytogenet. 2019 Aug 13;13(3):231-243. doi: 10.3897/CompCytogen.v13i3.36112. 
      eCollection 2019.